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Article

The Cdc14 Phosphatase Controls Resolution of Recombination Intermediates and Crossover Formation during Meiosis

1
Center for Biological Research Margarita Salas, Department of Cellular and Molecular Biology, Spanish National Research Council (CSIC), Ramiro de Maeztu 9, 28040 Madrid, Spain
2
Department of Cell Biology, Charles University, Viničná 7, 12843 Prague, Czech Republic
3
Genome Damage and Stability Centre, University of Sussex, Brighton BN1 4DY, UK
4
Leibniz Institute for Age Research/Fritz Lipmann Institute (FLI), Beutenbergstr. 11, D-07745 Jena, Germany
5
Institute of Biochemistry, HPM D6.5-ETH Zürich, Otto-Stern-Weg 3, 8093 Zürich, Switzerland
6
Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA
7
Max Perutz Labs, University of Vienna, Dr. Bohr-Gasse 9, 1030 Vienna, Austria
8
Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK
9
Institute of Functional Biology and Genomics (IBFG), Spanish National Research Council (CSIC) and University of Salamanca, 37007 Salamanca, Spain
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Fiammetta Verni
Int. J. Mol. Sci. 2021, 22(18), 9811; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22189811
Received: 20 August 2021 / Revised: 6 September 2021 / Accepted: 8 September 2021 / Published: 10 September 2021
(This article belongs to the Special Issue DNA Damage Response (DDR) and DNA Repair)
Meiotic defects derived from incorrect DNA repair during gametogenesis can lead to mutations, aneuploidies and infertility. The coordinated resolution of meiotic recombination intermediates is required for crossover formation, ultimately necessary for the accurate completion of both rounds of chromosome segregation. Numerous master kinases orchestrate the correct assembly and activity of the repair machinery. Although much less is known, the reversal of phosphorylation events in meiosis must also be key to coordinate the timing and functionality of repair enzymes. Cdc14 is a crucial phosphatase required for the dephosphorylation of multiple CDK1 targets in many eukaryotes. Mutations that inactivate this phosphatase lead to meiotic failure, but until now it was unknown if Cdc14 plays a direct role in meiotic recombination. Here, we show that the elimination of Cdc14 leads to severe defects in the processing and resolution of recombination intermediates, causing a drastic depletion in crossovers when other repair pathways are compromised. We also show that Cdc14 is required for the correct activity and localization of the Holliday Junction resolvase Yen1/GEN1. We reveal that Cdc14 regulates Yen1 activity from meiosis I onwards, and this function is essential for crossover resolution in the absence of other repair pathways. We also demonstrate that Cdc14 and Yen1 are required to safeguard sister chromatid segregation during the second meiotic division, a late action that is independent of the earlier role in crossover formation. Thus, this work uncovers previously undescribed functions of the evolutionary conserved Cdc14 phosphatase in the regulation of meiotic recombination. View Full-Text
Keywords: Cdc14; Yen1; Sgs1; Mus81; CDK1; Ndt80; Cdc5; Cdc20; meiotic recombination; aneuploidy; Holliday junction Cdc14; Yen1; Sgs1; Mus81; CDK1; Ndt80; Cdc5; Cdc20; meiotic recombination; aneuploidy; Holliday junction
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MDPI and ACS Style

Alonso-Ramos, P.; Álvarez-Melo, D.; Strouhalova, K.; Pascual-Silva, C.; Garside, G.B.; Arter, M.; Bermejo, T.; Grigaitis, R.; Wettstein, R.; Fernández-Díaz, M.; Matos, J.; Geymonat, M.; San-Segundo, P.A.; Carballo, J.A. The Cdc14 Phosphatase Controls Resolution of Recombination Intermediates and Crossover Formation during Meiosis. Int. J. Mol. Sci. 2021, 22, 9811. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22189811

AMA Style

Alonso-Ramos P, Álvarez-Melo D, Strouhalova K, Pascual-Silva C, Garside GB, Arter M, Bermejo T, Grigaitis R, Wettstein R, Fernández-Díaz M, Matos J, Geymonat M, San-Segundo PA, Carballo JA. The Cdc14 Phosphatase Controls Resolution of Recombination Intermediates and Crossover Formation during Meiosis. International Journal of Molecular Sciences. 2021; 22(18):9811. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22189811

Chicago/Turabian Style

Alonso-Ramos, Paula, David Álvarez-Melo, Katerina Strouhalova, Carolina Pascual-Silva, George B. Garside, Meret Arter, Teresa Bermejo, Rokas Grigaitis, Rahel Wettstein, Marta Fernández-Díaz, Joao Matos, Marco Geymonat, Pedro A. San-Segundo, and Jesús A. Carballo 2021. "The Cdc14 Phosphatase Controls Resolution of Recombination Intermediates and Crossover Formation during Meiosis" International Journal of Molecular Sciences 22, no. 18: 9811. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22189811

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