Sphk1 and Sphk2 Differentially Regulate Erythropoietin Synthesis in Mouse Renal Interstitial Fibroblast-like Cells
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Cell Lines and Cell Culture Conditions
4.3. Stable mRNA Knockdown in F3-5 and Hep3B Cells
4.4. Cell Stimulation, Homogenization, and Immunoblotting
4.5. RNA Extraction and Quantitative PCR Analysis
4.6. Sphingolipid Quantification by Mass Spectrometry
4.7. Red Blood Cell Counting
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
α-SMA | α-smooth muscle actin |
CKD | chronic kidney disease |
CTGF | connective tissue growth factor |
DMEM | Dulbecco’s modified Eagle medium |
Epo | erythropoietin |
FBS | fetal bovine serum |
GPCR | G protein-coupled receptor |
HIF | hypoxia-inducible factor |
kd | knockdown |
MAPK | mitogen-activated protein kinase |
PBS | phosphate-buffered saline |
PKC | protein kinase C |
qPCR | quantitative polymerase chain reaction |
REPC | renal Epo-producing cells |
S1P | sphingosine 1-phosphate |
SDS-PAGE | sodium dodecyl sulfate polyacrylamide gel electrophoresis |
shRNA | small hairpin RNA |
Sph | sphingosine |
SphK | sphingosine kinase |
UUO | unilateral ureteral obstruction |
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Hafizi, R.; Imeri, F.; Stepanovska Tanturovska, B.; Manaila, R.; Schwalm, S.; Trautmann, S.; Wenger, R.H.; Pfeilschifter, J.; Huwiler, A. Sphk1 and Sphk2 Differentially Regulate Erythropoietin Synthesis in Mouse Renal Interstitial Fibroblast-like Cells. Int. J. Mol. Sci. 2022, 23, 5882. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23115882
Hafizi R, Imeri F, Stepanovska Tanturovska B, Manaila R, Schwalm S, Trautmann S, Wenger RH, Pfeilschifter J, Huwiler A. Sphk1 and Sphk2 Differentially Regulate Erythropoietin Synthesis in Mouse Renal Interstitial Fibroblast-like Cells. International Journal of Molecular Sciences. 2022; 23(11):5882. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23115882
Chicago/Turabian StyleHafizi, Redona, Faik Imeri, Bisera Stepanovska Tanturovska, Roxana Manaila, Stephanie Schwalm, Sandra Trautmann, Roland H. Wenger, Josef Pfeilschifter, and Andrea Huwiler. 2022. "Sphk1 and Sphk2 Differentially Regulate Erythropoietin Synthesis in Mouse Renal Interstitial Fibroblast-like Cells" International Journal of Molecular Sciences 23, no. 11: 5882. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms23115882