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Article

Functional Activity of Recombinant Forms of Amh and Synergistic Action with Fsh in European Sea Bass Ovary

1
Department of Fish Physiology and Biotechnology, Instituto de Acuicultura Torre de la Sal, Consejo Superior de Investigaciones Científicas (CSIC), Torre la Sal, 12595 Castellón, Spain
2
Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, Portugal
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Current address: Departamento de Medio Ambiente y Agronomía, INIA, Carretera de la Coruña Km 7.5, 28040 Madrid, Spain.
Academic Editor: Joëlle Dupont
Int. J. Mol. Sci. 2021, 22(18), 10092; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms221810092
Received: 3 August 2021 / Revised: 5 September 2021 / Accepted: 9 September 2021 / Published: 18 September 2021
(This article belongs to the Special Issue Hormones and Receptors in Sexual Reproduction)
Although anti-Müllerian hormone (AMH) has classically been correlated with the regression of Müllerian ducts in male mammals, involvement of this growth factor in other reproductive processes only recently come to light. Teleost is the only gnathostomes that lack Müllerian ducts despite having amh orthologous genes. In adult teleost gonads, Amh exerts a role in the early stages of germ cell development in both males and females. Mechanisms involving the interaction of Amh with gonadotropin- and growth factor-induced functions have been proposed, but our overall knowledge regarding Amh function in fish gonads remains modest. In this study, we report on Amh actions in the European sea bass ovary. Amh and type 2 Amh receptor (Amhr2) are present in granulosa and theca cells of both early and late-vitellogenic follicles and cannot be detected in previtellogenic ovaries. Using the Pichia pastoris system a recombinant sea bass Amh has been produced that is endogenously processed to generate a 12–15 kDa bioactive mature protein. Contrary to previous evidence in lower vertebrates, in explants of previtellogenic sea bass ovaries, mature Amh has a synergistic effect on steroidogenesis induced by the follicle-stimulating hormone (Fsh), increasing E2 and cyp19a1a levels. View Full-Text
Keywords: anti-Müllerian hormone; fish reproduction; ovary; granulosa cells; theca cells; gonadotropins; steroid hormones anti-Müllerian hormone; fish reproduction; ovary; granulosa cells; theca cells; gonadotropins; steroid hormones
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MDPI and ACS Style

Zapater, C.; Rocha, A.; Molés, G.; Mascoli, A.; Ibañez, S.; Zanuy, S.; Gómez, A. Functional Activity of Recombinant Forms of Amh and Synergistic Action with Fsh in European Sea Bass Ovary. Int. J. Mol. Sci. 2021, 22, 10092. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms221810092

AMA Style

Zapater C, Rocha A, Molés G, Mascoli A, Ibañez S, Zanuy S, Gómez A. Functional Activity of Recombinant Forms of Amh and Synergistic Action with Fsh in European Sea Bass Ovary. International Journal of Molecular Sciences. 2021; 22(18):10092. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms221810092

Chicago/Turabian Style

Zapater, Cinta, Ana Rocha, Gregorio Molés, Alessia Mascoli, Soledad Ibañez, Silvia Zanuy, and Ana Gómez. 2021. "Functional Activity of Recombinant Forms of Amh and Synergistic Action with Fsh in European Sea Bass Ovary" International Journal of Molecular Sciences 22, no. 18: 10092. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms221810092

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