Discovery of Antibiofilm Activity of Elasnin against Marine Biofilms and Its Application in the Marine Antifouling Coatings
Abstract
:1. Introduction
2. Results
2.1. Isolation and Identification of Biofilm Inhibition Compounds
2.2. Elasnin Could Inhibit the Biofilm Formation of Multiple Strains of Bacteria Isolated from Marine Biofilms
2.3. Preparation of Elasnin-Based Antibiofilm Coatings
2.4. Release Rate of Elasnin from Antibiofilm Coatings
2.5. Elasnin-Based Coatings Inhibited the Formation of Multi-Species Biofilms and the Attachment of Large Biofouling Organisms in the Marine Environment
2.6. Elasnin Changed the Microbial Community Structure of Natural Marine Biofilms
2.7. Elasnin Inhibited the Larval Settlement of Balanus Amphitrite with a Low Toxic Effect
3. Discussion
4. Materials and Methods
4.1. Strains, Culture Media, and Chemicals
4.2. Bioactive Compound Isolation and Identification
4.3. Productivity Monitoring and Extraction Efficiency Comparison
4.4. MBIC Assay and MIC Assay Against Marine Bacteria
4.5. Elasnin-Based Antibiofilm Coating Preparation
4.6. Field Test and Release Rate Determination
4.7. DNA Extraction, 16S rRNA Gene Sequencing, and Analyses
4.8. Antilarval-Settlement Assay
4.9. Statistical Analyses
5. Patents
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
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Long, L.; Wang, R.; Chiang, H.Y.; Ding, W.; Li, Y.-X.; Chen, F.; Qian, P.-Y. Discovery of Antibiofilm Activity of Elasnin against Marine Biofilms and Its Application in the Marine Antifouling Coatings. Mar. Drugs 2021, 19, 19. https://0-doi-org.brum.beds.ac.uk/10.3390/md19010019
Long L, Wang R, Chiang HY, Ding W, Li Y-X, Chen F, Qian P-Y. Discovery of Antibiofilm Activity of Elasnin against Marine Biofilms and Its Application in the Marine Antifouling Coatings. Marine Drugs. 2021; 19(1):19. https://0-doi-org.brum.beds.ac.uk/10.3390/md19010019
Chicago/Turabian StyleLong, Lexin, Ruojun Wang, Ho Yin Chiang, Wei Ding, Yong-Xin Li, Feng Chen, and Pei-Yuan Qian. 2021. "Discovery of Antibiofilm Activity of Elasnin against Marine Biofilms and Its Application in the Marine Antifouling Coatings" Marine Drugs 19, no. 1: 19. https://0-doi-org.brum.beds.ac.uk/10.3390/md19010019