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Article

Transgenic Mice Overexpressing PG1 Display Corneal Opacity and Severe Inflammation in the Eye

1
Department of Stem Cell Biology and Regenerative Biology, Konkuk University, Hwayang-dong, Seoul 05029, Korea
2
College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Korea
3
Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Loredana Frasca
Int. J. Mol. Sci. 2021, 22(4), 1586; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22041586
Received: 5 December 2020 / Revised: 28 January 2021 / Accepted: 29 January 2021 / Published: 4 February 2021
(This article belongs to the Special Issue Antimicrobial Peptides and Antimicrobial Chemokines)
Antimicrobial peptides (AMPs) are of interest as alternatives to antibiotics or immunomodulators. We generated and characterized the phenotypes of transgenic mice overexpressing protegrin 1 (PG1), a potent porcine cathelicidin. No obvious differences were observed between PG1 transgenic and wild-type mice in terms of growth, development, general behaviour, and the major immune cell population. However, PG1 transgenic mice intranasally infected with Staphylococcus aureus resulted in a reduction in microscopic pulmonary injury, improved clearance of bacteria, and lower proinflammatory cytokine secretion, compared to those of wild-type mice. On the other hand, approximately 25% of PG1 transgenic mice (n = 54/215) showed corneal opacity and developed inflammation in the eye, resulting ultimately in phthisis bulbi. Immunohistochemical analyses revealed that PG1 and its activator, neutrophil elastase, localized to the basal cells of the cornea and glands in eyelids, respectively. In addition, apoptosis indicated by a Terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL)-positive signal was detected from flat cells of the cornea. Our study suggests that the expression regulation or localization of AMPs such as PG1 is important to prevent their adverse effects. However, our results also showed that the cytotoxic effects of PG1 on cells could be tolerated in animals, except for the eyes. View Full-Text
Keywords: protegrin; antimicrobial peptide; cathelicidin; transgenic mice; corneal opacity protegrin; antimicrobial peptide; cathelicidin; transgenic mice; corneal opacity
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MDPI and ACS Style

Choi, M.-K.; Le, M.T.; Cho, H.-S.; Lee, J.; Jeon, H.; Cha, S.-Y.; Na, M.; Chun, T.; Kim, J.-H.; Song, H.; Park, C. Transgenic Mice Overexpressing PG1 Display Corneal Opacity and Severe Inflammation in the Eye. Int. J. Mol. Sci. 2021, 22, 1586. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22041586

AMA Style

Choi M-K, Le MT, Cho H-S, Lee J, Jeon H, Cha S-Y, Na M, Chun T, Kim J-H, Song H, Park C. Transgenic Mice Overexpressing PG1 Display Corneal Opacity and Severe Inflammation in the Eye. International Journal of Molecular Sciences. 2021; 22(4):1586. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22041586

Chicago/Turabian Style

Choi, Min-Kyeung, Minh T. Le, Hye-Sun Cho, Juyoung Lee, Hyoim Jeon, Se-Yeoun Cha, Manheum Na, Taehoon Chun, Jin-Hoi Kim, Hyuk Song, and Chankyu Park. 2021. "Transgenic Mice Overexpressing PG1 Display Corneal Opacity and Severe Inflammation in the Eye" International Journal of Molecular Sciences 22, no. 4: 1586. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22041586

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