Next Article in Journal
A Quest for the Origin of Barrier to the Internal Rotation ofHydrogen Peroxide (H2O2) and Fluorine Peroxide (F2O2)
Previous Article in Journal
Determination of Trace Level of cAMP in Locusta Migratoria Manilensis Meyen by HPLC with Fluorescence Derivation
Article

Enzyme Chemiluminescence Immuno Assay of Free hCGβ in Serum Based on Superparamagnetic Polymer Microbeads

1
State Key Bioelectronics Laboratory, Southeast University, Sipailou2#, Nanjing, 210096, P.R. China
2
Institute of Chemistry & Hydrometallurgy, Jinchuan Group Co., Ltd., Lanzhou Road, Jinchang,737100, P.R.China
3
Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering,Chinese Academy of Sciences, Beijing, 100080, P.R. China
4
Technical Institute of Physics and Chemistry, Chinese Academy of Science, Datun Street First 3#, Beijing, 100101, P.R. China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2006, 7(8), 274-288; https://0-doi-org.brum.beds.ac.uk/10.3390/i7080274
Received: 26 May 2006 / Accepted: 3 August 2006 / Published: 24 August 2006
Superparamagnetic polymers (SPMP) microbead was an excellent form fornanosized magnetic particles less than 10nm to realize their potential applications in manyfields. We developed a novel modified suspension polymerization method for the productionof superparamagnetic poly (methacrylate divinylbenzene) (PMA-DVB) microbeads, withamino groups on their surface after simple modifications. We applied these SPMPmicrobeads to establish a sandwich enzyme chemiluminence immuno (ECLIA) procedure ofdetecting the free hCGβ in serum. It was proved to a better method compared to the ELISA,since it need half of the sample volume, simpler protocols, and the time it need wasshortened from 2 hours to 1 hour. The detection limit was 0.22 mIU•m-1, one order lowerthan the ELISA assay. Its linear range was between 0.45—185.2 mIU•m-1. And in thedetections of clinical serum samples, the related coefficient between the two methods’results was 0.955. View Full-Text
Keywords: superparamagnetic polymer microbeads; modified suspension polymerization; enzyme chemiluminescence immunoassay; free hCGβ; tumor marker. superparamagnetic polymer microbeads; modified suspension polymerization; enzyme chemiluminescence immunoassay; free hCGβ; tumor marker.
Show Figures

Figure 1

MDPI and ACS Style

Guo, X.; Guan, Y.; Yang, B.; Wang, Y.; Lan, H.; Shi, W.; Yang, Z.; Lu, Z. Enzyme Chemiluminescence Immuno Assay of Free hCGβ in Serum Based on Superparamagnetic Polymer Microbeads. Int. J. Mol. Sci. 2006, 7, 274-288. https://0-doi-org.brum.beds.ac.uk/10.3390/i7080274

AMA Style

Guo X, Guan Y, Yang B, Wang Y, Lan H, Shi W, Yang Z, Lu Z. Enzyme Chemiluminescence Immuno Assay of Free hCGβ in Serum Based on Superparamagnetic Polymer Microbeads. International Journal of Molecular Sciences. 2006; 7(8):274-288. https://0-doi-org.brum.beds.ac.uk/10.3390/i7080274

Chicago/Turabian Style

Guo, Xiaoying, Yueping Guan, Bin Yang, Yongning Wang, Hualong Lan, Wentang Shi, Zhenghui Yang, and Zuhong Lu. 2006. "Enzyme Chemiluminescence Immuno Assay of Free hCGβ in Serum Based on Superparamagnetic Polymer Microbeads" International Journal of Molecular Sciences 7, no. 8: 274-288. https://0-doi-org.brum.beds.ac.uk/10.3390/i7080274

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop