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Article

Global Changes of 5-mC/5h-mC Ratio and Methylation of Adiponectin and Leptin Gene in Placenta Depending on Mode of Delivery

1
First Department of Obstetrics and Gynaecology, Centre of Postgraduate Medical Education, Żelazna 90, 01-004 Warsaw, Poland
2
Department of Medical Biology and Genetics, Faculty of Biology University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland
3
Department of Public Health, Medical University of Warsaw, Nielubowicza 5, 02-097 Warsaw, Poland
4
Department of Women’s Health, Institute of Rural Health in Lublin, Jaczewskiego 2, 20-950 Lublin, Poland
5
Department of Medical Statistics, School of Public Health, Center of Postgraduate Medical Education, Kleczewska 61/63, 01-826 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Daniel Vaiman
Int. J. Mol. Sci. 2021, 22(6), 3195; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22063195
Received: 18 February 2021 / Revised: 8 March 2021 / Accepted: 18 March 2021 / Published: 21 March 2021
(This article belongs to the Special Issue Reproductive Immunology and Pregnancy)
It was suggested that the epigenetic alterations of the placenta are associated with obesity, as well as the delivery mode. This study aimed to assess the effect of maternal outcome and delivery procedure on global placental DNA methylation status, as well as selected 5’-Cytosine-phosphate-Guanine-3’ (CpG) sites in ADIPOQ and LEP genes. Global DNA methylation profile in the placenta was assessed using the 5-methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-hmC) ratio evaluated with the ELISA, followed by target gene methylation patterns at selected gene regions which were determined using methylation-specific qPCR in 70 placentas from healthy, pregnant women with single pregnancy. We found no statistically significant differences in 5-mC/5-hmC ratio between intrapartum cesarean sections (CS) and vaginal deliveries (p = 0.214), as well as between elective cesarean sections and vaginal deliveries (p = 0.221). In intrapartum cesarean sections, the ADIPOQ demethylation index was significantly higher (the average: 1.75) compared to elective cesarean section (the average: 1.23, p = 0.010) and vaginal deliveries (the average: 1.23, p = 0.011). The LEP demethylation index did not significantly differ among elective CS, intrapartum CS, and vaginal delivery groups. The demethylation index of ADIPOQ correlated negatively with LEP in the placenta in the vaginal delivery group (r = −0.456, p = 0.017), but not with the global methylation. The methylation of a singular locus might be different depending on the mode of delivery and uterine contractions. Further studies should be conducted with locus-specific analysis of the whole genome to detect the methylation index of specific genes involved in metabolism. View Full-Text
Keywords: cesarean section; adipokines; DNA methylation; 5-mC/5-hmC ratio; ADIPOQ; LEP; placenta cesarean section; adipokines; DNA methylation; 5-mC/5-hmC ratio; ADIPOQ; LEP; placenta
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MDPI and ACS Style

Słabuszewska-Jóźwiak, A.; Malinowska, M.; Kloska, A.; Jakóbkiewicz-Banecka, J.; Gujski, M.; Bojar, I.; Raczkiewicz, D.; Jakiel, G. Global Changes of 5-mC/5h-mC Ratio and Methylation of Adiponectin and Leptin Gene in Placenta Depending on Mode of Delivery. Int. J. Mol. Sci. 2021, 22, 3195. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22063195

AMA Style

Słabuszewska-Jóźwiak A, Malinowska M, Kloska A, Jakóbkiewicz-Banecka J, Gujski M, Bojar I, Raczkiewicz D, Jakiel G. Global Changes of 5-mC/5h-mC Ratio and Methylation of Adiponectin and Leptin Gene in Placenta Depending on Mode of Delivery. International Journal of Molecular Sciences. 2021; 22(6):3195. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22063195

Chicago/Turabian Style

Słabuszewska-Jóźwiak, Aneta, Marcelina Malinowska, Anna Kloska, Joanna Jakóbkiewicz-Banecka, Mariusz Gujski, Iwona Bojar, Dorota Raczkiewicz, and Grzegorz Jakiel. 2021. "Global Changes of 5-mC/5h-mC Ratio and Methylation of Adiponectin and Leptin Gene in Placenta Depending on Mode of Delivery" International Journal of Molecular Sciences 22, no. 6: 3195. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22063195

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