Next Article in Journal
Marine Polysaccharides from Algae with Potential Biomedical Applications
Previous Article in Journal
Emerging Concepts Promising New Horizons for Marine Biodiscovery and Synthetic Biology
Article

A New Member of the TBC1D15 Family from Chiloscyllium plagiosum: Rab GTPase-Activating Protein Based on Rab7 as a Substrate

by 1,2,†, 1,2,†, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1,2, 1 and 1,2,*
1
College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China
2
Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Peer Jacobson
Mar. Drugs 2015, 13(5), 2955-2966; https://0-doi-org.brum.beds.ac.uk/10.3390/md13052955
Received: 11 January 2015 / Revised: 28 April 2015 / Accepted: 5 May 2015 / Published: 13 May 2015
APSL (active peptide from shark liver) is a hepatic stimulator cytokine from the liver of Chiloscyllium. It can effectively protect islet cells and improve complications in mice with alloxan-induced diabetes. Here, we demonstrate that the APSL sequence is present in the N-terminus of novel TBC (Tre-2, Bub2 and Cdc16) domain family, member 15 (TBC1D15) from Chiloscyllium plagiosum. This shark TBC1D15 gene, which contains an ORF of 2088 bp, was identified from a cDNA library of regenerating shark liver. Bioinformatic analysis showed that the gene is highly homologous to TBC1D15 genes from other species. Moreover, the N-terminus of shark TBC1D15 contains a motif of unknown function (DUF3548), which encompasses the APSL fragment. Rab-GAP activity analysis showed that shark TBC1D15 is a new member of the TBC1D15 family. These results demonstrated that shark TBC1D15 possesses Rab-GAP activity using Rab7 as a substrate, which is a common property of the TBC1D15 family. The involvement of APSL at the N-terminus of TBC1D15 also demonstrates that this protein might be involved in insulin signaling and may be associated with the development of type 2 diabetes. The current findings pave the way for further functional and clinical studies of these proteins from marine sources. View Full-Text
Keywords: APSL; TBC1D15 protein; Rab-GAP activity; type 2 diabetes APSL; TBC1D15 protein; Rab-GAP activity; type 2 diabetes
Show Figures

Figure 1

MDPI and ACS Style

Li, Y.; Wang, W.; Cheng, D.; Wang, T.; Lu, C.; Chen, J.; Nie, Z.; Zhang, W.; Lv, Z.; Wu, W.; Shu, J. A New Member of the TBC1D15 Family from Chiloscyllium plagiosum: Rab GTPase-Activating Protein Based on Rab7 as a Substrate. Mar. Drugs 2015, 13, 2955-2966. https://0-doi-org.brum.beds.ac.uk/10.3390/md13052955

AMA Style

Li Y, Wang W, Cheng D, Wang T, Lu C, Chen J, Nie Z, Zhang W, Lv Z, Wu W, Shu J. A New Member of the TBC1D15 Family from Chiloscyllium plagiosum: Rab GTPase-Activating Protein Based on Rab7 as a Substrate. Marine Drugs. 2015; 13(5):2955-2966. https://0-doi-org.brum.beds.ac.uk/10.3390/md13052955

Chicago/Turabian Style

Li, Yuanyuan, Weidong Wang, Dandan Cheng, Tao Wang, Conger Lu, Jian Chen, Zuoming Nie, Wenping Zhang, Zhengbing Lv, Wutong Wu, and Jianhong Shu. 2015. "A New Member of the TBC1D15 Family from Chiloscyllium plagiosum: Rab GTPase-Activating Protein Based on Rab7 as a Substrate" Marine Drugs 13, no. 5: 2955-2966. https://0-doi-org.brum.beds.ac.uk/10.3390/md13052955

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop