Connexin46 Expression Enhances Cancer Stem Cell and Epithelial-to-Mesenchymal Transition Characteristics of Human Breast Cancer MCF-7 Cells
Abstract
:1. Introduction
2. Results
2.1. Over-Expression of Cx46 Does Not Modify the Rate of MCF-7 Cell Division
2.2. Cx46 Increases the Expression of CSC Related Genes and Funtional Characteristics
2.3. Cx46 Increases the Expression of EMT Related Genes and Functional Characteristics
2.4. Cx46 Increases the Release of VEGF and Promote Tube Formation in Endothelial Cells
3. Discussion
4. Materials and Methods
4.1. Plasmid and Cells
4.2. Western Blot
4.3. Clonogenic Assay
4.4. Sphere Assay
4.5. Migration Assay
4.6. Transwell Invasion Assay
4.7. Angiogenesis Assay
4.8. Luminex Assay
4.9. Reverse Transcription-Quantitative Polymerase Chain Reaction
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Acuña, R.A.; Varas-Godoy, M.; Herrera-Sepulveda, D.; Retamal, M.A. Connexin46 Expression Enhances Cancer Stem Cell and Epithelial-to-Mesenchymal Transition Characteristics of Human Breast Cancer MCF-7 Cells. Int. J. Mol. Sci. 2021, 22, 12604. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212604
Acuña RA, Varas-Godoy M, Herrera-Sepulveda D, Retamal MA. Connexin46 Expression Enhances Cancer Stem Cell and Epithelial-to-Mesenchymal Transition Characteristics of Human Breast Cancer MCF-7 Cells. International Journal of Molecular Sciences. 2021; 22(22):12604. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212604
Chicago/Turabian StyleAcuña, Rodrigo A., Manuel Varas-Godoy, Diego Herrera-Sepulveda, and Mauricio A. Retamal. 2021. "Connexin46 Expression Enhances Cancer Stem Cell and Epithelial-to-Mesenchymal Transition Characteristics of Human Breast Cancer MCF-7 Cells" International Journal of Molecular Sciences 22, no. 22: 12604. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms222212604