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The Role of TRPA1 in Skin Physiology and Pathology
Article

Transient Receptor Potential Ankyrin 1 (TRPA1)—An Inflammation-Induced Factor in Human HaCaT Keratinocytes

The Immunopharmacology Research Group, Faculty of Medicine and Health Technology, Tampere University and Tampere University Hospital, 33014 Tampere, Finland
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Academic Editors: Viktorie Vlachova and Peter M. Zygmunt
Int. J. Mol. Sci. 2021, 22(7), 3322; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22073322
Received: 8 February 2021 / Revised: 10 March 2021 / Accepted: 17 March 2021 / Published: 24 March 2021
(This article belongs to the Special Issue TRPA1 Channel)
Transient receptor potential ankyrin 1 (TRPA1) is an ion channel mainly studied in sensory neurons where it mediates itch, pain and neurogenic inflammation. Recently, some nonneuronal cells have also been shown to express TRPA1 to support inflammatory responses. To address the role of TRPA1 in skin inflammation, we aimed to investigate TRPA1 expression in keratinocytes. HaCaT cells (a model of human keratinocytes) and skin biopses from wild-type and TRPA1 deficient mice were used in the studies. TRPA1 expression in nonstimulated keratinocytes was very low but significantly inducible by the proinflammatory cytokine tumor necrosis factor (TNF) in an nuclear factor kappa B (NF-κB), and mitogen-activated protein (MAP) kinase (p38 and c-Jun N-terminal kinase, JNK)-dependent manner. Interestingly, drugs widely used to treat skin inflammation, the calcineurin inhibitors tacrolimus and cyclosporine and the glucocorticoid dexamethasone, significantly decreased TRPA1 expression. Furthermore, pharmacological inhibition and genetic deletion of TRPA1 reduced the synthesis of TNF-induced monocyte chemoattractant protein 1 (MCP-1) in keratinocytes and mouse skin biopsies. In conclusion, these findings point to an inflammatory role for TRPA1 in keratinocytes and present TRPA1 as a potential drug target in inflammatory skin diseases. View Full-Text
Keywords: transient receptor potential ankyrin 1 (TRPA1) cation channel; inflammation; tumor necrosis factor (TNF); calcineurin inhibitors; glucocorticoids transient receptor potential ankyrin 1 (TRPA1) cation channel; inflammation; tumor necrosis factor (TNF); calcineurin inhibitors; glucocorticoids
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MDPI and ACS Style

Luostarinen, S.; Hämäläinen, M.; Moilanen, E. Transient Receptor Potential Ankyrin 1 (TRPA1)—An Inflammation-Induced Factor in Human HaCaT Keratinocytes. Int. J. Mol. Sci. 2021, 22, 3322. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22073322

AMA Style

Luostarinen S, Hämäläinen M, Moilanen E. Transient Receptor Potential Ankyrin 1 (TRPA1)—An Inflammation-Induced Factor in Human HaCaT Keratinocytes. International Journal of Molecular Sciences. 2021; 22(7):3322. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22073322

Chicago/Turabian Style

Luostarinen, Samu, Mari Hämäläinen, and Eeva Moilanen. 2021. "Transient Receptor Potential Ankyrin 1 (TRPA1)—An Inflammation-Induced Factor in Human HaCaT Keratinocytes" International Journal of Molecular Sciences 22, no. 7: 3322. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22073322

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