Next Article in Journal
Hyperglycemic Condition Causes Pro-Inflammatory and Permeability Alterations Associated with Monocyte Recruitment and Deregulated NFκB/PPARγ Pathways on Cerebral Endothelial Cells: Evidence for Polyphenols Uptake and Protective Effect
Next Article in Special Issue
Bioactive Alkaloids from Genus Aspergillus: Mechanistic Interpretation of Their Antimicrobial and Potential SARS-CoV-2 Inhibitory Activity Using Molecular Modelling
Previous Article in Journal
Urinary Levels of the Acrolein Conjugates of Carnosine Are Associated with Cardiovascular Disease Risk
Previous Article in Special Issue
A Recent Insight Regarding the Phytochemistry and Bioactivity of Origanum vulgare L. Essential Oil
Article

Effects of Physiological Doses of Resveratrol and Quercetin on Glucose Metabolism in Primary Myotubes

1
Nutrition and Obesity Group, Department of Nutrition and Food Science, Faculty of Pharmacy, University of Basque Country (UPV/EHU) and Lucio Lascaray Research Centre, 01006 Vitoria, Spain
2
Bioaraba Health Research Institute, 01009 Vitoria, Spain
3
CIBEROBN Physiopathology of Obesity and Nutrition, Institute of Health Carlos III (ISCIII), 28029 Madrid, Spain
4
INSERM, UMR1048, Obesity Research Laboratory, Institute of Metabolic and Cardiovascular Diseases, 31432 Toulouse, France
*
Authors to whom correspondence should be addressed.
Academic Editor: Marie-Laure Fauconnier
Int. J. Mol. Sci. 2021, 22(3), 1384; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22031384
Received: 12 January 2021 / Revised: 28 January 2021 / Accepted: 29 January 2021 / Published: 30 January 2021
Phenolic compounds have emerged in recent years as an option to face insulin resistance and diabetes. The central aim of this study was: (1) to demonstrate that physiological doses of resveratrol (RSV) or quercetin (Q) can influence glucose metabolism in human myotubes, (2) to establish whether AMP-activated protein kinase (AMPK) and protein kinase B –PKB- (Akt) pathways are involved in this effect. In addition, the effects of these polyphenols on mitochondrial biogenesis and fatty acid oxidation were analysed. Myotubes from healthy donors were cultured for 24 h with either 0.1 μM of RSV or with 10 μM of Q. Glucose metabolism, such as glycogen synthesis, glucose oxidation, and lactate production, were measured with D[U-14C]glucose. β-oxidation using [1–14C]palmitate as well as the expression of key metabolic genes and proteins by Real Time PCR and Western blot were also assessed. Although RSV and Q increased pgc1α expression, they did not significantly change either glucose oxidation or β-oxidation. Q increased AMPK, insulin receptor substrate 1 (IRS-1), and AS160 phosphorylation in basal conditions and glycogen synthase kinase 3 (GSK3β) in insulin-stimulated conditions. RSV tended to increase the phosphorylation rates of AMPK and GSK3β. Both of the polyphenols increased insulin-stimulated glycogen synthesis and reduced lactate production in human myotubes. Thus, physiological doses of RSV or Q may exhibit anti-diabetic actions in human myotubes. View Full-Text
Keywords: resveratrol; quercetin; glucose; human primary myotubes resveratrol; quercetin; glucose; human primary myotubes
Show Figures

Graphical abstract

MDPI and ACS Style

Eseberri, I.; Laurens, C.; Miranda, J.; Louche, K.; Lasa, A.; Moro, C.; Portillo, M.P. Effects of Physiological Doses of Resveratrol and Quercetin on Glucose Metabolism in Primary Myotubes. Int. J. Mol. Sci. 2021, 22, 1384. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22031384

AMA Style

Eseberri I, Laurens C, Miranda J, Louche K, Lasa A, Moro C, Portillo MP. Effects of Physiological Doses of Resveratrol and Quercetin on Glucose Metabolism in Primary Myotubes. International Journal of Molecular Sciences. 2021; 22(3):1384. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22031384

Chicago/Turabian Style

Eseberri, Itziar, Claire Laurens, Jonatan Miranda, Katie Louche, Arrate Lasa, Cedric Moro, and Maria P. Portillo 2021. "Effects of Physiological Doses of Resveratrol and Quercetin on Glucose Metabolism in Primary Myotubes" International Journal of Molecular Sciences 22, no. 3: 1384. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22031384

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop