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Optimization of Hydrolysis Conditions for the Production of Angiotensin-I Converting Enzyme-Inhibitory Peptides and Isolation of a Novel Peptide from Lizard Fish (Saurida elongata) Muscle Protein Hydrolysate

1
School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, China
2
Department of Pharmacy, Liuzhou Medical College, Liuzhou, Guangxi 545006, China
3
Guangxi Key Laboratory of Petrochemical Resources Processing & Process Intensification Technology, Nanning, Guangxi 530004, China
*
Author to whom correspondence should be addressed.
Mar. Drugs 2012, 10(5), 1066-1080; https://0-doi-org.brum.beds.ac.uk/10.3390/md10051066
Received: 2 March 2012 / Revised: 26 April 2012 / Accepted: 4 May 2012 / Published: 18 May 2012
Lizard fish (Saurida elongata) muscle protein was hydrolyzed using neutral protease to produce protein hydrolysate (LFPH), and the hydrolysis conditions were investigated using response-surface methodology. The optimum conditions for producing peptides with the highest angiotensin-I converting enzyme (ACE)-inhibitory activity were the following: enzyme-to-substrate ratio of 10,000 U/g, temperature of 48 °C, pH 7.0, and hydrolysis time of 2 h. Under these conditions, the ACE-inhibitory activity of LFPH and the degree of hydrolysis were 84% and 24%, respectively. A novel ACE-inhibitory peptide was isolated from LFPH using ultrafiltration, Sephadex G-15, and high-performance liquid chromatography. The amino acid sequence of the ACE-inhibitory peptide was identified as Ser-Pro-Arg-Cys-Arg (SPRCR), and its IC50 was 41 ± 1 µM. View Full-Text
Keywords: ACE-inhibitory peptide; lizard fish; enzymatic hydrolysis; response surface methodology; isolation ACE-inhibitory peptide; lizard fish; enzymatic hydrolysis; response surface methodology; isolation
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MDPI and ACS Style

Wu, S.; Sun, J.; Tong, Z.; Lan, X.; Zhao, Z.; Liao, D. Optimization of Hydrolysis Conditions for the Production of Angiotensin-I Converting Enzyme-Inhibitory Peptides and Isolation of a Novel Peptide from Lizard Fish (Saurida elongata) Muscle Protein Hydrolysate. Mar. Drugs 2012, 10, 1066-1080. https://0-doi-org.brum.beds.ac.uk/10.3390/md10051066

AMA Style

Wu S, Sun J, Tong Z, Lan X, Zhao Z, Liao D. Optimization of Hydrolysis Conditions for the Production of Angiotensin-I Converting Enzyme-Inhibitory Peptides and Isolation of a Novel Peptide from Lizard Fish (Saurida elongata) Muscle Protein Hydrolysate. Marine Drugs. 2012; 10(5):1066-1080. https://0-doi-org.brum.beds.ac.uk/10.3390/md10051066

Chicago/Turabian Style

Wu, Shanguang, Jianhua Sun, Zhangfa Tong, Xiongdiao Lan, Zhongxing Zhao, and Dankui Liao. 2012. "Optimization of Hydrolysis Conditions for the Production of Angiotensin-I Converting Enzyme-Inhibitory Peptides and Isolation of a Novel Peptide from Lizard Fish (Saurida elongata) Muscle Protein Hydrolysate" Marine Drugs 10, no. 5: 1066-1080. https://0-doi-org.brum.beds.ac.uk/10.3390/md10051066

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