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Article

Comprehensive and Rapid Quality Evaluation Method for the Ayurvedic Medicine Divya-Swasari-Vati Using Two Analytical Techniques: UPLC/QToF MS and HPLC–DAD

1
Drug Discovery and Development Division, Patanjali Research Institute, Haridwar 249 405, Uttarakhand, India
2
Department of Allied and Applied Sciences, University of Patanjali, Haridwar 249 405, Uttarakhand, India
*
Author to whom correspondence should be addressed.
Academic Editors: Thomas Efferth, Jan Oszmianski and Sabina Lachowicz
Pharmaceuticals 2021, 14(4), 297; https://0-doi-org.brum.beds.ac.uk/10.3390/ph14040297
Received: 12 February 2021 / Revised: 5 March 2021 / Accepted: 13 March 2021 / Published: 27 March 2021
Divya-Swasari-Vati (DSV) is a calcium-containing herbal medicine formulated for the symptomatic control of respiratory illnesses observed in the current COVID-19 pandemic. DSV is an Ayurvedic medicine used for the treatment of chronic cough and inflammation. The formulation has shown its pharmacological effects against SARS-CoV-2 induced inflammation in the humanized zebrafish model. The present inventive research aimed to establish comprehensive quality parameters of the DSV formulation using validated chromatographic analytical tools. Exhaustive identification of signature marker compounds present in the plant ingredients was carried out using ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/QToF MS). This was followed by simultaneous estimation of selected marker components using rapid and reliable high-performance liquid chromatography (HPLC) analysis. Eleven marker components, namely gallic acid, protocatechuic acid, methyl gallate, ellagic acid, coumarin, cinnamic acid, glycyrrhizin, eugenol, 6-gingerol, piperine and glabridin, were selected out of seventy-four identified makers for the quantitative analysis in DSV formulation. Validation of the HPLC method was evaluated by its linearity, precision, and accuracy tests as per the International Council of Harmonization (ICH) guidelines. Calibration curves for the eleven marker compounds showed good linear regression (r2 > 0.999). The relative standard deviation (RSD) value of intraday and interday precision tests were within the prescribed limits. The accuracy test results ranged from 92.75% to 100.13%. Thus, the present inclusive approach is first of its kind employing multi-chromatographic platforms for identification and quantification of the marker components in DSV, which could be applied for routine standardization of DSV and other related formulations. View Full-Text
Keywords: Ayurveda; Divya-Swasari-Vati; herbal medicine; UPLC/QToF MS; HPLC; validation Ayurveda; Divya-Swasari-Vati; herbal medicine; UPLC/QToF MS; HPLC; validation
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MDPI and ACS Style

Balkrishna, A.; Verma, S.; Sharma, P.; Tomer, M.; Srivastava, J.; Varshney, A. Comprehensive and Rapid Quality Evaluation Method for the Ayurvedic Medicine Divya-Swasari-Vati Using Two Analytical Techniques: UPLC/QToF MS and HPLC–DAD. Pharmaceuticals 2021, 14, 297. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14040297

AMA Style

Balkrishna A, Verma S, Sharma P, Tomer M, Srivastava J, Varshney A. Comprehensive and Rapid Quality Evaluation Method for the Ayurvedic Medicine Divya-Swasari-Vati Using Two Analytical Techniques: UPLC/QToF MS and HPLC–DAD. Pharmaceuticals. 2021; 14(4):297. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14040297

Chicago/Turabian Style

Balkrishna, Acharya, Sudeep Verma, Priyanka Sharma, Meenu Tomer, Jyotish Srivastava, and Anurag Varshney. 2021. "Comprehensive and Rapid Quality Evaluation Method for the Ayurvedic Medicine Divya-Swasari-Vati Using Two Analytical Techniques: UPLC/QToF MS and HPLC–DAD" Pharmaceuticals 14, no. 4: 297. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14040297

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