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Article

Heightened Crescentic Glomerulonephritis in Immune Challenged 129sv Mice Is TGF-β/Smad3 Dependent

1
The Department of Biomedical Engineering, University of Houston, Houston, TX 77204-5060, USA
2
The Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390-0000, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Gian Marco Ghiggeri
Int. J. Mol. Sci. 2021, 22(4), 2059; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22042059
Received: 14 January 2021 / Revised: 14 February 2021 / Accepted: 17 February 2021 / Published: 19 February 2021
(This article belongs to the Section Molecular Endocrinology and Metabolism)
The 129sv mouse strain is particularly sensitive to experimental immune-mediated nephritis. Previous studies have indicated that transforming growth factor-β (TGF-β) plays a critical role in both immune modulation and tissue fibrogenesis in various diseases and that its biological activities are exerted via the SMAD family. In this study, we aimed to determine whether TGF-β/SMAD signaling is essential for the development of immune-mediated nephritis in 129sv mice. Relative to C57BL/6J control mice with anti-glomeruli basement membrane (GBM) nephritis, 129sv mice with anti-GBM nephritis exhibited increased renal collagen deposition. Additionally, higher mRNA levels of pro-collagen and collagen IV, higher serum levels of active and total TGF-β1, and increased TGF-β1, TGF-βIIR, and phosphorylated SMAD expression were detected in these mice. Deletion of Smad3 in 129sv mice ameliorated anti-GBM induced nephritis, including crescentic glomerulonephritis. Collectively, these findings indicate that the heightened experimental nephritis and fibrotic disease in the 129sv strain of mice are regulated by SMAD3, which could be a potential therapeutic target for immune-mediated nephritis. View Full-Text
Keywords: anti-GBM nephritis; TGF-β/SMAD signaling; 129sv strain anti-GBM nephritis; TGF-β/SMAD signaling; 129sv strain
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MDPI and ACS Style

Du, Y.; Xie, C.; Ravikumar, S.; Orme, J.; Li, L.; Zhou, X.J.; Mohan, C. Heightened Crescentic Glomerulonephritis in Immune Challenged 129sv Mice Is TGF-β/Smad3 Dependent. Int. J. Mol. Sci. 2021, 22, 2059. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22042059

AMA Style

Du Y, Xie C, Ravikumar S, Orme J, Li L, Zhou XJ, Mohan C. Heightened Crescentic Glomerulonephritis in Immune Challenged 129sv Mice Is TGF-β/Smad3 Dependent. International Journal of Molecular Sciences. 2021; 22(4):2059. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22042059

Chicago/Turabian Style

Du, Yong, Chun Xie, Sneha Ravikumar, Jacob Orme, Li Li, Xin J. Zhou, and Chandra Mohan. 2021. "Heightened Crescentic Glomerulonephritis in Immune Challenged 129sv Mice Is TGF-β/Smad3 Dependent" International Journal of Molecular Sciences 22, no. 4: 2059. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22042059

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