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Effects of Physiological Doses of Resveratrol and Quercetin on Glucose Metabolism in Primary Myotubes

Nutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy, University of Basque Country (UPV/EHU) and Lucio Lascaray Research Centre, 01006 Vitoria, Spain
Bioaraba Health Research Institute, 01009 Vitoria, Spain
CIBEROBN Physiopathology of Obesity and Nutrition, Institute of Health Carlos III (ISCIII), 28029 Madrid, Spain
INSERM, UMR1048, Obesity Research Laboratory, Institute of Metabolic and Cardiovascular Diseases, 31432 Toulouse, France
Authors to whom correspondence should be addressed.
Academic Editor: Marie-Laure Fauconnier
Int. J. Mol. Sci. 2021, 22(3), 1384;
Received: 12 January 2021 / Revised: 28 January 2021 / Accepted: 29 January 2021 / Published: 30 January 2021
Phenolic compounds have emerged in recent years as an option to face insulin resistance and diabetes. The central aim of this study was: (1) to demonstrate that physiological doses of resveratrol (RSV) or quercetin (Q) can influence glucose metabolism in human myotubes, (2) to establish whether AMP-activated protein kinase (AMPK) and protein kinase B –PKB- (Akt) pathways are involved in this effect. In addition, the effects of these polyphenols on mitochondrial biogenesis and fatty acid oxidation were analysed. Myotubes from healthy donors were cultured for 24 h with either 0.1 μM of RSV or with 10 μM of Q. Glucose metabolism, such as glycogen synthesis, glucose oxidation, and lactate production, were measured with D[U-14C]glucose. β-oxidation using [1–14C]palmitate as well as the expression of key metabolic genes and proteins by Real Time PCR and Western blot were also assessed. Although RSV and Q increased pgc1α expression, they did not significantly change either glucose oxidation or β-oxidation. Q increased AMPK, insulin receptor substrate 1 (IRS-1), and AS160 phosphorylation in basal conditions and glycogen synthase kinase 3 (GSK3β) in insulin-stimulated conditions. RSV tended to increase the phosphorylation rates of AMPK and GSK3β. Both of the polyphenols increased insulin-stimulated glycogen synthesis and reduced lactate production in human myotubes. Thus, physiological doses of RSV or Q may exhibit anti-diabetic actions in human myotubes. View Full-Text
Keywords: resveratrol; quercetin; glucose; human primary myotubes resveratrol; quercetin; glucose; human primary myotubes
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MDPI and ACS Style

Eseberri, I.; Laurens, C.; Miranda, J.; Louche, K.; Lasa, A.; Moro, C.; Portillo, M.P. Effects of Physiological Doses of Resveratrol and Quercetin on Glucose Metabolism in Primary Myotubes. Int. J. Mol. Sci. 2021, 22, 1384.

AMA Style

Eseberri I, Laurens C, Miranda J, Louche K, Lasa A, Moro C, Portillo MP. Effects of Physiological Doses of Resveratrol and Quercetin on Glucose Metabolism in Primary Myotubes. International Journal of Molecular Sciences. 2021; 22(3):1384.

Chicago/Turabian Style

Eseberri, Itziar, Claire Laurens, Jonatan Miranda, Katie Louche, Arrate Lasa, Cedric Moro, and Maria P. Portillo 2021. "Effects of Physiological Doses of Resveratrol and Quercetin on Glucose Metabolism in Primary Myotubes" International Journal of Molecular Sciences 22, no. 3: 1384.

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