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Article

Characterization of Bacterial Communities and Their Antibiotic Resistance Profiles in Wastewaters Obtained from Pharmaceutical Facilities in Lagos and Ogun States, Nigeria

1
Department of Biological Sciences, Augustine University Ilara, Epe 106101, Lagos State, Nigeria
2
Department of Microbiology, University of Ibadan, Ibadan 200284, Oyo State, Nigeria
3
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
4
U.S. Salinity Laboratory, USDA-ARS, 450 West Big Springs Road, Riverside, CA 92507, USA
*
Author to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2018, 15(7), 1365; https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph15071365
Received: 31 May 2018 / Revised: 25 June 2018 / Accepted: 27 June 2018 / Published: 29 June 2018
(This article belongs to the Special Issue Microbiological Evaluation of Wastewater Treatment)
In Nigeria, pharmaceutical wastewaters are routinely disseminated in river waters; this could be associated with public health risk to humans and animals. In this study, we characterized antibiotic resistant bacteria (ARB) and their antibiotic resistance profile as well as screening for sul1 and sul2 genes in pharmaceutical wastewater effluents. Bacterial composition of the wastewater sources was isolated on non-selective media and characterized by the polymerase chain reaction (PCR) amplification of the 16S rRNA genes, with subsequent grouping using restriction fragment length polymorphism (RFLP) and sequencing. The antibiotics sensitivity profiles were investigated using the standard disk diffusion plate method and the minimum inhibitory concentrations (MICs) of selected antibiotics on the bacterial isolates. A total of 254 bacterial strains were isolated, and majority of the isolates were identified as Acinetobacter sp., Klebsiella pneumonia, Proteus mirabilis, Enterobacter sp. and Bacillus sp. A total of 218 (85.8%) of the bacterial isolates were multidrug resistant. High MICs values were observed for all antibiotics used in the study. The result showed that 31.7%, 21.7% and 43.3% of the bacterial isolates harbored sul1, sul2, and Intl1 genes, respectively. Pharmaceuticals wastewaters are potential reservoirs of ARBs which may harbor resistance genes with possible risk to public health. View Full-Text
Keywords: pharmaceutical wastewater; antibiotics-resistant bacteria (ARB); antibiotic-resistance genes (ARG), sulfonamide resistance genes; mobile genetic elements pharmaceutical wastewater; antibiotics-resistant bacteria (ARB); antibiotic-resistance genes (ARG), sulfonamide resistance genes; mobile genetic elements
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MDPI and ACS Style

Obayiuwana, A.; Ogunjobi, A.; Yang, M.; Ibekwe, M. Characterization of Bacterial Communities and Their Antibiotic Resistance Profiles in Wastewaters Obtained from Pharmaceutical Facilities in Lagos and Ogun States, Nigeria. Int. J. Environ. Res. Public Health 2018, 15, 1365. https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph15071365

AMA Style

Obayiuwana A, Ogunjobi A, Yang M, Ibekwe M. Characterization of Bacterial Communities and Their Antibiotic Resistance Profiles in Wastewaters Obtained from Pharmaceutical Facilities in Lagos and Ogun States, Nigeria. International Journal of Environmental Research and Public Health. 2018; 15(7):1365. https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph15071365

Chicago/Turabian Style

Obayiuwana, Amarachukwu, Adeniyi Ogunjobi, Min Yang, and Mark Ibekwe. 2018. "Characterization of Bacterial Communities and Their Antibiotic Resistance Profiles in Wastewaters Obtained from Pharmaceutical Facilities in Lagos and Ogun States, Nigeria" International Journal of Environmental Research and Public Health 15, no. 7: 1365. https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph15071365

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