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Identification and molecular modeling of a family 5 endocellulase from Thermus caldophilus GK24, a cellulolytic strain of Thermus thermophilus

1
Systems Microbiology Research Center, KRIBB, Daejeon 305-806, Korea
2
Department of Biomicrosystem Technology, Korea University, Seoul 136-701, Korea
3
Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, Korea
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2006, 7(12), 571-589; https://0-doi-org.brum.beds.ac.uk/10.3390/i7120571
Received: 20 November 2006 / Accepted: 14 December 2006 / Published: 18 December 2006
The genome of T. caldophilus GK24 was recently sequenced and annotated as 14contigs, equivalent to 2.3 mega basepairs (Mbp) of DNA. In the current study, we identifieda unique 13.7 kbp DNA sequence, which included the endocellulase gene of T. caldophilusGK24, which did not appear to be present in the complete genomic sequence of the closelyrelated species T. thermophilus HB27 and HB8. Congo-red staining revealed a uniquephenotype of cellulose degradation by strain GK24 that was distinct from other closelyrelated Thermus strains. The results showed that strain GK24 is an aerobic, thermophilic,cellulolytic eubacterium which belongs to the group T. thermophilus. In order to understandthe mechanism of production of cellobiose in T. caldophilus GK24, a three-dimensionalmodel of the endocellulase, TcCel5A, was generated based on known crystal structures.Using this model, we carried out a flexible cellotetraose docking study. View Full-Text
Keywords: Endocellulase; Thermus caldophilus GK24; Glycosyl hydrolase family 5; Homology modeling; Molecular dynamics; Congo-red staining Endocellulase; Thermus caldophilus GK24; Glycosyl hydrolase family 5; Homology modeling; Molecular dynamics; Congo-red staining
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MDPI and ACS Style

Kim, D.; Park, B.H.; Jung, B.-W.; Kim, M.-k.; Hong, S.-I.; Lee, D.-S. Identification and molecular modeling of a family 5 endocellulase from Thermus caldophilus GK24, a cellulolytic strain of Thermus thermophilus. Int. J. Mol. Sci. 2006, 7, 571-589. https://0-doi-org.brum.beds.ac.uk/10.3390/i7120571

AMA Style

Kim D, Park BH, Jung B-W, Kim M-k, Hong S-I, Lee D-S. Identification and molecular modeling of a family 5 endocellulase from Thermus caldophilus GK24, a cellulolytic strain of Thermus thermophilus. International Journal of Molecular Sciences. 2006; 7(12):571-589. https://0-doi-org.brum.beds.ac.uk/10.3390/i7120571

Chicago/Turabian Style

Kim, Dooil, Bo H. Park, Bo-Won Jung, Mi-kyung Kim, Suk-In Hong, and Dae-Sil Lee. 2006. "Identification and molecular modeling of a family 5 endocellulase from Thermus caldophilus GK24, a cellulolytic strain of Thermus thermophilus" International Journal of Molecular Sciences 7, no. 12: 571-589. https://0-doi-org.brum.beds.ac.uk/10.3390/i7120571

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