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REV-ERBα Agonist SR9009 Promotes a Negative Energy Balance in Goldfish

Fish Neuroendocrinology Group, Department of Genetics, Physiology and Microbiology, Faculty of Biology, Complutense University of Madrid, 28040 Madrid, Spain
Centro de Investigación Mariña, Laboratorio de Fisioloxía Animal, Departamento de Bioloxía Funcional e Ciencias da Saúde, Facultade de Bioloxía, Universidade de Vigo, 36310 Vigo, Spain
Author to whom correspondence should be addressed.
Academic Editor: Carlo C. Lazado
Int. J. Mol. Sci. 2022, 23(6), 2921;
Received: 7 February 2022 / Revised: 2 March 2022 / Accepted: 3 March 2022 / Published: 8 March 2022
(This article belongs to the Special Issue Endocrine Control of Fish Metabolism)
REV-ERBα (nr1d1, nuclear receptor subfamily 1 group D member 1) is a transcriptional repressor that in mammals regulates nutrient metabolism, and has effects on energy homeostasis, although its role in teleosts is poorly understood. To determine REV-ERBα’s involvement in fish energy balance and metabolism, we studied the effects of acute and 7-day administration of its agonist SR9009 on food intake, weight and length gain, locomotor activity, feeding regulators, plasma and hepatic metabolites, and liver enzymatic activity. SR9009 inhibited feeding, lowering body weight and length gain. In addition, the abundance of ghrelin mRNA decreased in the intestine, and abundance of leptin-aI mRNA increased in the liver. Hypocretin, neuropeptide y (npy), and proopiomelanocortin (pomc) mRNA abundance was not modified after acute or subchronic SR9009 administration, while hypothalamic cocaine- and amphetamine-regulated transcript (cartpt-I) was induced in the subchronic treatment, being a possible mediator of the anorectic effects. Moreover, SR9009 decreased plasma glucose, coinciding with increased glycolysis and a decreased gluconeogenesis in the liver. Decreased triglyceride levels and activity of lipogenic enzymes suggest a lipogenesis reduction by SR9009. Energy expenditure by locomotor activity was not significantly affected by SR9009. Overall, this study shows for the first time in fish the effects of REV-ERBα activation via SR9009, promoting a negative energy balance by reducing energetic inputs and regulating lipid and glucose metabolism. View Full-Text
Keywords: fish; nr1d1; feeding; body weight; growth; metabolism; glucose; lipids fish; nr1d1; feeding; body weight; growth; metabolism; glucose; lipids
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MDPI and ACS Style

Saiz, N.; Herrera-Castillo, L.; Isorna, E.; Delgado, M.J.; Conde-Sieira, M.; Soengas, J.L.; de Pedro, N. REV-ERBα Agonist SR9009 Promotes a Negative Energy Balance in Goldfish. Int. J. Mol. Sci. 2022, 23, 2921.

AMA Style

Saiz N, Herrera-Castillo L, Isorna E, Delgado MJ, Conde-Sieira M, Soengas JL, de Pedro N. REV-ERBα Agonist SR9009 Promotes a Negative Energy Balance in Goldfish. International Journal of Molecular Sciences. 2022; 23(6):2921.

Chicago/Turabian Style

Saiz, Nuria, Lisbeth Herrera-Castillo, Esther Isorna, María J. Delgado, Marta Conde-Sieira, José L. Soengas, and Nuria de Pedro. 2022. "REV-ERBα Agonist SR9009 Promotes a Negative Energy Balance in Goldfish" International Journal of Molecular Sciences 23, no. 6: 2921.

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