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Article

Antigiardial Activity of Acetylsalicylic Acid Is Associated with Overexpression of HSP70 and Membrane Transporters

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Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Col. Independencia, Guadalajara, Jalisco 44340, Mexico
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Departamento de Biología Celular, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional, Av. Instituto Politécnico Nacional 2508, Col. San Pedro Zacatenco, Ciudad de México 07360, Mexico
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Departamento de Microbiología y Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Col. Independencia, Guadalajara, Jalisco 44340, Mexico
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Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Circuito Exterior s/n Ciudad Universitaria, Coyoacán, Ciudad de México 04510, Mexico
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Departamento de Zoología, Escuela Nacional de Ciencias Biológicas, IPN, Carpio y Plan de Ayala S/N, Casco de Santo Tomás, Ciudad de México 11340, Mexico
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Unidad de Microscopía Electrónica LaNSE, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional, Av. Instituto Politécnico Nacional 2508, Col. San Pedro Zacatenco, Ciudad de México 07360, Mexico
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Author to whom correspondence should be addressed.
Pharmaceuticals 2020, 13(12), 440; https://0-doi-org.brum.beds.ac.uk/10.3390/ph13120440
Received: 9 October 2020 / Revised: 29 November 2020 / Accepted: 30 November 2020 / Published: 3 December 2020
(This article belongs to the Special Issue Antiparasitics)
Giardia lamblia is a flagellated protozoan responsible for giardiasis, a worldwide diarrheal disease. The adverse effects of the pharmacological treatments and the appearance of drug resistance have increased the rate of therapeutic failures. In the search for alternative therapeutics, drug repositioning has become a popular strategy. Acetylsalicylic acid (ASA) exhibits diverse biological activities through multiple mechanisms. However, the full spectrum of its activities is incompletely understood. In this study we show that ASA displayed direct antigiardial activity and affected the adhesion and growth of trophozoites in a time-dose-dependent manner. Electron microscopy images revealed remarkable morphological alterations in the membrane, ventral disk, and caudal region. Using mass spectrometry and real-time quantitative reverse transcription (qRT-PCR), we identified that ASA induced the overexpression of heat shock protein 70 (HSP70). ASA also showed a significant increase of five ATP-binding cassette (ABC) transporters (giABC, giABCP, giMDRP, giMRPL and giMDRAP1). Additionally, we found low toxicity on Caco-2 cells. Taken together, these results suggest an important role of HSPs and ABC drug transporters in contributing to stress tolerance and protecting cells from ASA-induced stress. View Full-Text
Keywords: Giardia lamblia; acetylsalicylic acid; heat shock protein 70; membrane transporter Giardia lamblia; acetylsalicylic acid; heat shock protein 70; membrane transporter
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MDPI and ACS Style

Ochoa-Maganda, V.Y.; Rangel-Castañeda, I.A.; Suárez-Rico, D.O.; Cortés-Zárate, R.; Hernández-Hernández, J.M.; Pérez-Rangel, A.; Chiquete-Félix, N.; León-Ávila, G.; González-Pozos, S.; Gaona-Bernal, J.; Castillo-Romero, A. Antigiardial Activity of Acetylsalicylic Acid Is Associated with Overexpression of HSP70 and Membrane Transporters. Pharmaceuticals 2020, 13, 440. https://0-doi-org.brum.beds.ac.uk/10.3390/ph13120440

AMA Style

Ochoa-Maganda VY, Rangel-Castañeda IA, Suárez-Rico DO, Cortés-Zárate R, Hernández-Hernández JM, Pérez-Rangel A, Chiquete-Félix N, León-Ávila G, González-Pozos S, Gaona-Bernal J, Castillo-Romero A. Antigiardial Activity of Acetylsalicylic Acid Is Associated with Overexpression of HSP70 and Membrane Transporters. Pharmaceuticals. 2020; 13(12):440. https://0-doi-org.brum.beds.ac.uk/10.3390/ph13120440

Chicago/Turabian Style

Ochoa-Maganda, Verónica Y., Itzia A. Rangel-Castañeda, Daniel O. Suárez-Rico, Rafael Cortés-Zárate, José M. Hernández-Hernández, Armando Pérez-Rangel, Natalia Chiquete-Félix, Gloria León-Ávila, Sirenia González-Pozos, Jorge Gaona-Bernal, and Araceli Castillo-Romero. 2020. "Antigiardial Activity of Acetylsalicylic Acid Is Associated with Overexpression of HSP70 and Membrane Transporters" Pharmaceuticals 13, no. 12: 440. https://0-doi-org.brum.beds.ac.uk/10.3390/ph13120440

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