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Article

In Vivo Electrophysiology of Peptidergic Neurons in Deep Layers of the Lumbar Spinal Cord after Optogenetic Stimulation of Hypothalamic Paraventricular Oxytocin Neurons in Rats

by 1,*,†, 2,3,†, 3 and 3,*
1
Department of Applied Pharmacology, Faculty of Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan
2
Department of Biological Sciences, Faculty of Science, Kanagawa University, Hiratsuka, Kanagawa 259-1293, Japan
3
Ushimado Marine Institute (UMI), Graduate School of Natural Science and Technology, Okayama University, Ushimado, Setouchi, Okayama 701-4303, Japan
*
Authors to whom correspondence should be addressed.
Authors with equal contributions.
Academic Editor: Annamaria Morelli
Int. J. Mol. Sci. 2021, 22(7), 3400; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22073400
Received: 23 January 2021 / Revised: 22 March 2021 / Accepted: 23 March 2021 / Published: 26 March 2021
(This article belongs to the Special Issue Effects of Hormones on the Nervous System and Behavior)
The spinal ejaculation generator (SEG) is located in the central gray (lamina X) of the rat lumbar spinal cord and plays a pivotal role in the ejaculatory reflex. We recently reported that SEG neurons express the oxytocin receptor and are activated by oxytocin projections from the paraventricular nucleus of hypothalamus (PVH). However, it is unknown whether the SEG responds to oxytocin in vivo. In this study, we analyzed the characteristics of the brain–spinal cord neural circuit that controls male sexual function using a newly developed in vivo electrophysiological technique. Optogenetic stimulation of the PVH of rats expressing channel rhodopsin under the oxytocin receptor promoter increased the spontaneous firing of most lamina X SEG neurons. This is the first demonstration of the in vivo electrical response from the deeper (lamina X) neurons in the spinal cord. Furthermore, we succeeded in the in vivo whole-cell recordings of lamina X neurons. In vivo whole-cell recordings may reveal the features of lamina X SEG neurons, including differences in neurotransmitters and response to stimulation. Taken together, these results suggest that in vivo electrophysiological stimulation can elucidate the neurophysiological response of a variety of spinal neurons during male sexual behavior. View Full-Text
Keywords: in vivo extracellular recording; in vivo whole-cell patch-clamp recording; optogenetics; spinal cord; lamina X; spinal ejaculation generator; gastrin-releasing peptide neurons; oxytocin in vivo extracellular recording; in vivo whole-cell patch-clamp recording; optogenetics; spinal cord; lamina X; spinal ejaculation generator; gastrin-releasing peptide neurons; oxytocin
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MDPI and ACS Style

Uta, D.; Oti, T.; Sakamoto, T.; Sakamoto, H. In Vivo Electrophysiology of Peptidergic Neurons in Deep Layers of the Lumbar Spinal Cord after Optogenetic Stimulation of Hypothalamic Paraventricular Oxytocin Neurons in Rats. Int. J. Mol. Sci. 2021, 22, 3400. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22073400

AMA Style

Uta D, Oti T, Sakamoto T, Sakamoto H. In Vivo Electrophysiology of Peptidergic Neurons in Deep Layers of the Lumbar Spinal Cord after Optogenetic Stimulation of Hypothalamic Paraventricular Oxytocin Neurons in Rats. International Journal of Molecular Sciences. 2021; 22(7):3400. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22073400

Chicago/Turabian Style

Uta, Daisuke, Takumi Oti, Tatsuya Sakamoto, and Hirotaka Sakamoto. 2021. "In Vivo Electrophysiology of Peptidergic Neurons in Deep Layers of the Lumbar Spinal Cord after Optogenetic Stimulation of Hypothalamic Paraventricular Oxytocin Neurons in Rats" International Journal of Molecular Sciences 22, no. 7: 3400. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22073400

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