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Article

Venous and Arterial Endothelial Cells from Human Umbilical Cords: Potential Cell Sources for Cardiovascular Research

1
Institute of Active Polymers and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, 14513 Teltow, Germany
2
Institute of Chemistry, University of Potsdam, 14476 Potsdam, Germany
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(2), 978; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22020978
Received: 17 December 2020 / Revised: 9 January 2021 / Accepted: 13 January 2021 / Published: 19 January 2021
(This article belongs to the Special Issue Future Trends in Biomaterials and Devices for Cells and Tissues)
Although cardiovascular devices are mostly implanted in arteries or to replace arteries, in vitro studies on implant endothelialization are commonly performed with human umbilical cord-derived venous endothelial cells (HUVEC). In light of considerable differences, both morphologically and functionally, between arterial and venous endothelial cells, we here compare HUVEC and human umbilical cord-derived arterial endothelial cells (HUAEC) regarding their equivalence as an endothelial cell in vitro model for cardiovascular research. No differences were found in either for the tested parameters. The metabolic activity and lactate dehydrogenase, an indicator for the membrane integrity, slightly decreased over seven days of cultivation upon normalization to the cell number. The amount of secreted nitrite and nitrate, as well as prostacyclin per cell, also decreased slightly over time. Thromboxane B2 was secreted in constant amounts per cell at all time points. The Von Willebrand factor remained mainly intracellularly up to seven days of cultivation. In contrast, collagen and laminin were secreted into the extracellular space with increasing cell density. Based on these results one might argue that both cell types are equally suited for cardiovascular research. However, future studies should investigate further cell functionalities, and whether arterial endothelial cells from implantation-relevant areas, such as coronary arteries in the heart, are superior to umbilical cord-derived endothelial cells. View Full-Text
Keywords: human umbilical cord-derived endothelial cells; cardiovascular research; cardiovascular implants; artery endothelial cell compatibility testing human umbilical cord-derived endothelial cells; cardiovascular research; cardiovascular implants; artery endothelial cell compatibility testing
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MDPI and ACS Style

Lau, S.; Gossen, M.; Lendlein, A.; Jung, F. Venous and Arterial Endothelial Cells from Human Umbilical Cords: Potential Cell Sources for Cardiovascular Research. Int. J. Mol. Sci. 2021, 22, 978. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22020978

AMA Style

Lau S, Gossen M, Lendlein A, Jung F. Venous and Arterial Endothelial Cells from Human Umbilical Cords: Potential Cell Sources for Cardiovascular Research. International Journal of Molecular Sciences. 2021; 22(2):978. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22020978

Chicago/Turabian Style

Lau, Skadi, Manfred Gossen, Andreas Lendlein, and Friedrich Jung. 2021. "Venous and Arterial Endothelial Cells from Human Umbilical Cords: Potential Cell Sources for Cardiovascular Research" International Journal of Molecular Sciences 22, no. 2: 978. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22020978

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