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Article

Annurca Apple Polyphenol Extract Affects Acetyl- Cholinesterase and Mono-Amine Oxidase In Vitro Enzyme Activity

1
Dipartimento di Scienze Motorie e del Benessere, Università degli Studi di Napoli “Parthenope”, Via Medina 40, 80133 Napoli, Italy
2
ISPAAM, Consiglio Nazionale delle Ricerche, Piazzale Enrico Fermi 1, 80055 Portici, Italy
3
CEINGE, Biotecnologie Avanzate, S.C. a R.L., Via G. Salvatore 486, 80145 Napoli, Italy
*
Authors to whom correspondence should be addressed.
Rosarita Nasso and Valentina Pagliara equally contributed to this work.
Pharmaceuticals 2021, 14(1), 62; https://0-doi-org.brum.beds.ac.uk/10.3390/ph14010062
Received: 1 December 2020 / Revised: 8 January 2021 / Accepted: 11 January 2021 / Published: 14 January 2021
In this study, we explored the ability of Annurca apple flesh polyphenol extract (AFPE) to affect the activity of key enzymes involved in neurodegenerative disorders—in particular, Acetyl- and Butirryl-cholinesterases, and type A and B monoamine oxidase. The effect of AFPE on enzyme activity was analyzed by in vitro enzyme assays, and the results showed concentration-dependent enzyme inhibition, with IC50 values corresponding to 859 ± 18 µM and 966 ± 72 µM for AChE and BuChE respectively, and IC50 corresponding to 145 ± 3 µM and 199 ± 7 µM for MAO-A and MAO-B, respectively, with a preference for MAO-A. Moreover, in this concentration range, AFPE did not affect the viability of human neuroblastoma SH-SY5Y and fibroblast BJ-5ta cell lines, as determined by an MTT assay. In conclusion, our results demonstrate that AFPE shows the new biological properties of inhibiting the activity of enzymes that are involved in brain functions, neurodegenerative disorders, and aging. View Full-Text
Keywords: Annurca apple polyphenols; cholinesterase activity; monoamine oxidase activity; neuroblastoma cells; cell viability Annurca apple polyphenols; cholinesterase activity; monoamine oxidase activity; neuroblastoma cells; cell viability
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MDPI and ACS Style

Nasso, R.; Pagliara, V.; D’Angelo, S.; Rullo, R.; Masullo, M.; Arcone, R. Annurca Apple Polyphenol Extract Affects Acetyl- Cholinesterase and Mono-Amine Oxidase In Vitro Enzyme Activity. Pharmaceuticals 2021, 14, 62. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14010062

AMA Style

Nasso R, Pagliara V, D’Angelo S, Rullo R, Masullo M, Arcone R. Annurca Apple Polyphenol Extract Affects Acetyl- Cholinesterase and Mono-Amine Oxidase In Vitro Enzyme Activity. Pharmaceuticals. 2021; 14(1):62. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14010062

Chicago/Turabian Style

Nasso, Rosarita, Valentina Pagliara, Stefania D’Angelo, Rosario Rullo, Mariorosario Masullo, and Rosaria Arcone. 2021. "Annurca Apple Polyphenol Extract Affects Acetyl- Cholinesterase and Mono-Amine Oxidase In Vitro Enzyme Activity" Pharmaceuticals 14, no. 1: 62. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14010062

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