Next Article in Journal
Characterization of Emerging Swine Viral Diseases through Oxford Nanopore Sequencing Using Senecavirus A as a Model
Next Article in Special Issue
Superimposition of Viral Protein Structures: A Means to Decipher the Phylogenies of Viruses
Previous Article in Journal
Bovine Pestivirus Heterogeneity and Its Potential Impact on Vaccination and Diagnosis
Previous Article in Special Issue
Perspectives on Viral RNA Genomes and the RNA Folding Problem
Article

The Central Spike Complex of Bacteriophage T4 Contacts PpiD in the Periplasm of Escherichia coli

1
Institute of Biology, University of Hohenheim, 190h, Garbenstr. 30, 70599 Stuttgart, Germany
2
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Laboratory of Molecular Bioengineering, 16/10 Miklukho-Maklaya St., 117997 Moscow, Russia
3
Department of BMB, Basic Sciences Building 6.600D, University of Texas Medical Branch, 301 University Blvd, Galveston, TX 77555-0647, USA
*
Author to whom correspondence should be addressed.
Received: 4 September 2020 / Revised: 1 October 2020 / Accepted: 2 October 2020 / Published: 6 October 2020
(This article belongs to the Special Issue In Memory of Michael Rossmann)
Infecting bacteriophage T4 uses a contractile tail structure to breach the envelope of the Escherichia coli host cell. During contraction, the tail tube headed with the “central spike complex” is thought to mechanically puncture the outer membrane. We show here that a purified tip fragment of the central spike complex interacts with periplasmic chaperone PpiD, which is anchored to the cytoplasmic membrane. PpiD may be involved in the penetration of the inner membrane by the T4 injection machinery, resulting in a DNA-conducting channel to translocate the phage DNA into the interior of the cell. Host cells with the ppiD gene deleted showed partial reduction in the plating efficiency of T4, suggesting a supporting role of PpiD to improve the efficiency of the infection process. View Full-Text
Keywords: bacteriophage T4; tail structure; periplasmic chaperone; DNA translocation bacteriophage T4; tail structure; periplasmic chaperone; DNA translocation
Show Figures

Figure 1

MDPI and ACS Style

Wenzel, S.; Shneider, M.M.; Leiman, P.G.; Kuhn, A.; Kiefer, D. The Central Spike Complex of Bacteriophage T4 Contacts PpiD in the Periplasm of Escherichia coli. Viruses 2020, 12, 1135. https://0-doi-org.brum.beds.ac.uk/10.3390/v12101135

AMA Style

Wenzel S, Shneider MM, Leiman PG, Kuhn A, Kiefer D. The Central Spike Complex of Bacteriophage T4 Contacts PpiD in the Periplasm of Escherichia coli. Viruses. 2020; 12(10):1135. https://0-doi-org.brum.beds.ac.uk/10.3390/v12101135

Chicago/Turabian Style

Wenzel, Sabrina, Mikhail M. Shneider, Petr G. Leiman, Andreas Kuhn, and Dorothee Kiefer. 2020. "The Central Spike Complex of Bacteriophage T4 Contacts PpiD in the Periplasm of Escherichia coli" Viruses 12, no. 10: 1135. https://0-doi-org.brum.beds.ac.uk/10.3390/v12101135

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop