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Sensors, Volume 1, Issue 2 (July 2001) – 2 articles , Pages 53-74

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143 KiB  
Article
New Trends in Biosensors for Organophosphorus Pesticides
by Nicole Jaffrezic-Renault
Sensors 2001, 1(2), 60-74; https://0-doi-org.brum.beds.ac.uk/10.3390/s10100060 - 24 Jul 2001
Cited by 118 | Viewed by 11846
Abstract
Biosensors are sensitive and can be used as disposable sensors for environmental control. These biosensors are based either on inhibition of acyl cholinesterases acetylcholinesterase or butyrylcholinesterase) by organophosphorus compounds or on inhibition of enzymes phosphatases (acid or alkaline) or on direct detection of [...] Read more.
Biosensors are sensitive and can be used as disposable sensors for environmental control. These biosensors are based either on inhibition of acyl cholinesterases acetylcholinesterase or butyrylcholinesterase) by organophosphorus compounds or on inhibition of enzymes phosphatases (acid or alkaline) or on direct detection of organophosphorus compounds by organophosphorus hydrolase. The state-of-the-art of the three types of biosensors will be presented in this paper and a particular development will be done on our work on ENFET (Enzymatic Field Effect Transistor) biosensors. Full article
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110 KiB  
Article
Assembly method fabricating linkers for covalently bonding DNA on glass surface
by Jiong Li, Hong Wang, Yujie Zhao, Lu Cheng, Nongyue He and Zuhong Lu
Sensors 2001, 1(2), 53-59; https://0-doi-org.brum.beds.ac.uk/10.3390/s10100053 - 20 Jul 2001
Cited by 19 | Viewed by 9186
Abstract
A fabrication method of linkers to covalently anchor nucleic acid probes (such as, oligonucleotides, PCR-products or peptide nucleic acid oligomers) on glass was developed by alternatively covalently assembling the different molecular components. The linkers with controllable length, flexibility and hydrophilicity could be prepared [...] Read more.
A fabrication method of linkers to covalently anchor nucleic acid probes (such as, oligonucleotides, PCR-products or peptide nucleic acid oligomers) on glass was developed by alternatively covalently assembling the different molecular components. The linkers with controllable length, flexibility and hydrophilicity could be prepared simply by dipping subtracts alternatively in different chemical solutions. A dialkoxy aminosilane (N-(2-aminoethyl)-3-aminopropylmethyl dimethoxylsilane, AEAPS) was chosen to substitute common used trialkoxy aminosilane to modify glass surface. The end groups of the linkers were formed as aldehyde or amino group, which were successfully used for attaching prefabricated DNA or in situ synthesis of oligonucleotides, respectively. The both experiments showed that the linker produce good reproducibility and uniformity of fluorescent hybridizing images, which can distinguish an internal single base mismatch in 20mer oligonucleotides. Full article
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