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Article

Evaluation of the Interactions between Human Serum Albumin (HSA) and Non-Steroidal Anti-Inflammatory (NSAIDs) Drugs by Multiwavelength Molecular Fluorescence, Structural and Computational Analysis

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Department of Chemical Engineering and Analytical Chemistry, Faculty of Chemistry, University of Barcelona, Martí i Franquès 1-11, 08028 Barcelona, Spain
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Institute of Biomedicine (IBUB), University of Barcelona, 08028 Barcelona, Spain
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Department of Nutrition, Food Science and Gastronomy, Faculty of Pharmacy and Food Science, University of Barcelona, Prat de la Riba 171, 08921 Santa Coloma de Gramenet, Spain
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Pharmacelera, Torre R, 4a planta, Despatx A05, Parc Científic de Barcelona, Baldiri Reixac 8, 08028 Barcelona, Spain
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Institut of Theoretical and Computational Chemistry (IQTCUB), University of Barcelona, 08028 Barcelona, Spain
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Author to whom correspondence should be addressed.
Academic Editor: Osvaldo Andrade Santos-Filho
Pharmaceuticals 2021, 14(3), 214; https://0-doi-org.brum.beds.ac.uk/10.3390/ph14030214
Received: 2 February 2021 / Revised: 26 February 2021 / Accepted: 26 February 2021 / Published: 4 March 2021
(This article belongs to the Section Medicinal Chemistry)
The interaction between drugs and transport proteins, such as albumins, is a key factor in drug bioavailability. One of the techniques commonly used for the evaluation of the drug-protein complex formation is fluorescence. This work studies the interaction of human serum albumin (HSA) with four non-steroidal anti-inflammatory drugs (NSAIDs)—ibuprofen, flurbiprofen, naproxen, and diflunisal—by monitoring the fluorescence quenching when the drug-albumin complex is formed. Two approaches—the double logarithm Stern-Volmer equation and the STAR program—are used to evaluate the binding parameters. The results are analyzed considering the binding properties, determined by using other complementary techniques and the available structural information of albumin complexes with NSAID-related compounds. Finally, this combined analysis has been synergistically used to interpret the binding of flurbiprofen to HSA. View Full-Text
Keywords: non-steroidal anti-inflammatory drugs; drug-protein interactions; human serum albumin; fluorescence multiwavelength data treatment; fluorescence quenching; molecular modeling non-steroidal anti-inflammatory drugs; drug-protein interactions; human serum albumin; fluorescence multiwavelength data treatment; fluorescence quenching; molecular modeling
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MDPI and ACS Style

Amézqueta, S.; Beltrán, J.L.; Bolioli, A.M.; Campos-Vicens, L.; Luque, F.J.; Ràfols, C. Evaluation of the Interactions between Human Serum Albumin (HSA) and Non-Steroidal Anti-Inflammatory (NSAIDs) Drugs by Multiwavelength Molecular Fluorescence, Structural and Computational Analysis. Pharmaceuticals 2021, 14, 214. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14030214

AMA Style

Amézqueta S, Beltrán JL, Bolioli AM, Campos-Vicens L, Luque FJ, Ràfols C. Evaluation of the Interactions between Human Serum Albumin (HSA) and Non-Steroidal Anti-Inflammatory (NSAIDs) Drugs by Multiwavelength Molecular Fluorescence, Structural and Computational Analysis. Pharmaceuticals. 2021; 14(3):214. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14030214

Chicago/Turabian Style

Amézqueta, Susana, José L. Beltrán, Anna M. Bolioli, Lluís Campos-Vicens, Francisco J. Luque, and Clara Ràfols. 2021. "Evaluation of the Interactions between Human Serum Albumin (HSA) and Non-Steroidal Anti-Inflammatory (NSAIDs) Drugs by Multiwavelength Molecular Fluorescence, Structural and Computational Analysis" Pharmaceuticals 14, no. 3: 214. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14030214

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