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Article

The In Vitro Anti-amyloidogenic Activity of the Mediterranean Red Seaweed Halopithys Incurva

1
Department of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, Italy
2
Institute for Complex Systems-National Research Council (ISC-CNR), Via Madonna del piano 10, 50019 Sesto Fiorentino, Florence, Italy
3
Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy
4
Interuniversity Center of Marine Biology and Applied Ecology “G. Bacci” (CIBM), Viale N. Sauro 4, 57128 Livorno, Italy
5
Department of NEUROFARBA, Nutraceutical section, University of Florence, Via Ugo Schiff 6, 50019 Sesto Fiorentino, Italy
*
Author to whom correspondence should be addressed.
These authors equally contribute to the work.
Pharmaceuticals 2020, 13(8), 185; https://0-doi-org.brum.beds.ac.uk/10.3390/ph13080185
Received: 6 July 2020 / Revised: 30 July 2020 / Accepted: 5 August 2020 / Published: 7 August 2020
(This article belongs to the Special Issue Medicinal Plants 2020)
Neurodegenerative diseases are generally characterized by the presence of neurotoxic amyloid aggregates underlying progressive neuronal death. Since ancient times, natural compounds have been used as curative agents for human health. Amyloid research is constantly looking for safe natural molecules capable of blocking toxic amyloid aggregates’ formation. From the marine environment, seaweeds are recognized as rich reservoirs of molecules with multiple bioactivities, including the anti-amyloidogenic activity. Here, hydroalcoholic extracts of two seasonal samples of the Mediterranean red seaweed Halophytis incurva (HIEs) were characterized by the HPLC-DAD-MS analysis. The H. incurva anti-amyloidogenic role was explored by incubating both HIEs with hen egg white lysozyme (HEWL), a well-known protein model widely used in amyloid aggregation experiments. The aggregation kinetics and morphological analysis of amyloid aggregates were performed by ThT and AFM analysis, respectively, while their cytotoxicity on SH-SY5Y human neuroblastoma cells was examined by MTT assay. HIEs showed a different efficacy, probably dependent on their metabolic composition, both in inhibiting amyloid fibrillation and in obtaining short and less toxic pre-fibrillary aggregates. Overall, this work sheds light, for the first time, on a Mediterranean red seaweed as a promising renewable resource of bioactive compounds, potentially useful in preventing the formation of toxic amyloid aggregates. View Full-Text
Keywords: Halophytis incurva; red seaweed; amyloid aggregation; HEWL; anti-amyloidogenic Halophytis incurva; red seaweed; amyloid aggregation; HEWL; anti-amyloidogenic
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MDPI and ACS Style

Vasarri, M.; Ramazzotti, M.; Tiribilli, B.; Barletta, E.; Pretti, C.; Mulinacci, N.; Degl’Innocenti, D. The In Vitro Anti-amyloidogenic Activity of the Mediterranean Red Seaweed Halopithys Incurva. Pharmaceuticals 2020, 13, 185. https://0-doi-org.brum.beds.ac.uk/10.3390/ph13080185

AMA Style

Vasarri M, Ramazzotti M, Tiribilli B, Barletta E, Pretti C, Mulinacci N, Degl’Innocenti D. The In Vitro Anti-amyloidogenic Activity of the Mediterranean Red Seaweed Halopithys Incurva. Pharmaceuticals. 2020; 13(8):185. https://0-doi-org.brum.beds.ac.uk/10.3390/ph13080185

Chicago/Turabian Style

Vasarri, Marzia, Matteo Ramazzotti, Bruno Tiribilli, Emanuela Barletta, Carlo Pretti, Nadia Mulinacci, and Donatella Degl’Innocenti. 2020. "The In Vitro Anti-amyloidogenic Activity of the Mediterranean Red Seaweed Halopithys Incurva" Pharmaceuticals 13, no. 8: 185. https://0-doi-org.brum.beds.ac.uk/10.3390/ph13080185

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