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Article

Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study

1
Department of Operative Dentistry, Dental School (Carolinum), Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany
2
Department of Periodontology, School of Dental Medicine, Stony Brook University, Stony Brook, New York, NY 11794, USA
3
Department of Oral Surgery and Implant Dentistry, Dental School (Carolinum), Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany
4
Institute of Biostatistics and Mathematical Modelling, Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany
*
Author to whom correspondence should be addressed.
Received: 11 December 2019 / Revised: 13 January 2020 / Accepted: 15 January 2020 / Published: 17 January 2020
(This article belongs to the Collection Dental Biomaterials)
Six dentin adhesives were tested in vitro regarding their cytotoxicity on human fibroblasts. The adhesives Hybrid Bond, One-up Bond F Plus, AdheSE, Clearfil SE Bond, Optibond Solo Plus and Syntac were eluted with culture medium as single or sequentially applied adhesive part for 24 h. 75 Petri dishes were produced per group. They were evaluated triangulated, comprising the quantitative evaluation (105 ones) to determine “viable”, “dead” and “debris” cells with the use of a cell-counter and the reactivity index was also identified based on the qualitative assessment (420 ones). One-up Bond F Plus, AdheSE and Clearfil SE Bond showed a statistical difference of viable cells to the cell control. For One-up Bond F Plus, statistically, differences compared to hybrid bond and Syntac were also found. All the adhesives except One-up Bond F Plus showed significant differences between single and sequentially applied adhesive part regarding the quantitative evaluation. The test material showed a moderate grade of cytotoxicity. As a result, a statistically significant difference of the cytotoxicity between the self-etch and etch-and-rinse adhesives cannot be demonstrated regarding the qualitative evaluation and the reactivity index, but the differences between sequentially applied and single applied components can be proved. View Full-Text
Keywords: cells; cytotoxicity; dentin adhesives; fibroblasts; in vitro; screening cells; cytotoxicity; dentin adhesives; fibroblasts; in vitro; screening
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MDPI and ACS Style

Fröb, L.; Rüttermann, S.; Romanos, G.E.; Herrmann, E.; Gerhardt-Szép, S. Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study. Materials 2020, 13, 452. https://0-doi-org.brum.beds.ac.uk/10.3390/ma13020452

AMA Style

Fröb L, Rüttermann S, Romanos GE, Herrmann E, Gerhardt-Szép S. Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study. Materials. 2020; 13(2):452. https://0-doi-org.brum.beds.ac.uk/10.3390/ma13020452

Chicago/Turabian Style

Fröb, Luisa, Stefan Rüttermann, Georgios E. Romanos, Eva Herrmann, and Susanne Gerhardt-Szép. 2020. "Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study" Materials 13, no. 2: 452. https://0-doi-org.brum.beds.ac.uk/10.3390/ma13020452

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