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LysSAP26, a New Recombinant Phage Endolysin with a Broad Spectrum Antibacterial Activity

Department of Microbiology, School of Medicine, Kyungpook National University, Daegu 41944, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 23 October 2020 / Revised: 18 November 2020 / Accepted: 19 November 2020 / Published: 23 November 2020
(This article belongs to the Section Bacterial Viruses)
Multidrug-resistant (MDR) bacteria are a major threat to public health. Bacteriophage endolysins (lysins) are a promising alternative treatment to traditional antibiotics. However, the lysins currently under development are still underestimated. Herein, we cloned the lysin from the SAP-26 bacteriophage genome. The recombinant LysSAP26 protein inhibited the growth of carbapenem-resistant Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa, oxacillin-resistant Staphylococcus aureus, and vancomycin-resistant Enterococcus faecium with minimum inhibitory concentrations of 5~80 µg/mL. In animal experiments, mice infected with A. baumannii were protected by LysSAP26, with a 40% survival rate. Transmission electron microscopy analysis confirmed that LysSAP26 treatment resulted in the destruction of bacterial cell walls. LysSAP26 is a new endolysin that can be applied to treat MDR A. baumannii, E. faecium, S. aureus, K. pneumoniae, P. aeruginosa, and E. coli infections, targeting both Gram-positive and Gram-negative bacteria. View Full-Text
Keywords: endolysin; multidrug-resistant bacteria; antimicrobial activity endolysin; multidrug-resistant bacteria; antimicrobial activity
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MDPI and ACS Style

Kim, S.; Jin, J.-S.; Choi, Y.-J.; Kim, J. LysSAP26, a New Recombinant Phage Endolysin with a Broad Spectrum Antibacterial Activity. Viruses 2020, 12, 1340. https://0-doi-org.brum.beds.ac.uk/10.3390/v12111340

AMA Style

Kim S, Jin J-S, Choi Y-J, Kim J. LysSAP26, a New Recombinant Phage Endolysin with a Broad Spectrum Antibacterial Activity. Viruses. 2020; 12(11):1340. https://0-doi-org.brum.beds.ac.uk/10.3390/v12111340

Chicago/Turabian Style

Kim, Shukho, Jong-Sook Jin, Yoon-Jung Choi, and Jungmin Kim. 2020. "LysSAP26, a New Recombinant Phage Endolysin with a Broad Spectrum Antibacterial Activity" Viruses 12, no. 11: 1340. https://0-doi-org.brum.beds.ac.uk/10.3390/v12111340

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