Irradiation Activates MZF1 to Inhibit miR-541-5p Expression and Promote Epithelial-Mesenchymal Transition (EMT) in Radiation-Induced Pulmonary Fibrosis (RIPF) by Upregulating Slug
Abstract
:1. Introduction
2. Results
2.1. Ionizing Radiation Can Reduce the Expression of miR-541-5p
2.2. Knockdown of miR-541-5p Could Promote the Occurrence of EMT
2.3. miR-541-5p Directly Suppressed Slug via Binding to the 3′-UTR Region
2.4. miR-541-5p Negatively Regulated EMT by Inhibiting Slug
2.5. IR Downgrades miR-541-5p via MZF1
2.6. MZF1 Promotes EMT via Repression of miR-541-5p Following IR
2.7. MZF1 Mediates RIPF via miR-541-5p/Slug Axis
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. RNA Isolation, Reverse Transcription, and qRT-PCR
4.3. Irradiation and Transfection
4.4. Mice and Mice Treatment
4.5. Western Blot Analysis and Antibodies
4.6. Immunofluorescence Analysis
4.7. Dual-Luciferase Reporter Gene Assay
4.8. Hematoxylin and Eosin (H&E) and Masson’s Triple Stain
4.9. Immunohistochemistry (IHC) Assay
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primer ID | Sequence (5′-3′) |
---|---|
Has-miR-541-5p-F | AGGATTCTGCTGTCGGT |
Has-miR-541-5p-R | GGTCCAGTTTTTTTTTTTTTTTAGTG |
Slug-F | GACTGACCCGTCGTGACG |
Slug-R | GCAGACGACGGGTCAGAT |
Pri-miR-541-5p-F | ACGGTGCATGTCATCTGTTC |
Pri-miR-541-5p-R | AAGATGTCACAGACGACTTC |
MZF1-F | GGGCCTGCAGGTGAAAGAG |
MZF1-R | GGCAGCTAGAGGCCCAGACT |
Has-U6-F | ATTGGAACGATACAGAGAAGAAT |
Has-U6-R | GGAACGCTTCACGAATTTG |
β-actin-F | GAATCAATGCAAGTTCGGTTCC |
β-actin-R | TCATCTCCGCTATTAGCTCCG |
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Liang, X.; Yan, Z.; Wang, P.; Liu, Y.; Ao, X.; Liu, Z.; Wang, D.; Liu, X.; Zhu, M.; Gao, S.; et al. Irradiation Activates MZF1 to Inhibit miR-541-5p Expression and Promote Epithelial-Mesenchymal Transition (EMT) in Radiation-Induced Pulmonary Fibrosis (RIPF) by Upregulating Slug. Int. J. Mol. Sci. 2021, 22, 11309. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222111309
Liang X, Yan Z, Wang P, Liu Y, Ao X, Liu Z, Wang D, Liu X, Zhu M, Gao S, et al. Irradiation Activates MZF1 to Inhibit miR-541-5p Expression and Promote Epithelial-Mesenchymal Transition (EMT) in Radiation-Induced Pulmonary Fibrosis (RIPF) by Upregulating Slug. International Journal of Molecular Sciences. 2021; 22(21):11309. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222111309
Chicago/Turabian StyleLiang, Xinxin, Ziyan Yan, Ping Wang, Yuhao Liu, Xingkun Ao, Zheng Liu, Duo Wang, Xiaochang Liu, Maoxiang Zhu, Shanshan Gao, and et al. 2021. "Irradiation Activates MZF1 to Inhibit miR-541-5p Expression and Promote Epithelial-Mesenchymal Transition (EMT) in Radiation-Induced Pulmonary Fibrosis (RIPF) by Upregulating Slug" International Journal of Molecular Sciences 22, no. 21: 11309. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222111309