Next Article in Journal
Multiple Roles for Cholinergic Signaling from the Perspective of Stem Cell Function
Next Article in Special Issue
Evaluation of Nanotargeted 111In-Cyclic RGDfK-Liposome in a Human Melanoma Xenotransplantation Model
Previous Article in Journal
TGF-β Activity of a Demineralized Bone Matrix
Previous Article in Special Issue
Delivery of the Radionuclide 131I Using Cationic Fusogenic Liposomes as Nanocarriers
Article

Hyaluronan-Loaded Liposomal Dexamethasone–Diclofenac Nanoparticles for Local Osteoarthritis Treatment

1
Isotope Application Division, Institute of Nuclear Energy Research, P.O. Box 3-27, Longtan, Taoyuan 325, Taiwan
2
Department of Obstetrics and Gynecology, Medicine College of Medicine, National Taiwan University, Taipei 100, Taiwan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(2), 665; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22020665
Received: 17 December 2020 / Revised: 6 January 2021 / Accepted: 8 January 2021 / Published: 11 January 2021
(This article belongs to the Special Issue Functionalized Liposomes)
Osteoarthritis (OA) remains one of the common degenerative joint diseases and a major cause of pain and disability in older adult individuals. Oral administration of non-steroidal anti-inflammatory drugs (NSAIDs) (such as diclofenac, DIC) or intra-articular injected gluco-corticosteroids (such as dexamethasone, DEX) were the conventional treatment strategies for OA to reduce joint pain. Current limitations for both drugs including severe adverse effects with risks of toxicity were noted. The aim of the present study was to generate a novel OA treatment formulation hyaluronic acid (HA)-Liposomal (Lipo)-DIC/DEX to combat joint pain. The formulation was prepared by constructing DIC with DEX-loaded nanostructured lipid carriers Lipo-DIC/DEX mixed with hyaluronic acid (HA) for prolonged OA application. The prepared Lipo-DIC/DEX nanoparticles revealed the size as 103.6 ± 0.3 nm on average, zeta potential as −22.3 ± 4.6 mV, the entrapment efficiency of 90.5 ± 5.6%, and the DIC and DEX content was 22.5 ± 4.1 and 2.5 ± 0.6%, respectively. Evidence indicated that HA-Lipo-DIC/DEX could reach the effective working concentration in 4 h and sustained the drug-releasing time for at least 168 h. No significant toxicities but increased cell numbers were observed when HA-Lipo-DIC/DEX co-cultured with articular chondrocytes cells. Using live-animal In vivo imaging system (IVIS), intra-articular injection of each HA-Lipo-DIC/DEX sufficed to reduce knee joint inflammation in OA mice over a time span of four weeks. Single-dose injection could reduce the inflammation volume down to 77.5 ± 5.1% from initial over that time span. Our results provided the novel drug-releasing formulation with safety and efficiency which could be a promising system for osteoarthritis pain control. View Full-Text
Keywords: liposomal nanoparticle; dexamethasone; diclofenac; osteoarthritis liposomal nanoparticle; dexamethasone; diclofenac; osteoarthritis
Show Figures

Figure 1

MDPI and ACS Style

Chang, M.-C.; Chiang, P.-F.; Kuo, Y.-J.; Peng, C.-L.; Chen, K.-Y.; Chiang, Y.-C. Hyaluronan-Loaded Liposomal Dexamethasone–Diclofenac Nanoparticles for Local Osteoarthritis Treatment. Int. J. Mol. Sci. 2021, 22, 665. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22020665

AMA Style

Chang M-C, Chiang P-F, Kuo Y-J, Peng C-L, Chen K-Y, Chiang Y-C. Hyaluronan-Loaded Liposomal Dexamethasone–Diclofenac Nanoparticles for Local Osteoarthritis Treatment. International Journal of Molecular Sciences. 2021; 22(2):665. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22020665

Chicago/Turabian Style

Chang, Ming-Cheng, Ping-Fang Chiang, Yu-Jen Kuo, Cheng-Liang Peng, Kuan-Yin Chen, and Ying-Cheng Chiang. 2021. "Hyaluronan-Loaded Liposomal Dexamethasone–Diclofenac Nanoparticles for Local Osteoarthritis Treatment" International Journal of Molecular Sciences 22, no. 2: 665. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22020665

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop