Next Article in Journal
Factors Associated with Smoke-Free Pregnancy among Aboriginal and Torres Strait Women and Their Experience of Quitting Smoking in Pregnancy: A Mixed Method Cross-Sectional Study
Next Article in Special Issue
Acute Effects of Ischemic Intra-Conditioning on 30 m Sprint Performance
Previous Article in Journal
Individual and Contextual Factors Associated with Malaria among Children 6–59 Months in Nigeria: A Multilevel Mixed Effect Logistic Model Approach
Previous Article in Special Issue
Effects of a Low-Carbohydrate High-Fat Diet Combined with High-Intensity Interval Training on Body Composition and Maximal Oxygen Uptake: A Systematic Review and Meta-Analysis
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Review

Effects of Resistance Training Performed with Different Loads in Untrained and Trained Male Adult Individuals on Maximal Strength and Muscle Hypertrophy: A Systematic Review

1
Postgraduate Program in Physical Education, Federal University of Juiz de Fora (UFJF), Juiz de Fora 36036-900, Brazil
2
Strength Training Research Laboratory, Federal University of Juiz de Fora (UFJF), Juiz de Fora 36036-900, Brazil
3
Laboratory of Exercise Physiology and Morphofunctional Evaluation (LABFEX), Granbery Methodist College, Juiz de Fora 36010-359, Brazil
4
Department of Medical Sciences, The Wojciech Korfanty School of Economics, 40-065 Katowice, Poland
5
Study Group and Research in Neuromuscular Responses, Federal University of Lavras (UFLA), Lavras 37200-900, Brazil
6
Postgraduate Program in Physical Education, Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro 21941-901, Brazil
7
Institute of Sport Sciences, Jerzy Kukuczka Academy of Physical Education in Katowice, 40-065 Katowice, Poland
*
Author to whom correspondence should be addressed.
Int. J. Environ. Res. Public Health 2021, 18(21), 11237; https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph182111237
Submission received: 27 September 2021 / Revised: 20 October 2021 / Accepted: 22 October 2021 / Published: 26 October 2021

Abstract

:
The load in resistance training is considered to be a critical variable for neuromuscular adaptations. Therefore, it is important to assess the effects of applying different loads on the development of maximal strength and muscular hypertrophy. The aim of this study was to systematically review the literature and compare the effects of resistance training that was performed with low loads versus moderate and high loads in untrained and trained healthy adult males on the development of maximal strength and muscle hypertrophy during randomized experimental designs. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines (2021) were followed with the eligibility criteria defined according to participants, interventions, comparators, outcomes, and study design (PICOS): (P) healthy males between 18 and 40 years old, (I) interventions performed with low loads, (C) interventions performed with moderate or high loads, (O) development of maximal strength and muscle hypertrophy, and (S) randomized experimental studies with between- or within-subject parallel designs. The literature search strategy was performed in three electronic databases (Embase, PubMed, and Web of Science) on 22 August 2021. Results: Twenty-three studies with a total of 563 participants (80.6% untrained and 19.4% trained) were selected. The studies included both relative and absolute loads. All studies were classified as being moderate-to-high methodological quality, although only two studies had a score higher than six points. The main findings indicated that the load magnitude that was used during resistance training influenced the dynamic strength and isometric strength gains. In general, comparisons between the groups (i.e., low, moderate, and high loads) showed higher gains in 1RM and maximal voluntary isometric contraction when moderate and high loads were used. In contrast, regarding muscle hypertrophy, most studies showed that when resistance training was performed to muscle failure, the load used had less influence on muscle hypertrophy. The current literature shows that gains in maximal strength are more pronounced with high and moderate loads compared to low loads in healthy adult male populations. However, for muscle hypertrophy, studies indicate that a wide spectrum of loads (i.e., 30 to 90% 1RM) may be used for healthy adult male populations.

1. Introduction

Resistance training is an exercise intervention that is used to develop maximal strength and muscle hypertrophy [1]. Commonly, this type of exercise is used to improve athletic performance and functional capacity during the activities of daily living in healthy and clinical populations [2,3,4]. In this way, proper manipulation of variables that make up resistance training, such as muscle action, external load, number of performed repetitions, sets, rest intervals, movement tempo, type and sequence of exercises, frequency of training, and level of effort, are considered fundamental components for maximizing neural and morphological adaptations [5,6]. Moreover, the magnitude of the load, referred to as the amount of external load during one or more sets of a given exercise, is characterized as one of the most significant aspects of resistance training programs [5,7]. The American College of Sports Medicine guidelines have declared that loads ≥80% of 1 repetition maximum (RM) are required to enhance maximal strength, while loads between 70 and 85% 1RM are necessary to increase muscle hypertrophy [5]. These recommendations are based on the repetition continuum, which proposes that the number of repetitions performed at a given load will result in specific adaptations [8].
The repetition continuum is supported by some theories, one of them is the size principle proposed by Henneman et al. [9], which advocates that higher training loads must be used to recruit motor units with a higher excitability threshold and, thus, achieve optimal muscle adaptations. On the other hand, Delorme [10] proposed that high loads increase maximal strength/power while low loads improve muscular endurance. Previous studies have provided partial support for Delorme’s hypothesis, underpinning what is currently accepted as theory [11,12]. Although some guidelines suggest the use of high and moderate loads to development maximal strength and muscle hypertrophy, several studies showed increases in maximal strength and muscle hypertrophy after resistance training with low loads (i.e., <60% 1RM) [13,14,15,16,17]. These studies are in line with recent guidelines indicating that the athletic population may achieve comparable muscle hypertrophy across a wide spectrum of loading zones [18]. However, meta-analysis data provided no differences in muscle hypertrophy between high and low loads (≥80% 1RM, or ≤8RM vs. <60% 1RM, or >15RM), moderate and low loads (60–79% 1RM, or 9–15RM vs. <60% 1RM, or >15RM), or high and moderate loads (≥80% 1RM, or ≤8RM vs. 60–79% 1RM, or 9–15RM) during resistance training performed until volitional failure in healthy adults [19]. Muscle failure was shown to be an important factor for low loads to be as effective as high loads regarding muscle hypertrophy [20,21,22,23]. On the other hand, meta-analytic data confirmed that for optimal strength gains, high loads are necessary. For example, Schoenfeld et al. [24] reported a moderate-to-large effect size (ES) difference (ES = 0.58) favoring high- (>60% 1RM) vs. low- (60% 1RM) load training based on pooled data from 14 included studies.
Recently, the role of loading during resistance training aiming at skeletal muscle hypertrophy has been widely discussed [25]. Schoenfeld et al. [26] assessed the changes in maximal strength and muscle hypertrophy of the soleus (predominantly slow twitch fibers) and gastrocnemius muscles (similar composition of slow and fast twitch fibers) using low (i.e., 20–30RM) and high loads (i.e., 6–10RM) and found no additional benefit to muscle hypertrophy when resistance training was based on muscle fiber composition. The results indicated that muscle hypertrophy seems to be independent of muscle fiber type and the load used during the resistance training program, as long as the sets are performed with a high level of effort. Furthermore, a recent meta-analysis found no significant differences between low- and high-load resistance training on type I or type II muscle fiber hypertrophy [27]. Other studies also reported that 10 weeks of resistance training to volitional failure in untrained males at low and high loads (30% and 80% 1RM) resulted in similar increases in muscle fiber cross-sectional area (CSA) [23,28]. In contrast, when comparing low repetitions (3–5RM), intermediate repetitions (9–11RM), and high repetitions (20–28RM), Campos et al. [29] concluded that the three main types of muscle fibers (types I, IIA, and IIX) were increased in the low- and intermediate-repetitions groups; however, maximal strength development was greater for the low-repetitions group.
Understanding how the load can interfere on the development of maximal strength and muscle hypertrophy is critical for coaches and resistance training practitioners to be able to design more efficient training programs. However, previous systematic reviews and meta-analyses examined the development of maximal strength and muscle hypertrophy over very wide load ranges (i.e., low (≤60% 1RM) and high loads (>60% 1RM or ≥65% 1RM)) [24,30]. This division of load ranges can be a mistake for not considering the previous guidelines that divided resistance training into low (<67% 1RM or >12 repetitions), moderate (between 67 and 85% 1RM or 6–12 repetitions), and high loads (>85% 1RM or <6 repetitions) [7]. In addition, this division into a wide load range may also make it difficult to analyze the hypertrophic response and maximal strength at a well-defined load spectrum, which may extrapolate the effectiveness of the load ranges that were established and recommended in these studies and compromise the resistance training prescription. Thus, the aim of the present study was to systematically review the literature and compare the effects of resistance training performed with low loads versus moderate and high loads in untrained healthy adult males on the development of maximal strength and muscle hypertrophy during randomized experimental designs.

2. Materials and Methods

This systematic review followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines [31,32].

2.1. Eligibility Criteria

Following the PRISMA guidelines, relevant studies were included according to participants, intervention, comparators, outcomes, and study design (PICOS) [33] inclusion criteria (Table 1). It is noteworthy that only studies in the English language and published in peer-reviewed journals were included. Furthermore, training status was established according to untrained: individuals who had not been consistently trained for 1 year; recreationally trained: individuals training consistently for 1–5 years; highly trained: individuals training for at least 5 years [34].

2.2. Information Sources

Searches were performed in three electronic databases (Embase, PubMed, and Web of Science) on 22 August 2021. The studies were retrieved from an electronic database search, comprehensive sweeping in the reference list of the included studies, and systematic reviews with meta-analyses previously published on load and chronic adaptations, more specifically on the development of maximal strength and muscle hypertrophy.

2.3. Search Strategy

The search strategy combined the descriptors using the Booleans operators (AND/OR/NOT) in the following way: (“resistance training” OR “strength training” OR “resistance exercise”) AND (“high-load” OR “high load” OR “high-intensity” OR “high intensity” OR “heavy loads” OR “low-load” OR “low load” OR “low-intensity” OR “low intensity” OR “volume training” OR “training load”) AND (“hypertrophy” OR “muscle size” OR “skeletal muscle enlargement” OR “muscle thickness” OR “muscle mass” OR “muscle fibers, skeletal” OR “muscle, skeletal” OR “growth” OR “cross-sectional area” OR “muscle strength” OR “dynamic strength” OR “dynamic force” OR “maximum repetition” OR “1RM” OR “isometric contraction” OR “isometric force” OR “maximal voluntary contraction” OR “MVC” OR “maximal voluntary isometric contraction” OR “MVIC”) (Supplementary Materials).

2.4. Selection Process

The studies that were retrieved from each database were clustered using the EndNote X9 software (Clarivate Analytics, Philadelphia, PA, USA) and duplicate studies were automatically and manually removed. The titles and abstracts were assessed according to the eligibility criteria by two independent researchers (M.L. and D.S.). Conflicts were settled by a third reviewer (J.G.V.). The researchers were not blinded to authors, institutions, or journals. The abstracts not offering enough information to be evaluated were sent to the next phase, in which the full text was read.

2.5. Data Collection Process and Data Items

Two independent reviewers (M.L. and D.S.) extracted the data from the full texts. Data were recorded in Excel spreadsheets that were created specifically for this review. The data that was collected covered the characteristics of participants (sample size, age, height, body mass, and training status) and characteristics of the studies (study design, time of analysis, resistance exercise(s), prescription, weekly frequency, movement tempo, volume, and findings). After the selection, the data extracted by both reviewers were compared and the divergences were decided by all three reviewers (M.L., D.S., and J.G.V.).

2.6. Methodological Quality Assessment

After the literature search and selection, a methodological quality assessment was performed independently by two authors (J.E.F. and J.G.V.) using the Physiotherapy Evidence Database (PEDro) scale, which is a valid measure of the methodologic quality of randomized trials [35] and displays acceptable inter-rater reliability [35,36]. Thus, scores on the PEDro scale ranged from 0 (low methodological quality) to 10 (high methodological quality). The quality of the studies was used for qualitative assessment, and it was not used as an exclusion criteria. The methodological quality of the study was categorized as follows: a score ranging from 6 to 10 was indicative of high quality, whereas scores of 4–5 indicated moderate quality and scores ≤3 indicated low quality [37].

2.7. Synthesis Methods

A quantitative analysis of the data (meta-analysis) was not performed. A narrative synthesis of the results was provided.

3. Results

3.1. Study Selection

A flow diagram of the literature search is presented in Figure 1. A total of 3913 studies were generated by the database search. Afterward, 1778 duplicate studies were removed. In addition, another 2091 studies were removed after analyzing each title and abstract. Then, 54 studies were considered for the systematic review, with 10 studies considered by checking the reference list of included studies. After analyzing the eligibility criteria, 31 studies were excluded and, finally, 23 studies remained for the qualitative synthesis.

3.2. Study Characteristics

Table 2 shows the characteristics of the participants in the 23 studies that were selected for systematic review regarding the sample size, age, height, weight, and training status (mean ± SD) of the 563 participants, where 454 were untrained (80.6%) [11,14,15,16,17,21,22,23,26,28,29,38,39,40,41,42,43,44,45] and 109 were recreationally trained (19.4%) [13,46,47] in resistance training.
Table 3 shows the characteristics of the studies that were selected for the systematic review regarding the study design, time of analysis, resistance exercise(s), prescription, weekly frequency, movement tempo, volume, and findings. Regarding the assessment of maximal strength development, 13 studies assessed dynamic strength using 1RM (56.5%) [11,13,14,15,17,21,22,23,29,44,46,47,48] and another four studies assessed the isometric strength (17.4%) [26,38,39,45]. In addition, five studies simultaneously assessed dynamic strength using 1RM and isometric strength using a maximal voluntary isometric contraction (MVIC) (21.7%) [16,28,41,42,43], and, finally, one study assessed isometric strength using maximal isometric voluntary torque (4.4%) [40]. Eight studies assessed muscle hypertrophy using ultrasound (34.8%) [14,15,17,22,26,42,43,47], whereas four verified it using a biopsy (17.4%) and six using magnetic resonance imaging (26.1%) [16,21,38,39,41,45]. In addition, one study assessed muscle hypertrophy using a biopsy and magnetic resonance imaging (4.4%) [28]. Another four studies did not perform any muscle hypertrophy assessment (17.4%) [11,13,40,48].
Regarding maximal strength development measured by the 1RM test, 18 studies indicated a significant improvement between pre- and post-intervention using low-, moderate-, and high-load training protocols [11,13,14,15,16,17,21,22,23,26,28,29,41,43,44,46,47,48]. However, when a t-test for independent samples or ANOVA were used to compare differences between groups, most studies found that the moderate- and high-load groups significantly improved their 1RM compared to the low-load group [11,17,22,28,29,41,42,43,46,48], although eight studies did not observe differences between these groups [13,14,15,16,21,23,26,44]. Greater increases in isometric strength using the MVIC test were also observed in the moderate- and high-load groups compared to the low-load groups [16,38,39,41,42,43].
For muscle hypertrophy, 16 studies showed that the low-, moderate-, and high-load groups improved the cross-sectional area and muscle thickness between pre- and post-intervention [14,15,17,21,22,23,26,28,38,39,41,42,43,45,46,47]. Moreover, comparisons between groups (i.e., low, moderate, and high load) did not show significant differences in the cross-sectional area or muscle thickness [14,15,17,21,23,26,28,38,39,42,43,45,46,47] when the repetitions of each set were performed to muscular failure [21]. However, low loads (i.e., ≤20%) seemed to be ineffective for muscle growth [22].
Table 3. Summary and characteristics of the studies included in the review.
Table 3. Summary and characteristics of the studies included in the review.
StudyDesignDuration (Weeks)ExercisePrescriptionFrequency (Days)Movement TempoVolumeOutcomes MeasuresFindings
Anderson and Kearney [11]Between-subject9Bench pressLL 1: 1 set 100–150RM
LL 2: 2 sets 30–40RM
ML: 3 sets 6–8RM
2 min interval
3LL 1 and LL 2: 40 reps/minNot equalized1RM strength (bench press)1RM: ↑ pre- to post-intervention in all groups (ML > LL 1 and LL 2)
Campos et al. [29]Between-subject8Leg press, squat, and leg extensionLL: 2 sets 20–28RM, 1 min interval
ML: 3 sets 9–11RM, 2 min interval
HL: 4 sets 3–5RM, 3 min interval
2 (4 weeks)
3 (4 weeks)
Not reportedEqualized1RM strength (squat, leg press, and knee extension)
Muscle fCSA (biopsy of VL)
1RM: ↑ pre- to post-intervention in all groups (leg press and back squat—HL > ML and LL; leg extension—HL > LL)
CSA: ↑ pre- to post-intervention in ML and HL
Fink et al. [38]Between-subject8Barbell curl, preacher curl, hammer curl, close grip bench press, french press, and dumbbell extensionLL: 3 sets 20RM, 30 s interval
ML: 3 sets 8RM, 3 min interval
31 s concentric
2 s eccentric
Not equalizedMVIC strength (elbow flexors)
Muscle CSA (MRI of elbow flexors)
MVIC: ↑ pre- to post-intervention in ML
CSA: ↑ pre- to post-intervention in all groups
Fink et al. [39]Between-subject8Unilateral bicep preacher curlLL: 3 sets 30% 1RM to volitional failure
ML: 3 sets 80% 1RM to volitional failure
31 s concentric
2 s eccentric
Not equalizedMVIC strength (elbow flexors)
Muscle CSA (MRI of elbow flexors)
MVIC: ↑ pre- to post-intervention in ML
CSA: ↑ pre- to post-intervention in all groups
Fisher et al. [40]Within-subject6Unilateral leg extensionLL: 3 sets 50% MVIT to failure,
ML: 3 sets 80% MVIT to failure,
2 min interval
12 s concentric
1 s isometric
3 s eccentric
Not equalizedMVIT strength (knee extension)MVIT: ↑ pre- to post-intervention in all conditions
Holm et al. [41]Within-subject12Unilateral leg extensionLL: 10 sets 36 (15.5% 1RM)
ML: 10 sets 8 (70% 1RM)
3LL: 5 s per repetition
ML: 3 s per repetition
Equalized1RM strength (knee extension)
MVIC strength (knee extension)
Muscle CSA (MRI of QF)
1RM: ↑ pre- to post-intervention in all groups (ML > LL)
MVIC: ↑ pre- to post-intervention in ML
CSA: ↑ pre- to post-intervention in all groups (ML > LL)
Jenkins et al. [42]Between-subject4Dumbbell bicep curlsLL: 3 sets 30% 1RM to failure
ML: 3 sets 80% 1RM to failure
2 min interval
31 s concentric
1 s eccentric
Equalized1RM strength (elbow flexion)
MVIC strength (elbow flexion)
Muscle thickness (ultrasound of BB and BR)
1RM: ↑ pre-intervention to post 2 and 4 weeks in ML
MVIC: ↑ pre-intervention to post 2 and 4 weeks in ML
MT: ↑ pre-intervention to post 2 and 4 weeks in all groups
Jenkins et al. [43]Between-subject6Leg extensionLL: 3 sets 30% 1RM to failure
ML: 3 sets 80% 1RM to failure
2 min interval
31 s concentric
1 s eccentric
Equalized1RM strength (knee extension)
MVIC strength (knee extension)
Muscle thickness (ultrasound of VL, VM, and RF)
1RM: ↑ pre- to post-intervention in all groups (ML > LL)
MVIC: ↑ pre- to post-intervention in all groups (ML > LL)
MT: ↑ pre- to post-intervention in all groups
Jones et al. [48]Between-subject10Squat (olympic style), Romanian dead lift, lunge, half back squatLL: 4 sets 5–15 (40–60% 1RM)
ML: 4 sets 3–10 (70–90% 1RM)
Both groups performed 3 sets with full range of motion and one set with partial range of motion
2Not reportedNot equalized1RM strength (squat)1RM: ↑ pre- to post-intervention in all groups (ML > LL)
Lasevicius et al. [22]Within-subject12Unilateral leg press and unilateral bicep curlLL (20%): 3 sets at 20% 1RM to concentric muscle failure
LL (40%): 4 sets at 40% 1RM to volitional failure
LL: 5 sets at 60% 1RM to volitional failure
ML: 4 sets at 80% 1RM to volitional failure
2 min interval
22 s concentric
2 s eccentric
Equalized1RM strength (leg press and elbow flexion)
Muscle thickness (ultrasound of elbow flexors and VL)
1RM bicep curl: ↑ pre- to post-intervention in all conditions (80% > 20, 40, and 60%) 1RM leg press: ↑ pre- to post-intervention in all conditions (60 and 80% > 20 and 40%)
CSA: ↑ pre- to post-intervention in all conditions (80, 60, and 40% > 20%)
Lasevicius et al. [21]Within-subject8Unilateral leg extensionLL to failure: 3 sets at 30% 1RM
LL not to failure: 3 sets at 30% 1RM
ML to failure: 3 sets at 80% 1RM
ML not to failure: 3 sets at 80% 1RM
2 min interval
2Not reportedEqualized1RM strength (knee extension)
Muscle CSA (MRI of QF)
1RM: ↑ pre- to post-intervention in all conditions (ML failure = ML not to failure > LL failure = LL not to failure)
CSA: ↑ pre- to post-intervention in ML failure, ML not to failure, and LL failure
Lim et al. [23]Between-subject10Leg press, leg extension, and leg curlLL: 3 sets 30% 1RM to volitional failure
LL WM: 3 sets 30% 1RM (work matched to HL)
ML: 3 sets 80% 1RM to volitional failure
Interval not reported
3Not reportedEqualized to LL WM and ML1RM strength (leg extension)
Muscle fCSA (biopsy of VL)
1RM: ↑ pre- to post-intervention in all groups
CSA: ↑ pre- to post-intervention in type I in LL and ML; ↑ pre- to post-intervention in type II in all groups
Lopes et al. [13]Between-subject6Session A: bench press (flat and inclined), fly, bicep curls (standing and concentrated), squat, leg press, and abdominal;
Session B: back lat pull-down, one arm dumbbell row, row (open grip), tricep overhead extension, tricep pushdown, leg curl, calf raise, and abdominal
LL: 3 sets 20RM
ML: 6 sets 10RM
1 min interval
4Not reportedEqualized1RM strength (bench press and squat)1RM: ↑ pre- to post-intervention in bench press and back squat in all groups
Mitchell et al. [28]Within-subject10Unilateral leg extensionLL: 3 sets 30% 1RM to point of fatigue
ML: 1 set 80% 1RM to voluntary failure
ML: 3 sets 80% 1RM to point of fatigue
1 min interval
3Not reportedNot equalized1RM strength (knee extension)
MVIC strength (knee extensors)
Muscle CSA (MRI of QF)
Muscle fCSA (biopsy of VL)
1RM: ↑ pre- to post-intervention in all conditions (ML 1 and ML 3 > LL)
CSA: ↑ pre- to post-intervention in all groups
Morton et al. [46]Between-subject12Monday/Thursday: leg press/seated row, barbell bench press/cable hamstring curl, and front planks; Tuesday/Friday: machine-guided shoulder press/bicep curls, tricep extension/wide-grip pulldown, and machine-guided leg extensionLL: 3 sets 20–25 (30–50% 1RM) volitional failure
ML: 3 sets 8–12 (75–90% 1RM) volitional failure
1 min interval
4Not reportedNot equalized1RM strength (bench press, leg press, shoulder press, and knee extension)
Muscle fCSA (biopsy of VL)
1RM: ↑ pre- to post-intervention in all groups (ML > LL in bench press)
CSA: ↑ pre- to post-intervention in all groups
Netreba et al. [44]Between-subject8Leg pressLL: 7 sets 35–50 (20–25% 1RM), 5–6 min interval
ML: 5 sets (2 × 40–50 s (60–70% 1RM), 5–6 min interval
HL: 6–10 reps (85–90% 1RM), 5–6 min interval
3LL: high velocity
ML: low velocity
HL: average velocity
Equalized1RM strength (knee extension)
Muscle fCSA (biopsy of VL)
1RM: ↑ pre- to post-intervention in LL and HL in all velocities; ↑ pre- to post-intervention in ML for 180°/s, 300°/s, and isometric strength
CSA: ↑ pre- to post-intervention in type I and II in all groups, except type II of the LL groups
Nóbrega et al. [14]Within-subject12Unilateral leg extensionLL to failure and LL to volitional interruption: 3 sets 30% 1RM
ML to failure and ML to volitional interruption: 3 sets 80% 1RM
2 min interval
2Not reportedNot equalized1RM strength (knee extension)
Muscle CSA (ultrasound of VL)
1RM: ↑ pre- to post-intervention in all groups
CSA: ↑ pre- to post-intervention in all groups
Popov et al. [45]Between-subject8Leg pressLL: session 1-3 sets (4 × 50–60 s 50% MVIC) + session 2 and 3-1 set (4 × 50–60 s), 10 min interval
ML: session 1-7 sets 6–12 (80% MVIC) 10 min interval + session 2 and 3-3 sets 6-12 (80% MVIC), 10 min interval
3Not reportedNot equalizedMVIC strength (knee extensors)
Muscle CSA (MRI of QF and GM)
MVIC: ↑ pre- to post-intervention in all groups
CSA: ↑ pre- to post-intervention in all groups
Schoenfeld et al. [47]Between-subject8Flat barbell press, barbell military press, wide-grip lat pulldown, seated cable row, squat, leg press, and leg extensionLL: 3 sets 25–35 (30–50% 1RM) to failure
ML: 3 sets 8–12 (70–80% 1RM) to failure
90 s interval
3 days per week1 s concentric
2 s eccentric
Not equalized1RM strength (squat and bench press)
Muscle thickness (ultrasound of elbow flexors, elbow extensors, and QF)
1RM: ↑ pre- to post-intervention in all groups, except in bench press in LL
CSA: ↑ pre- to post-intervention in all groups
Schoenfeld et al. [26]Within-subject8Seated plantar flexion and standing plantar flexionLL: 4 sets 20–30RM
ML: 4 sets 6–10RM
90 s interval
2Controlled concentric
~2 s eccentric
Not equalizedMVIC strength (plantar flexors)
Muscle thickness (ultrasound of medial gastrocnemius, lateral gastrocnemius, and soleus)
MVIC: ↑ pre- to post-intervention in all groups
MT: ↑ pre- to post-intervention in all groups
Tanimoto and Ishii [16]Between-subject12Leg extensionLL 1: 3 sets 50% 1RM to failure
LL 2: 3 sets 50% 1RM to failure
ML: 3 sets 80% 1RM to failure
1 min interval
3 days per weekLL 1: 3 s eccentric and 3 s concentric with 1 s pause with no relaxation
LL 2 and ML: 1 s eccentric and 1 s concentric with 1 s pause
Not equalized1RM strength (knee extension)
MVIC strength (knee extensors)
Muscle CSA (MRI of QF)
1RM: ↑ pre- to post-intervention in all groups
MVIC: ↑ pre- to post-intervention in ML
CSA: ↑ pre- to post-intervention in ML
Tanimoto et al. [17]Between-subject13Squat, bench press, lat pulldown, abdominal bend, and back extensionLL: 3 sets ~ 55–60% 1RM to failure
ML: 3 sets ~ 80–90% 1RM to failure
1 min interval
2LL: 3 s concentric
3 s eccentric
HL: 1 s concentric
1 s eccentric
1 s rest
Not equalized1RM strength (squat, chest press, lat pull- down, ab bend, back extension, and knee extension)
Muscle thickness (ultrasound of chest, anterior upper arm, posterior upper arm, abdomen, subscapula, anterior thigh, and posterior thigh)
1RM: ↑ pre- to post-intervention in all groups (back extension ML > LL)
MT: ↑ pre- to post-intervention in all groups
Tanimoto et al. [15]Between-subject13SquatLL: 3 sets ~ 55–60% 1RM to failure
ML: 3 sets ~ 85–90% 1RM to failure
1 min interval
2LL: 3 s concentric
3s eccentric
HL: 1 s concentric
1 s eccentric
1 s rest
Not equalized1RM strength (squat)
Muscle thickness (ultrasound of anterior thigh and posterior thigh)
1RM: ↑ pre- to post-intervention in all groups
MT: ↑ pre- to post-intervention in all groups
BB: biceps brachii; BR: brachialis; CSA: cross-sectional area; fCSA: fiber cross-sectional area; GM: gluteus maximus; HL: high load; LL: low load; min: minutes; ML: moderate load; MRI: magnetic resonance imaging; MT: muscle thickness; MVIC: maximum isometric voluntary contraction; MVIT: maximal voluntary isometric torque; QF: quadriceps femoris; RF: rectus femoris; RM: repetition maximum; s: seconds; VL: vastus lateralis; VM: vastus medialis; WM: work matched; ↑: denotes significant increases; ↓: denotes significant decreases; >: denotes significant difference between groups; ~: approximately.

3.3. Methodological Quality in the Included Studies

Table 4 shows the average quality rating of studies that were evaluated using the PEDro scale. The average of 5.7 ± 0.9 indicated that the set of studies that were selected for this systematic review had moderate quality; furthermore, none of them were considered low quality.

4. Discussion

This study aimed to systematically review the literature and compare the effects of resistance training that was performed with low loads versus moderate and high loads in untrained healthy adult males on the development of maximal strength and muscle hypertrophy during randomized experimental designs. The main findings indicated that the load magnitude used during resistance training influences the development of maximal dynamic and isometric strength. In general, comparisons between groups (i.e., low, moderate, and high loads) showed higher gains in 1RM and MVIC when moderate and high loads were used. On the other hand, regarding muscle hypertrophy, most studies showed that when resistance training was undertaken to muscle failure, the load used seemed to have a lesser influence on muscle hypertrophy. We emphasize that all studies were classified as being of moderate to high methodological quality, although only two studies scored higher than six points.

4.1. Effects of Different Loads on Maximal Strength Development

Several studies suggested that performing resistance training with a high load is necessary to maximize gains in 1RM strength [11,17,21,22,28,29,42,43,46,47], although low loads were also shown to be effective in increasing these gains [11,13,14,15,16,17,22,23,44,46,47]. In this regard, a recent systematic review with a meta-analysis proposed by Schoenfeld et al. [24] showed that resistance training performed with high and low loads translated into percentage gains with 1RM of 35.4 and 28.0%, respectively. In contrast, Lasevicius et al. [22] observed a plateau in maximal strength development after 6 weeks of resistance training with low loads. Moreover, after 12 weeks, the groups that performed resistance training with high loads showed significant improvements in maximal strength compared to low load groups. As most studies assessed untrained individuals [11,14,15,16,17,21,22,28,29,42,43,44], it is possible to suggest that the early phases of resistance training were primarily impacted by enhancements in motor learning and coordination [49]. Therefore, low load training schemes might provide a sufficient stimulus to increase maximal strength. Untrained individuals generally have a lower coordination level for performing resistance exercises [22]. Thus, it seems that resistance training with low loads may be adequate to generate neural adaptations [50] and allow muscles to be controlled more effectively within the context of the task [51], thereby increasing maximal strength. However, it should be noted that the ability to produce force is a result of a combination of neural factors [52], muscle mass [53], and the specificity of the load during resistance training (i.e., high loads) [22]. Consequently, it is undeniable that heavier load training may be increasingly important to achieve maximal strength gains as the individual becomes more experienced in resistance training [24,30]. In this line, high loads may be more effective at increasing the recruitment of motor units and promote changes in agonist-antagonist co-activation ratios in the long term compared to low loads [54].
Other studies also showed that heavier loads were superior to lighter loads at improving MVIC [38,39,42,43]. In this way, Schoenfeld et al. [26] found no difference between low and moderate loads on MVIC. Thus, it seems that high loads may lead to greater increases in neural drive compared to low loads [54,55], improving performance in this specific situation. Despite this, few studies assessed the effect of resistance training that was performed to muscle failure and not performed to muscle failure, with the objective of maximal strength development [21,22]. There is a consensus that muscle failure is not necessary to maximize strength development in untrained males [14,21]. Thus, it is possible to infer that muscle failure is not a key factor for increasing maximal strength, although studies with trained male individuals are still needed to confirm this hypothesis.

4.2. Effects of Different Loads on Muscle Hypertrophy Development

Most of the studies included in this review showed no significant difference in muscle hypertrophy and muscle thickness when low, moderate, and high loads were compared [14,15,17,21,22,23,28,29,38,42,43,44,45,46,47]. Although these results are in agreement with the systematic reviews and meta-analyses that were previously published [19,24,30], we highlight that few experimental studies used the biopsy technique for a direct histological assessment of muscle hypertrophy [23,28,29,44,46]. In this way, conclusions based on the current literature can only be extrapolated to imaging techniques that have assessed the muscle as a whole (i.e., ultrasound and magnetic resonance imaging). Indeed, there is contradictory evidence regarding the agreement between imaging and histological techniques regarding muscle hypertrophy determination [56] in which it is not possible to state whether type I and II fibers respond similarly to resistance training schemes (i.e., low and high loads) [57]. In untrained male individuals, Campos et al. [29] observed that after 6 weeks of resistance training for lower limbs, the low-load group did not show a significant increase in type I fibers, while the high-load group demonstrated a significant increase in type I, IIa, and mainly IIX fibers. In contrast, Mitchell et al. [28] and Lim et al. [23] verified a significant increase for all types of muscle fibers for both resistance training schemes (i.e., low and high load). To assess trained male individuals, Morton et al. [46] randomized 49 young males with an average experience of 4 years in resistance training for 12 weeks of training sessions for the whole body using low- and high-load schemes. The results of this study showed that both load schemes yielded similar rates of muscle hypertrophy in all types of fibers that were assessed. In isolation, these results suggest that individuals may present similar growth in all types of muscle fibers regardless of the load scheme used. However, it is currently impossible to determine whether the hypertrophic potential of each type of fiber for a given load scheme may increase or decrease over time [57] since most studies that investigated this topic were short in duration [23,28,46]. It is important to highlight that the muscle biopsy technique also had significant limitations, such as the extraction of a small portion of the skeletal muscle that may not necessarily reflect the muscle hypertrophy of the analyzed muscle as a whole [57]. Additionally, studies that applied this technique chose the vastus lateralis for the analysis [23,28,29,46], which limits the extrapolation of results to other muscles that might respond differently to the same exercise protocol. Another important point that is currently discussed is whether muscle hypertrophy may be enhanced by applying lighter loads on muscles that are predominantly composed of type I fibers and heavier loads on muscles that are predominantly composed of type II fibers. Although Schoenfeld et al. [26] verified similar muscle hypertrophy in the soleus (predominance of type I fibers) and gastrocnemius (similar composition of type I and II fibers) to both load schemes (i.e., heavy and light) after 8 weeks of resistance training in untrained young subjects, factors such as the assessed muscles, the method used to assess hypertrophy (i.e., ultrasound), and training status make it difficult to extrapolate the results, meaning that further studies are required.
The results of this research also indicate that for resistance training with low loads has hypertrophic gains similar to high loads, yet the repetitions must be performed to muscular failure [14,21,22,23,28,39,42,43,45,46,47]. Recently, Lasevicius et al. [21] submitted 25 untrained males to four unilateral knee extension protocols. The high-load protocols with and without failure were performed at 80% 1RM, while the low-load protocols that were performed in the same manner were performed at 30% 1RM. The results of this study showed that when resistance training was performed with a high load, muscle failure did not seem to be a fundamental factor of achieving muscle hypertrophy. On the other hand, when the training protocol was performed to failure with a low load, it led to greater hypertrophy (7.8%) than the one that was not performed to failure (2.8%). According to the size principle proposed by Henneman et al. [9], motor units are recruited from the smallest to largest ones (i.e., as the strength demands increase, motor units with a greater excitability threshold are added to the motor units with smaller excitability threshold to increase force). In this regard, when resistance training with low loads is conducted to muscle failure, as the repetitions are performed, fatigue increases the effort required to produce the same force [57]. In this way, motor unit recruitment is increased until the entire motor unit pool is fully activated. This may be why resistance training with high and low loads leads to similar muscular hypertrophy. Furthermore, it is conceivable that resistance training with low loads that are performed to muscle failure would result in a long time under tension [25,58], which would cause greater metabolic stress [57], in addition to the slow speed of movement caused by fatigue, which could increase the mechanical tension. This mechanism would promote changes in metabolites that have anabolic signaling proper ties (e.g., lactate, α-ketoglutarate, and phosphatidic acid), which could potentiate muscle hypertrophy in the long term [59].
Although a wide spectrum of loads can be used during resistance training when aiming at muscle hypertrophy, there seems to be a minimal load threshold for resistance training to have hypertrophic responses that are similar to training with high loads. In this line, Lasevicius et al. [22] found that when low and high loads of resistance training were performed with matched volumes, the load schemes at 40, 60, and 80% 1RM were effective at increasing muscle hypertrophy without differences between them. However, 20% 1RM proved to be a suboptimal load to induce muscle hypertrophy compared to the other loads. There is probably a minimal load threshold (% 1RM) below which the applied mechanical tension may be insufficient to mediate the hypertrophic gains; nevertheless, this threshold has not been established [22]. To date, according to the studies that were selected for this systematic review, it is possible to infer that resistance training protocols should be designed to maximize metabolic stress (i.e., use of low and moderate loads), as well as the mechanical tension (i.e., use of high loads) to reach the entire load spectrum that is directed at muscle hypertrophy. Indeed, there is no doubt that a certain degree of mechanical tension may be achieved with low loads through mechanisms such as progressive fatigue-induced increase in motor unit recruitment, as well as decreases in movement speed that increase tension on individual sarcomeres. Nevertheless, the optimal load percentage (% 1RM) that is required for low-load resistance training to induce mechanical tension comparable to high loads has not yet been established. Perhaps the best way to achieve significant metabolic stress and mechanical tension simultaneously is to use moderate loads (i.e., between 67 and 85% 1RM or 6–12 repetitions) performed to muscular failure at some point in the season; however, this should be investigated in future studies.

5. Conclusions

Regarding maximal strength development, the main results of this systematic review suggest that high loads promote greater improvements compared to low loads for healthy adult males. These results are probably due to the specificity transfer of the load, as well as the 1RM test that is used to evaluate the individuals. When specific factors are eliminated and an isometric test is used, these differences are attenuated. However, as individuals become experienced in resistance training, higher training loads must be prioritized.
For muscle hypertrophy, studies indicate that a wide spectrum of loads (i.e., 30 to 90% 1RM) may be used for untrained male adult individuals. Although the same trend was observed in trained healthy adult males, the limited number of studies make it difficult to extrapolate the results to this population. Furthermore, muscle failure seems to be an important component for hypertrophic gains to be achieved. Nevertheless, coaches should be aware of the fact that resistance training that is performed with low loads until muscle failure might be quite demanding for joints and tendons, possibly increasing the risks of overtraining. On the other hand, training with high loads may reduce the total training volume and hinder hypertrophic gains. Thus, it seems that alternating periods with low and high loads, in addition to moderate loads are a good strategy to ensure the continuity of adaptive processes. Furthermore, lowloads (i.e., <30% 1RM) may be inefficient to improve muscle hypertrophy, even if they are driven to muscle failure. Therefore, we suggest that future studies compare training loads below 30% 1RM with moderate and high loads to clarify the training threshold at which low loads become ineffective for muscle hypertrophy.

Supplementary Materials

The following are available online at https://0-www-mdpi-com.brum.beds.ac.uk/article/10.3390/ijerph182111237/s1, Search strategy syntax.

Author Contributions

Conceptualization, M.L., J.G.V., Y.C., J.N., J.V. and M.W.; methodology; J.G.V., Y.C. and J.E.F.; software, M.L. and D.S.; validation, J.N., J.V. and M.W.; formal analysis, J.E.F.; investigation, M.L., D.S. and J.G.V.; resources, R.T. and M.W.; data curation, R.T. and J.E.F.; writing—original draft preparation, M.L., J.G.V. and Y.C.; writing—review and editing, M.L., J.G.V. and Y.C.; visualization, R.T.; supervision, J.N., J.V. and M.W.; project administration, M.L., J.G.V. and Y.C.; funding acquisition, J.G.V., Y.C., R.T. and M.W. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brasil (CAPES), finance code 001.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data presented in this study are available on request from the corresponding author.

Acknowledgments

Postgraduate Program in Physical Education, Federal University of Juiz de Fora (UFJF).

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Kraemer, W.J.; Ratamess, N.A.; Flanagan, S.D.; Shurley, J.P.; Todd, J.S.; Todd, T.C. Understanding the science of resistance training: An evolutionary perspective. Sports Med. 2017, 47, 2415–2435. [Google Scholar] [CrossRef]
  2. Ashton, R.E.; Tew, G.A.; Aning, J.J.; Gilbert, S.E.; Lewis, L.; Saxton, J.M. Effects of short-term, medium-term and long-term resistance exercise training on cardiometabolic health outcomes in adults: Systematic review with meta-analysis. Br. J. Sports Med. 2020, 54, 341–348. [Google Scholar] [CrossRef] [PubMed]
  3. Suchomel, T.J.; Nimphius, S.; Bellon, C.R.; Stone, M.H. The importance of muscular strength: Training considerations. Sports Med. 2018, 48, 765–785. [Google Scholar] [CrossRef]
  4. Westcott, W.L. Resistance training is medicine: Effects of strength training on health. Curr. Sports Med. Rep. 2012, 11, 209–216. [Google Scholar] [CrossRef]
  5. ACSM. American College of Sports Medicine position stand. Progression models in resistance training for healthy adults. Med. Sci. Sports Exerc. 2009, 41, 687–708. [Google Scholar] [CrossRef]
  6. Kraemer, W.J.; Ratamess, N.A. Fundamentals of resistance training: Progression and exercise prescription. Med. Sci. Sports Exerc. 2004, 36, 674–688. [Google Scholar] [CrossRef] [PubMed]
  7. NSCA. Essentials of Strength Training and Conditioning, 4th ed.; Human Kinetics: Champaign, IL, USA, 2016. [Google Scholar]
  8. Schoenfeld, B.J.; Grgic, J.; Van Every, D.W.; Plotkin, D.L. Loading Recommendations for Muscle Strength, Hypertrophy, and Local Endurance: A Re-Examination of the Repetition Continuum. Sports 2021, 9, 32. [Google Scholar] [CrossRef] [PubMed]
  9. Henneman, E.; Somjen, G.; Carpenter, D.O. Excitability and inhibitability of motoneurons of different sizes. J. Neurophysiol. 1965, 28, 599–620. [Google Scholar] [CrossRef]
  10. Coffey, T.H. Delorme method of restoration of muscle power by heavy resistance exercises. Treat. Serv. Bull. Can. Dep. Veterans’ Aff. 1946, 1, 8–11. [Google Scholar]
  11. Anderson, T.; Kearney, J.T. Effects of three resistance training programs on muscular strength and absolute and relative endurance. Res. Q. Exerc. Sport 1982, 53, 1–7. [Google Scholar] [CrossRef]
  12. Stone, W.J.; Coulter, S.P. Strength/endurance effects from three resistance training protocols with women. J. Strength Cond. Res. 1994, 8, 231–234. [Google Scholar]
  13. Lopes, C.R.; Aoki, M.S.; Crisp, A.H.; de Mattos, R.S.; Lins, M.A.; da Mota, G.R.; Schoenfeld, B.J.; Marchetti, P.H. The effect of different resistance training load schemes on strength and body composition in trained men. J. Hum. Kinet. 2017, 58, 177–186. [Google Scholar] [CrossRef] [Green Version]
  14. Nóbrega, S.R.; Ugrinowitsch, C.; Pintanel, L.; Barcelos, C.; Libardi, C.A. Effect of resistance training to muscle failure vs. volitional interruption at high- and low-intensities on muscle mass and strength. J. Strength Cond. Res. 2018, 32, 162–169. [Google Scholar] [CrossRef]
  15. Tanimoto, M.; Arakawa, H.; Sanada, K.; Miyachi, M.; Ishii, N. Changes in muscle activation and force generation patterns during cycling movements because of low-intensity squat training with slow movement and tonic force generation. J. Strength Cond. Res. 2009, 23, 2367–2376. [Google Scholar] [CrossRef]
  16. Tanimoto, M.; Ishii, N. Effects of low-intensity resistance exercise with slow movement and tonic force generation on muscular function in young men. J. Appl. Physiol. 2006, 100, 1150–1157. [Google Scholar] [CrossRef] [Green Version]
  17. Tanimoto, M.; Sanada, K.; Yamamoto, K.; Kawano, H.; Gando, Y.; Tabata, I.; Ishii, N.; Miyachi, M. Effects of whole-body low-intensity resistance training with slow movement and tonic force generation on muscular size and strength in young men. J. Strength Cond. Res. 2008, 22, 1926–1938. [Google Scholar] [CrossRef]
  18. Schoenfeld, B.J.; Fisher, J.P.; Grgic, J.; Haun, C.T.; Helms, E.T.; Phillips, S.M.; Steele, J.; Vigotsky, A.D. Resistance training recommendations to maximize muscle hypertrophy in an athletic population: Position stand of the IUSCA. Int. J. Strength Cond. 2021, 1, 1–30. [Google Scholar] [CrossRef]
  19. Lopez, P.; Radaelli, R.; Taaffe, D.R.; Newton, R.U.; Galvão, D.A.; Trajano, G.S.; Teodoro, J.L.; Kraemer, W.J.; Häkkinen, K.; Pinto, R.S. Resistance Training Load Effects on Muscle Hypertrophy and Strength Gain: Systematic Review and Network Meta-analysis. Med. Sci. Sports Exerc. 2021, 53, 1206–1216. [Google Scholar] [CrossRef]
  20. Burd, N.A.; Mitchell, C.J.; Churchward-Venne, T.A.; Phillips, S.M. Bigger weights may not beget bigger muscles: Evidence from acute muscle protein synthetic responses after resistance exercise. Appl. Physiol. Nutr. Metab. 2012, 37, 551–554. [Google Scholar] [CrossRef]
  21. Lasevicius, T.; Schoenfeld, B.J.; Silva-Batista, C.; Barros, T.S.; Aihara, A.Y.; Brendon, H.; Longo, A.R.; Tricoli, V.; Peres, B.A.; Teixeira, E.L. Muscle failure promotes greater muscle hypertrophy in low-load but not in high-load resistance training. J. Strength Cond. Res. 2019. [Google Scholar] [CrossRef]
  22. Lasevicius, T.; Ugrinowitsch, C.; Schoenfeld, B.J.; Roschel, H.; Tavares, L.D.; De Souza, E.O.; Laurentino, G.; Tricoli, V. Effects of different intensities of resistance training with equated volume load on muscle strength and hypertrophy. Eur. J. Sport Sci. 2018, 18, 772–780. [Google Scholar] [CrossRef]
  23. Lim, C.; Kim, H.J.; Morton, R.W.; Harris, R.; Phillips, S.M.; Jeong, T.S.; Kim, C.K. Resistance exercise-induced changes in muscle phenotype are load dependent. Med. Sci. Sports Exerc. 2019, 51, 2578–2585. [Google Scholar] [CrossRef]
  24. Schoenfeld, B.J.; Grgic, J.; Ogborn, D.; Krieger, J.W. Strength and hypertrophy adaptations between low- vs. high-load resistance training: A systematic review and meta-analysis. J. Strength Cond. Res. 2017, 31, 3508–3523. [Google Scholar] [CrossRef]
  25. Grgic, J.; Homolak, J.; Mikulic, P.; Botella, J.; Schoenfeld, B.J. Inducing hypertrophic effects of type I skeletal muscle fibers: A hypothetical role of time under load in resistance training aimed at muscular hypertrophy. Med. Hypotheses 2018, 112, 40–42. [Google Scholar] [CrossRef] [Green Version]
  26. Schoenfeld, B.J.; Vigotsky, A.D.; Grgic, J.; Haun, C.; Contreras, B.; Delcastillo, K.; Francis, A.; Cote, G.; Alto, A. Do the anatomical and physiological properties of a muscle determine its adaptive response to different loading protocols? Physiol. Rep. 2020, 8, e14427. [Google Scholar] [CrossRef]
  27. Grgic, J. The effects of low-load vs. high-load resistance training on muscle fiber hypertrophy: A meta-analysis. J. Hum. Kinet. 2020, 74, 51–58. [Google Scholar] [CrossRef]
  28. Mitchell, C.J.; Churchward-Venne, T.A.; West, D.W.; Burd, N.A.; Breen, L.; Baker, S.K.; Phillips, S.M. Resistance exercise load does not determine training-mediated hypertrophic gains in young men. J. Appl. Physiol. 2012, 113, 71–77. [Google Scholar] [CrossRef] [Green Version]
  29. Campos, G.E.; Luecke, T.J.; Wendeln, H.K.; Toma, K.; Hagerman, F.C.; Murray, T.F.; Ragg, K.E.; Ratamess, N.A.; Kraemer, W.J.; Staron, R.S. Muscular adaptations in response to three different resistance-training regimens: Specificity of repetition maximum training zones. Eur. J. Appl. Physiol. 2002, 88, 50–60. [Google Scholar] [CrossRef]
  30. Schoenfeld, B.J.; Wilson, J.M.; Lowery, R.P.; Krieger, J.W. Muscular adaptations in low- versus high-load resistance training: A meta-analysis. Eur. J. Sport Sci. 2016, 16, 1–10. [Google Scholar] [CrossRef]
  31. Liberati, A.; Altman, D.G.; Tetzlaff, J.; Mulrow, C.; Gøtzsche, P.C.; Ioannidis, J.P.; Clarke, M.; Devereaux, P.J.; Kleijnen, J.; Moher, D. The PRISMA statement for reporting systematic reviews and meta-analyses of studies that evaluate health care interventions: Explanation and elaboration. PLoS Med. 2009, 6, e1000100. [Google Scholar] [CrossRef]
  32. Page, M.J.; McKenzie, J.E.; Bossuyt, P.M.; Boutron, I.; Hoffmann, T.C.; Mulrow, C.D.; Shamseer, L.; Tetzlaff, J.M.; Akl, E.A.; Brennan, S.E.; et al. The PRISMA 2020 statement: An updated guideline for reporting systematic reviews. PLoS Med. 2021, 18, e1003583. [Google Scholar] [CrossRef]
  33. Brown, P.; Brunnhuber, K.; Chalkidou, K.; Chalmers, I.; Clarke, M.; Fenton, M.; Forbes, C.; Glanville, J.; Hicks, N.J.; Moody, J.; et al. How to formulate research recommendations. BMJ 2006, 333, 804–806. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  34. Rhea, M.R. Determining the magnitude of treatment effects in strength training research through the use of the effect size. J. Strength Cond. Res. 2004, 18, 918–920. [Google Scholar] [CrossRef] [PubMed]
  35. Elkins, M.R.; Herbert, R.D.; Moseley, A.M.; Sherrington, C.; Maher, C. Rating the quality of trials in systematic reviews of physical therapy interventions. Cardiopulm. Phys. Ther. J. 2010, 21, 20–26. [Google Scholar] [CrossRef]
  36. Moseley, A.M.; Herbert, R.D.; Sherrington, C.; Maher, C.G. Evidence for physiotherapy practice: A survey of the Physiotherapy Evidence Database (PEDro). Aust. J. Physiother. 2002, 48, 43–49. [Google Scholar] [CrossRef] [Green Version]
  37. Valkenet, K.; van de Port, I.G.; Dronkers, J.J.; de Vries, W.R.; Lindeman, E.; Backx, F.J. The effects of preoperative exercise therapy on postoperative outcome: A systematic review. Clin. Rehabil. 2011, 25, 99–111. [Google Scholar] [CrossRef]
  38. Fink, J.; Kikuchi, N.; Nakazato, K. Effects of rest intervals and training loads on metabolic stress and muscle hypertrophy. Clin. Physiol. Funct. Imaging 2016, 38, 261–268. [Google Scholar] [CrossRef] [PubMed]
  39. Fink, J.; Kikuchi, N.; Yoshida, S.; Terada, K.; Nakazato, K. Impact of high versus low fixed loads and non-linear training loads on muscle hypertrophy, strength and force development. SpringerPlus 2016, 5, 698. [Google Scholar] [CrossRef] [Green Version]
  40. Fisher, J.P.; Ironside, M.; Steele, J. Heavier and lighter load resistance training to momentary failure produce similar increases in strength with differing degrees of discomfort. Muscle Nerve 2017, 56, 797–803. [Google Scholar] [CrossRef] [PubMed]
  41. Holm, L.; Reitelseder, S.; Pedersen, T.G.; Doessing, S.; Petersen, S.G.; Flyvbjerg, A.; Andersen, J.L.; Aagaard, P.; Kjaer, M. Changes in muscle size and MHC composition in response to resistance exercise with heavy and light loading intensity. J. Appl. Physiol. 2008, 105, 1454–1461. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  42. Jenkins, N.D.; Housh, T.J.; Buckner, S.L.; Bergstrom, H.C.; Cochrane, K.C.; Hill, E.C.; Smith, C.M.; Schmidt, R.J.; Johnson, G.O.; Cramer, J.T. Neuromuscular adaptations after 2 and 4 weeks of 80% versus 30% 1 repetition maximum resistance training to failure. J. Strength Cond. Res. 2016, 30, 2174–2185. [Google Scholar] [CrossRef] [PubMed]
  43. Jenkins, N.D.M.; Miramonti, A.A.; Hill, E.C.; Smith, C.M.; Cochrane-Snyman, K.C.; Housh, T.J.; Cramer, J.T. Greater neural adaptations following high- vs. low-load resistance training. Front. Physiol. 2017, 8, 331. [Google Scholar] [CrossRef]
  44. Netreba, A.; Popov, D.; Bravyy, Y.; Lyubaeva, E.; Terada, M.; Ohira, T.; Okabe, H.; Vinogradova, O.; Ohira, Y. Responses of knee extensor muscles to leg press training of various types in human. Ross. Fiziol. Zhurnal Im. IM Sechenova 2013, 99, 406–416. [Google Scholar]
  45. Popov, D.V.; Tsvirkun, D.V.; Netreba, A.I.; Tarasova, O.S.; Prostova, A.B.; Larina, I.M.; Borovik, A.S.; Vinogradova, O.L. Hormonal adaptation determines the increase in muscle mass and strength during low-intensity strength training without relaxation. Fiziol. Cheloveka 2006, 32, 121–127. [Google Scholar] [CrossRef]
  46. Morton, R.W.; Oikawa, S.Y.; Wavell, C.G.; Mazara, N.; McGlory, C.; Quadrilatero, J.; Baechler, B.L.; Baker, S.K.; Phillips, S.M. Neither load nor systemic hormones determine resistance training-mediated hypertrophy or strength gains in resistance-trained young men. J. Appl. Physiol. 2016, 121, 129–138. [Google Scholar] [CrossRef] [Green Version]
  47. Schoenfeld, B.J.; Peterson, M.D.; Ogborn, D.; Contreras, B.; Sonmez, G.T. Effects of low- vs. high-load resistance training on muscle strength and hypertrophy in well-trained Men. J. Strength Cond. Res. 2015, 29, 2954–2963. [Google Scholar] [CrossRef] [Green Version]
  48. Jones, K.; Bishop, P.; Hunter, G.; Fleisig, G. The effects of varying resistance-training loads on intermediate- and high-velocity-specific adaptations. J. Strength Cond. Res. 2001, 15, 349–356. [Google Scholar]
  49. Sale, D.G. Neural adaptation to resistance training. Med. Sci. Sports Exerc. 1988, 20, S135–S145. [Google Scholar] [CrossRef]
  50. Moritani, T.; de Vries, H.A. Neural factors versus hypertrophy in the time course of muscle strength gain. Am. J. Phys. Med. 1979, 58, 115–130. [Google Scholar]
  51. Carroll, T.J.; Barry, B.; Riek, S.; Carson, R.G. Resistance training enhances the stability of sensorimotor coordination. Proc. Biol. Sci. 2001, 268, 221–227. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  52. Škarabot, J.; Brownstein, C.G.; Casolo, A.; Del Vecchio, A.; Ansdell, P. The knowns and unknowns of neural adaptations to resistance training. Eur. J. Appl. Physiol. 2021, 121, 675–685. [Google Scholar] [CrossRef] [PubMed]
  53. Balshaw, T.G.; Massey, G.J.; Maden-Wilkinson, T.M.; Folland, J.P. Muscle size and strength: Debunking the “completely separate phenomena” suggestion. Eur. J. Appl. Physiol. 2017, 117, 1275–1276. [Google Scholar] [CrossRef] [PubMed]
  54. Gabriel, D.A.; Kamen, G.; Frost, G. Neural adaptations to resistive exercise: Mechanisms and recommendations for training practices. Sports Med. 2006, 36, 133–149. [Google Scholar] [CrossRef]
  55. Aagaard, P.; Simonsen, E.B.; Andersen, J.L.; Magnusson, P.; Dyhre-Poulsen, P. Neural adaptation to resistance training: Changes in evoked V-wave and H-reflex responses. J. Appl. Physiol. 2002, 92, 2309–2318. [Google Scholar] [CrossRef]
  56. McCall, G.E.; Byrnes, W.C.; Dickinson, A.; Pattany, P.M.; Fleck, S.J. Muscle fiber hypertrophy, hyperplasia, and capillary density in college men after resistance training. J. Appl. Physiol. 1996, 81, 2004–2012. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  57. Grgic, J.; Schoenfeld, B.J. Are the hypertrophic adaptations to high and low-load resistance training muscle fiber type specific? Front. Physiol. 2018, 9, 402. [Google Scholar] [CrossRef] [Green Version]
  58. Ogborn, D.; Schoenfeld, B. The role of fiber types in muscle hypertrophy: Implications for loading strategies. Strength Cond. J. 2014, 36, 20–25. [Google Scholar] [CrossRef] [Green Version]
  59. Wackerhage, H.; Schoenfeld, B.J.; Hamilton, D.L.; Lehti, M.; Hulmi, J.J. Stimuli and sensors that initiate skeletal muscle hypertrophy following resistance exercise. J. Appl. Physiol. 2019, 126, 30–43. [Google Scholar] [CrossRef]
Figure 1. PRISMA 2020 flow diagram for this systematic review, which included searches of databases, registers, and other sources.
Figure 1. PRISMA 2020 flow diagram for this systematic review, which included searches of databases, registers, and other sources.
Ijerph 18 11237 g001
Table 1. PICOS eligibility criteria for the inclusion of studies in the systematic review.
Table 1. PICOS eligibility criteria for the inclusion of studies in the systematic review.
ParametersInclusion Criteria
ParticipantsHealthy males aged between 18 and 40 years; resistance trained or untrained; without a history of bone, muscle, or articular injury.
InterventionIntervention performed with low loads (<67% 1RM or >12 repetitions).
ComparatorsIntervention performed with moderate loads (67–85% 1RM or 6–12 repetitions) or high loads (>85% 1RM or <6 repetitions).
OutcomesDevelopment of maximal strength (dynamic strength or isometric strength) and muscle hypertrophy (cross-sectional area, muscle thickness, or skeletal muscle fiber size).
Study designExperimental randomized studies with between- or within-subject parallel designs.
Table 2. Characteristics of the participants.
Table 2. Characteristics of the participants.
StudyParticipants
(n = 563)
Age (years)Height (cm)Weight (kg)Training Status
Anderson and Kearney [11]—mean ± SD4320.7 ± 1.8180.0 ± 13.075.1 ± 3.9UT
Campos et al. [29]—mean ± SD3222.5 ± 5.8178.3 ± 7.277.8 ± 11.9UT
Fink et al. [38]—mean ± SD2019.8 ± 1.0169.3 ± 4.464.1 ± 7.9UT
Fink et al. [39]—mean ± SD2123.2 ± 2.7168.6 ± 4.563.4 ± 4.7UT
Fisher et al. [40]—mean ± SD720.6 ± 0.5178.9 ± 3.277.1 ± 2.7UT
Holm et al. [41]—mean ± SE1124.7 ± 1.1183.0 ± 2.079.7 ± 4.0UT
Jenkins et al. [42]—mean ± SD1521.7 ± 2.4181.6 ± 7.584.7 ± 23.5UT
Jenkins et al. [43]—mean ± SD2623.1 ± 4.7180.6 ± 6.080.0 ± 14.1UT
Jones et al. [48]—mean ± SD2620.1 ± 1.4183.1 ± 5.380.5 ± 11.1RT
Lasevicius et al. [22]—mean ± SD3024.5 ± 2.4180.0 ± 0.777.0 ± 16.5UT
Lasevicius et al. [21]—mean ± SD2524.0 ± 4.8176.0 ± 6.574.3 ± 12.6UT
Lim et al. [23]—mean ± SD2123.4 ± 1.7174.3 ± 6.576.7 ± 11.1UT
Lopes et al. [13]—mean ± SD1626.6 ± 6.1176.9 ± 6.281.5 ± 12.6RT
Mitchell et al. [28]—mean ± SE1821.0 ± 0.8176.0 ± 4.073.3 ± 1.4UT
Morton et al. [46]—mean ± SE4923.0 ± 1.0181.0 ± 1.086.0 ± 2.0RT
Netreba et al. [44]—mean ± SE3024.8 ± 3.1178.9 ± 6.474.4 ± 7.3UT
Nóbrega et al. [14]—mean ± SD32 (5 dropped out)23.0 ± 3.6176.0 ± 0.6Not reportedUT
Popov et al. [45]—mean ± SE1821.0 ± 2.0181.0 ± 10.075.0 ± 3.0UT
Schoenfeld et al. [47]—mean24 (6 dropped out)23.3175.082.5RT
Schoenfeld et al. [26]—mean30 (4 dropped out)22.5175.777.3UT
Tanimoto and Ishii [16]—mean ± SD2419.4 ± 0.7170.3 ± 4.859.4 ± 5.9UT
Tanimoto et al. [17]—mean ± SD3619.4 ± 0.7174.4 ± 5.663.5 ± 4.2UT
Tanimoto et al. [15]—mean ± SD2420.1 ± 1.1175.1 ± 5.762.7 ± 4.1UT
n: sample size; RT: recreationally trained; SD: standard deviation; SE: standard error; UT: untrained.
Table 4. Methodological quality of the included studies.
Table 4. Methodological quality of the included studies.
Study1234567891011TotalOverall Quality
Anderson and Kearney [11]YYNYNNNYYYY6High
Campos et al. [29]YYNYNNNYYYN5Moderate
Fink et al. [38]YYNYNNNYYYY6High
Fink et al. [39]YYNYNNNYNYY6High
Fisher et al. [40]YNNYNNNNYYY5Moderate
Holm et al. [41]YYNYNNYYYYY7High
Jenkins et al. [42]YYNYNNNNNYY4Moderate
Jenkins et al. [43]NYNYNNNYYYY6High
Jones et al. [48]YYNYNNNYYYY6High
Lasevicius et al. [22]YYNYNNNYYYY6High
Lasevicius et al. [21]YYNYNNNYYYY6High
Lim et al. [23]YYNYNNNYYYY6High
Lopes et al. [13]YYNYNNNYYYN6High
Mitchell et al. [28]YYNYNNNYYYY6High
Morton et al. [46]YYNYNNYNNYY5Moderate
Netreba et al. [44]NYNNNNNYYYY5Moderate
Nóbrega et al. [14]NYNYNNNNNYY4Moderate
Popov et al. [45]NNNYNNNYYYY5Moderate
Schoenfeld et al. [47]YYNYNNNYNYY5Moderate
Schoenfeld et al. [26]YYNYNYYYYYY8High
Tanimoto and Ishii [16]YYNYNNNYYYY6High
Tanimoto et al. [17]YYNYNNNYYYY6High
Tanimoto et al. [15]YYNYNNNYYYY6High
Mean-----------5.7 ± 0.9Moderate
Median-----------6.0-
Y: yes; N: no. PEDro scale criteria. 1: Eligibility criteria were specified. 2: Subjects were randomly allocated to groups (in a crossover study, subjects were randomly allocated an order in which treatments were received). 3: Allocation was concealed. 4: The groups were similar at baseline regarding most important prognostic indicators. 5: There was blinding of all subjects. 6: There was blinding of all therapists/researchers who administered the therapy/protocol. 7: There was blinding of all assessors who measured at least one key outcome. 8: Measures of at least one key outcome were obtained from more than 85% of the subjects that were initially allocated to groups. 9: All subjects for whom outcome measures were available received the treatment or control condition as allocated or, where this was not the case, data for at least one key outcome were analyzed using “intention to treat.” 10: The results of between-group statistical comparisons were reported for at least one key outcome. 11: The study provided both point measures and measures of variability for at least one key outcome.
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Lacio, M.; Vieira, J.G.; Trybulski, R.; Campos, Y.; Santana, D.; Filho, J.E.; Novaes, J.; Vianna, J.; Wilk, M. Effects of Resistance Training Performed with Different Loads in Untrained and Trained Male Adult Individuals on Maximal Strength and Muscle Hypertrophy: A Systematic Review. Int. J. Environ. Res. Public Health 2021, 18, 11237. https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph182111237

AMA Style

Lacio M, Vieira JG, Trybulski R, Campos Y, Santana D, Filho JE, Novaes J, Vianna J, Wilk M. Effects of Resistance Training Performed with Different Loads in Untrained and Trained Male Adult Individuals on Maximal Strength and Muscle Hypertrophy: A Systematic Review. International Journal of Environmental Research and Public Health. 2021; 18(21):11237. https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph182111237

Chicago/Turabian Style

Lacio, Marcio, João Guilherme Vieira, Robert Trybulski, Yuri Campos, Derick Santana, José Elias Filho, Jefferson Novaes, Jeferson Vianna, and Michal Wilk. 2021. "Effects of Resistance Training Performed with Different Loads in Untrained and Trained Male Adult Individuals on Maximal Strength and Muscle Hypertrophy: A Systematic Review" International Journal of Environmental Research and Public Health 18, no. 21: 11237. https://0-doi-org.brum.beds.ac.uk/10.3390/ijerph182111237

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop