Next Article in Journal
The Regulatory Roles of PPARs in Skeletal Muscle Fuel Metabolism and Inflammation: Impact of PPAR Agonism on Muscle in Chronic Disease, Contraction and Sepsis
Next Article in Special Issue
A Decellularized Human Limbal Scaffold for Limbal Stem Cell Niche Reconstruction
Previous Article in Journal
Interactions of Truncated Menaquinones in Lipid Monolayers and Bilayers
Article

FAK Shutdown: Consequences on Epithelial Morphogenesis and Biomarker Expression Involving an Innovative Biomaterial for Tissue Regeneration

1
Center for Dental Medicine, Division of Oral Biotechnology, Medical Center—University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetterstr. 55, 79106 Freiburg, Germany
2
Faculty of Biology, University of Freiburg, Schaenzlestr. 1, 79104 Freiburg, Germany
3
Center for Dental Medicine, Department of Orthodontics, Medical Center—University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetterstr. 55, 79106 Freiburg, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Emmanuel Stratakis
Int. J. Mol. Sci. 2021, 22(18), 9774; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22189774
Received: 3 August 2021 / Revised: 31 August 2021 / Accepted: 6 September 2021 / Published: 10 September 2021
(This article belongs to the Special Issue Biofabrication for Tissue Engineering Applications)
By employing an innovative biohybrid membrane, the present study aimed at elucidating the mechanistic role of the focal adhesion kinase (FAK) in epithelial morphogenesis in vitro over 4, 7, and 10 days. The consequences of siRNA-mediated FAK knockdown on epithelial morphogenesis were monitored by quantifying cell layers and detecting the expression of biomarkers of epithelial differentiation and homeostasis. Histologic examination of FAK-depleted samples showed a significant increase in cell layers resembling epithelial hyperplasia. Semiquantitative fluorescence imaging (SQFI) revealed tissue homeostatic disturbances by significantly increased involucrin expression over time, persistence of yes-associated protein (YAP) and an increase of keratin (K) 1 at day 4. The dysbalanced involucrin pattern was underscored by ROCK-IISer1366 activity at day 7 and 10. SQFI data were confirmed by quantitative PCR and Western blot analysis, thereby corroborating the FAK shutdown-related expression changes. The artificial FAK shutdown was also associated with a significantly higher expression of filaggrin at day 10, sustained keratinocyte proliferation, and the dysregulated expression of K19 and vimentin. These siRNA-induced consequences indicate the mechanistic role of FAK in epithelial morphogenesis by simultaneously considering prospective biomaterial-based epithelial regenerative approaches. View Full-Text
Keywords: epithelial morphogenesis; epithelial hyperplasia; proliferation; differentiation; focal adhesion kinase (FAK); siRNA; keratins; involucrin; filaggrin; biomaterial epithelial morphogenesis; epithelial hyperplasia; proliferation; differentiation; focal adhesion kinase (FAK); siRNA; keratins; involucrin; filaggrin; biomaterial
Show Figures

Figure 1

MDPI and ACS Style

Wang, X.; Steinberg, T.; Dieterle, M.P.; Ramminger, I.; Husari, A.; Tomakidi, P. FAK Shutdown: Consequences on Epithelial Morphogenesis and Biomarker Expression Involving an Innovative Biomaterial for Tissue Regeneration. Int. J. Mol. Sci. 2021, 22, 9774. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22189774

AMA Style

Wang X, Steinberg T, Dieterle MP, Ramminger I, Husari A, Tomakidi P. FAK Shutdown: Consequences on Epithelial Morphogenesis and Biomarker Expression Involving an Innovative Biomaterial for Tissue Regeneration. International Journal of Molecular Sciences. 2021; 22(18):9774. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22189774

Chicago/Turabian Style

Wang, Xiaoling, Thorsten Steinberg, Martin P. Dieterle, Imke Ramminger, Ayman Husari, and Pascal Tomakidi. 2021. "FAK Shutdown: Consequences on Epithelial Morphogenesis and Biomarker Expression Involving an Innovative Biomaterial for Tissue Regeneration" International Journal of Molecular Sciences 22, no. 18: 9774. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22189774

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop