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Article

Wound Healing Promotion by Hyaluronic Acid: Effect of Molecular Weight on Gene Expression and In Vivo Wound Closure

1
Laboratory of Pre-Formulation Study, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba 278-8510, Japan
2
Institute of Pharmaceutical Sciences of Western Switzerland, University of Geneva, 1 Rue Michel Servet, 1211 Geneva, Switzerland
3
Section of Pharmaceutical Sciences, University of Geneva, 1 Rue Michel Servet, 1211 Geneva, Switzerland
4
Faculty of Pharmaceutical Sciences, Sanyo-Onoda City University, Yamaguchi 756-0884, Japan
5
Kewpie Corporation, Tokyo 182-0002, Japan
*
Authors to whom correspondence should be addressed.
Co-first authors.
Academic Editor: María Ángeles Peña Fernández
Pharmaceuticals 2021, 14(4), 301; https://0-doi-org.brum.beds.ac.uk/10.3390/ph14040301
Received: 2 March 2021 / Revised: 21 March 2021 / Accepted: 24 March 2021 / Published: 28 March 2021
(This article belongs to the Special Issue Formulations for Wound Healing)
Hyaluronic acid (HA) has been known to play an important role in wound healing process. However, the effect of molecular weight (MW) of exogenously administered HA on the wound healing process has not been fully understood. In this study, we investigated HA with different MWs on wound healing process using human epidermal keratinocytes and dermal fibroblasts. Cell proliferation and migration ability were assessed by water soluble tetrazolium (WST) assay and wound scratch assay. We examined the effect of HA addition in a full-thickness wound model in mice and the gene expression related to wound healing. Proliferation and migration of HaCaT cells increased with the increase of MW and concentration of HA. Interleukin (IL-1β), IL-8 and vascular endothelial growth factor (VEGF) as well as matrix metalloproteinase (MMP)-9 and MMP-13 were significantly upregulated by high molecular weight (HMW) HA in keratinocytes. Together with VEGF upregulation and the observed promotion of HaCaT migration, HA with the MW of 2290 kDa may hold potential to improve re-epithelialization, a critical obstacle to heal chronic wounds. View Full-Text
Keywords: hyaluronic acid; accelerated wound healing; epidermal cells; cytokines hyaluronic acid; accelerated wound healing; epidermal cells; cytokines
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MDPI and ACS Style

Kawano, Y.; Patrulea, V.; Sublet, E.; Borchard, G.; Iyoda, T.; Kageyama, R.; Morita, A.; Seino, S.; Yoshida, H.; Jordan, O.; Hanawa, T. Wound Healing Promotion by Hyaluronic Acid: Effect of Molecular Weight on Gene Expression and In Vivo Wound Closure. Pharmaceuticals 2021, 14, 301. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14040301

AMA Style

Kawano Y, Patrulea V, Sublet E, Borchard G, Iyoda T, Kageyama R, Morita A, Seino S, Yoshida H, Jordan O, Hanawa T. Wound Healing Promotion by Hyaluronic Acid: Effect of Molecular Weight on Gene Expression and In Vivo Wound Closure. Pharmaceuticals. 2021; 14(4):301. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14040301

Chicago/Turabian Style

Kawano, Yayoi, Viorica Patrulea, Emmanuelle Sublet, Gerrit Borchard, Takuya Iyoda, Rihoko Kageyama, Asa Morita, Satoshi Seino, Hideto Yoshida, Olivier Jordan, and Takehisa Hanawa. 2021. "Wound Healing Promotion by Hyaluronic Acid: Effect of Molecular Weight on Gene Expression and In Vivo Wound Closure" Pharmaceuticals 14, no. 4: 301. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14040301

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