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Article

Metabolite Profiling of Methanolic Extract of Gardenia jaminoides by LC-MS/MS and GC-MS and Its Anti-Diabetic, and Anti-Oxidant Activities

1
Department of Bio-Health Convergence, Kangwon National University, Chuncheon 24341, Korea
2
Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon 24341, Korea
3
Department of Medical Biomaterials Engineering, College of Biomedical Sciences, Kangwon National University, Chuncheon 24341, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally.
Academic Editors: Sabina Lachowicz and Jan Oszmianski
Pharmaceuticals 2021, 14(2), 102; https://0-doi-org.brum.beds.ac.uk/10.3390/ph14020102
Received: 31 December 2020 / Revised: 20 January 2021 / Accepted: 25 January 2021 / Published: 28 January 2021
In this study, the methanolic extract from seeds of Gardenia jasminoides exhibited strong antioxidant and enzyme inhibition activities with less toxicity to NIH3T3 and HepG2 cells at the concentration of 100 µg/mL. The antioxidant activities (DPPH and ABTS), α-amylase, and α-glucosidase inhibition activities were found higher in methanolic extract (MeOH-E) than H2O extract. Besides, 9.82 ± 0.62 µg and 6.42 ± 0.26 µg of MeOH-E were equivalent to 1 µg ascorbic acid for ABTS and DPPH scavenging, respectively while 9.02 ± 0.25 µg and 6.52 ± 0.15 µg of MeOH-E were equivalent to 1 µg of acarbose for inhibition of α-amylase and α-glucosidase respectively. Moreover, the cell assay revealed that the addition of MeOH-E (12.5 µg/mL) increased about 37% of glucose uptake in insulin resistant (IR) HepG2 as compared to untreated IR HepG2 cells. The LC- MS/MS and GC-MS analysis of MeOH-E revealed a total of 54 compounds including terpenoids, glycosides, fatty acid, phenolic acid derivatives. Among the identified compounds, chlorogenic acid and jasminoside A were found promising for anti-diabetic activity revealed by molecular docking study and these molecules are deserving further purification and molecular analysis. View Full-Text
Keywords: Gardenia jasminoides Ellis; anti-diabetic activity; LC-MS/MS; GC-MS; anti-oxidant Gardenia jasminoides Ellis; anti-diabetic activity; LC-MS/MS; GC-MS; anti-oxidant
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MDPI and ACS Style

Saravanakumar, K.; Park, S.; Sathiyaseelan, A.; Kim, K.-N.; Cho, S.-H.; Mariadoss, A.V.A.; Wang, M.-H. Metabolite Profiling of Methanolic Extract of Gardenia jaminoides by LC-MS/MS and GC-MS and Its Anti-Diabetic, and Anti-Oxidant Activities. Pharmaceuticals 2021, 14, 102. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14020102

AMA Style

Saravanakumar K, Park S, Sathiyaseelan A, Kim K-N, Cho S-H, Mariadoss AVA, Wang M-H. Metabolite Profiling of Methanolic Extract of Gardenia jaminoides by LC-MS/MS and GC-MS and Its Anti-Diabetic, and Anti-Oxidant Activities. Pharmaceuticals. 2021; 14(2):102. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14020102

Chicago/Turabian Style

Saravanakumar, Kandasamy, SeonJu Park, Anbazhagan Sathiyaseelan, Kil-Nam Kim, Su-Hyeon Cho, Arokia V.A. Mariadoss, and Myeong-Hyeon Wang. 2021. "Metabolite Profiling of Methanolic Extract of Gardenia jaminoides by LC-MS/MS and GC-MS and Its Anti-Diabetic, and Anti-Oxidant Activities" Pharmaceuticals 14, no. 2: 102. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14020102

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