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Open AccessArticle

The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells

1
The Shraga Segal Department of Microbiology, Immunology, and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel
2
The Center of Advanced Research and Education in Reproduction (CARER), Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel
3
Department of OB/GYN, Soroka Medical Center, Beer Sheva 8410501, Israel
*
Author to whom correspondence should be addressed.
Academic Editor: Youngsok Choi
Int. J. Mol. Sci. 2021, 22(3), 1147; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22031147
Received: 22 December 2020 / Revised: 12 January 2021 / Accepted: 21 January 2021 / Published: 24 January 2021
(This article belongs to the Special Issue The Development, Differentiation, and Toxicity of Gametes)
Pigment epithelium derived factor (PEDF) is a multifunctional secretory soluble glycoprotein that belongs to the serine protease inhibitor (serpin) family. It was reported to have neurotrophic, anti-angiogenic and anti-tumorigenic activity. Recently, PEDF was found in testicular peritubular cells and it was assumed to be involved in the avascular nature of seminiferous tubules. The aim of this study was to determine the cellular origin, expression levels and target cells of PEDF in testicular tissue of immature and adult mice under physiological conditions, and to explore its possible role in the process of spermatogenesis in vitro. Using immunofluorescence staining, we showed that PEDF was localized in spermatogenic cells at different stages of development as well as in the somatic cells of the testis. Its protein levels in testicular homogenates and Sertoli cells supernatant showed a significant decrease with age. PEDF receptor (PEDF-R) was localized within the seminiferous tubule cells and in the interstitial cells compartment. Its RNA expression levels showed an increase with age until 8 weeks followed by a decrease. RNA levels of PEDF-R showed the opposite trend of the protein. Addition of PEDF to cultures of isolated cells from the seminiferous tubules did not changed their proliferation rate, however, a significant increase was observed in number of meiotic/post meiotic cells at 1000 ng/mL of PEDF; indicating an in vitro differentiation effect. This study may suggest a role for PEDF in the process of spermatogenesis. View Full-Text
Keywords: testis; spermatogenesis; in vitro differentiation of spermatogonial cells; pigment epithelium derived factor (PEDF); somatic cells; male fertility; 3D in vitro culture system testis; spermatogenesis; in vitro differentiation of spermatogonial cells; pigment epithelium derived factor (PEDF); somatic cells; male fertility; 3D in vitro culture system
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MDPI and ACS Style

Bagdadi, N.; Sawaied, A.; AbuMadighem, A.; Lunenfeld, E.; Huleihel, M. The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells. Int. J. Mol. Sci. 2021, 22, 1147. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22031147

AMA Style

Bagdadi N, Sawaied A, AbuMadighem A, Lunenfeld E, Huleihel M. The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells. International Journal of Molecular Sciences. 2021; 22(3):1147. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22031147

Chicago/Turabian Style

Bagdadi, Noy; Sawaied, Alaa; AbuMadighem, Ali; Lunenfeld, Eitan; Huleihel, Mahmoud. 2021. "The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells" Int. J. Mol. Sci. 22, no. 3: 1147. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22031147

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