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Article

Development and Validation of Green UV Derivative Spectrophotometric Methods for Simultaneous Determination Metformin and Remogliflozin from Formulation: Evaluation of Greenness

1
Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al Hofuf 31982, Saudi Arabia
2
Department of Pharmaceutics, Vidya Siri College of Pharmacy, Bangalore 560035, India
3
Department of Biotechnology and Food Technology, Durban University of Technology, Durban 4001, South Africa
4
Department of Biomedical Sciences, College of Clinical Pharmacy, King Faisal University, Al Hofuf 31982, Saudi Arabia
*
Author to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2021, 18(2), 448; https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph18020448
Received: 19 November 2020 / Revised: 3 January 2021 / Accepted: 5 January 2021 / Published: 8 January 2021
(This article belongs to the Collection Environmental Chemistry and Technology)
The recent trend in green analytical chemistry is the development of green analytical methods using environmentally friendly solvents. Therefore, three ecofriendly manipulated UV spectroscopic techniques have been validated for the concurrent quantification of newly approved remogliflozin etabonate (REM) and metformin HCl (MET) tablets using water as a solvent. The first method was established using first derivative absorption spectroscopic method by determining the peak amplitude at 233.0 nm for REM and 252.2 nm for MET, a zero crossing of one the component. The second and third methods were based on the peak amplitude difference and first-order derivative absorption of the ratio spectra developed by the manipulation of scanned UV spectra. REM and MET showed good linearity in the series of 1–20 µg ml−1 and 2.5–35 µg ml−1, respectively, by all three methods with an excellent correlation coefficient (r2 ≥ 0.998). Further, the proposed UV spectroscopic techniques were validated as per International Council for Harmonization guidelines. The methods showed good sensitivity, accuracy, and precision. Anticipated procedures were effectively utilized for the concurrent quantification of REM and MET in laboratory prepared mixtures and tablets. The high percent recovery with low standard deviation found for both analytes by all three methods confirms the accuracy and precision of the procedures. Finally, the greenness of the proposed spectroscopic methods, evaluated by semi-quantitative and quantitative methods, showed the eco-friendly nature of the methods. Furthermore, the proposed approaches were simple, accurate, sensitive, economic, and environmentally friendly and hence can be utilized for regular quality control of REM and MET formulation. View Full-Text
Keywords: remogliflozin; metformin; UV derivative spectroscopy; validation; formulation; ecofriendly remogliflozin; metformin; UV derivative spectroscopy; validation; formulation; ecofriendly
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MDPI and ACS Style

Attimarad, M.; Nair, A.B.; Sreeharsha, N.; Al-Dhubiab, B.E.; Venugopala, K.N.; Shinu, P. Development and Validation of Green UV Derivative Spectrophotometric Methods for Simultaneous Determination Metformin and Remogliflozin from Formulation: Evaluation of Greenness. Int. J. Environ. Res. Public Health 2021, 18, 448. https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph18020448

AMA Style

Attimarad M, Nair AB, Sreeharsha N, Al-Dhubiab BE, Venugopala KN, Shinu P. Development and Validation of Green UV Derivative Spectrophotometric Methods for Simultaneous Determination Metformin and Remogliflozin from Formulation: Evaluation of Greenness. International Journal of Environmental Research and Public Health. 2021; 18(2):448. https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph18020448

Chicago/Turabian Style

Attimarad, Mahesh, Anroop B. Nair, Nagaraja Sreeharsha, Bandar E. Al-Dhubiab, Katharigatta N. Venugopala, and Pottathil Shinu. 2021. "Development and Validation of Green UV Derivative Spectrophotometric Methods for Simultaneous Determination Metformin and Remogliflozin from Formulation: Evaluation of Greenness" International Journal of Environmental Research and Public Health 18, no. 2: 448. https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph18020448

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