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Review

Transgene Delivery to Human Induced Pluripotent Stem Cells Using Nanoparticles

1
Department of Economics, University of Oxford, Oxford OX1 3UQ, UK
2
Department of Internal Medicine, School of Medicine, University of California, Davis, CA 95616, USA
3
Department of Veterans Affairs, Northern California Health Care System, Mather, CA 95655, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Dimitris Tsiourvas
Pharmaceuticals 2021, 14(4), 334; https://0-doi-org.brum.beds.ac.uk/10.3390/ph14040334
Received: 6 March 2021 / Revised: 25 March 2021 / Accepted: 2 April 2021 / Published: 6 April 2021
Human induced pluripotent stem cells (hiPSCs) and hiPSCs-derived cells have the potential to revolutionize regenerative and precision medicine. Genetically reprograming somatic cells to generate hiPSCs and genetic modification of hiPSCs are considered the key procedures for the study and application of hiPSCs. However, there are significant technical challenges for transgene delivery into somatic cells and hiPSCs since these cells are known to be difficult to transfect. The existing methods, such as viral transduction and chemical transfection, may introduce significant alternations to hiPSC culture which affect the potency, purity, consistency, safety, and functional capacity of hiPSCs. Therefore, generation and genetic modification of hiPSCs through non-viral approaches are necessary and desirable. Nanotechnology has revolutionized fields from astrophysics to biology over the past two decades. Increasingly, nanoparticles have been used in biomedicine as powerful tools for transgene and drug delivery, imaging, diagnostics, and therapeutics. The most successful example is the recent development of SARS-CoV-2 vaccines at warp speed to combat the 2019 coronavirus disease (COVID-19), which brought nanoparticles to the center stage of biomedicine and demonstrated the efficient nanoparticle-mediated transgene delivery into human body. Nanoparticles have the potential to facilitate the transgene delivery into the hiPSCs and offer a simple and robust approach. Nanoparticle-mediated transgene delivery has significant advantages over other methods, such as high efficiency, low cytotoxicity, biodegradability, low cost, directional and distal controllability, efficient in vivo applications, and lack of immune responses. Our recent study using magnetic nanoparticles for transfection of hiPSCs provided an example of the successful applications, supporting the potential roles of nanoparticles in hiPSC biology. This review discusses the principle, applications, and significance of nanoparticles in the transgene delivery to hiPSCs and their successful application in the development of COVID-19 vaccines. View Full-Text
Keywords: nanoparticles; magnetic nanoparticles; human induced pluripotent stem cells; human induced pluripotent stem cells-derived cardiomyocytes; transgene; transfection; nanomedicine; SARS-CoV-2; COVID-19; vaccine nanoparticles; magnetic nanoparticles; human induced pluripotent stem cells; human induced pluripotent stem cells-derived cardiomyocytes; transgene; transfection; nanomedicine; SARS-CoV-2; COVID-19; vaccine
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MDPI and ACS Style

Yamoah, M.A.; Thai, P.N.; Zhang, X.-D. Transgene Delivery to Human Induced Pluripotent Stem Cells Using Nanoparticles. Pharmaceuticals 2021, 14, 334. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14040334

AMA Style

Yamoah MA, Thai PN, Zhang X-D. Transgene Delivery to Human Induced Pluripotent Stem Cells Using Nanoparticles. Pharmaceuticals. 2021; 14(4):334. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14040334

Chicago/Turabian Style

Yamoah, Megan A., Phung N. Thai, and Xiao-Dong Zhang. 2021. "Transgene Delivery to Human Induced Pluripotent Stem Cells Using Nanoparticles" Pharmaceuticals 14, no. 4: 334. https://0-doi-org.brum.beds.ac.uk/10.3390/ph14040334

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