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Article

Comparison of Rapid Antigen Tests for COVID-19

1
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
2
Division of Infectious Diseases, Advanced Clinical Research Center, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
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Department of Infectious Diseases and Applied Immunology, IMSUT Hospital of Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
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Emergency Medical Center, Fujisawa City Hospital, Kanagawa 251-8550, Japan
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Pulmonary Division, Department of Internal Medicine, Saiseikai Utsunomiya Hospital, Tochigi 321-0974, Japan
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Division of Pulmonary Medicine, Department of Internal Medicine, Tachikawa Hospital, Tokyo 190-8531, Japan
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Department of Refractory Viral Infections, National Center for Global Health and Medicine Research Institute, Tokyo 162-8655, Japan
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Department of Emergency Medicine and Critical Care Medicine, Saiseikai Utsunomiya Hospital, Tochigi 321-0974, Japan
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Disease Control and Prevention Center, National Center for Global Health and Medicine Hospital, Tokyo 162-8655, Japan
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Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA
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International Research Center for Infectious Diseases, Department of Special Pathogens, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan
*
Authors to whom correspondence should be addressed.
Academic Editor: Giorgio Palù
Received: 12 November 2020 / Revised: 28 November 2020 / Accepted: 8 December 2020 / Published: 10 December 2020
(This article belongs to the Section SARS-CoV-2 and COVID-19)
Reverse transcription-quantitative PCR (RT-qPCR)-based tests are widely used to diagnose coronavirus disease 2019 (COVID-19). As a result that these tests cannot be done in local clinics where RT-qPCR testing capability is lacking, rapid antigen tests (RATs) for COVID-19 based on lateral flow immunoassays are used for rapid diagnosis. However, their sensitivity compared with each other and with RT-qPCR and infectious virus isolation has not been examined. Here, we compared the sensitivity among four RATs by using severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) isolates and several types of COVID-19 patient specimens and compared their sensitivity with that of RT-qPCR and infectious virus isolation. Although the RATs read the samples containing large amounts of virus as positive, even the most sensitive RAT read the samples containing small amounts of virus as negative. Moreover, all RATs tested failed to detect viral antigens in several specimens from which the virus was isolated. The current RATs will likely miss some COVID-19 patients who are shedding infectious SARS-CoV-2. View Full-Text
Keywords: rapid antigen test; diagnosis; COVID-19; SARS-CoV-2 rapid antigen test; diagnosis; COVID-19; SARS-CoV-2
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Figure 1

MDPI and ACS Style

Yamayoshi, S.; Sakai-Tagawa, Y.; Koga, M.; Akasaka, O.; Nakachi, I.; Koh, H.; Maeda, K.; Adachi, E.; Saito, M.; Nagai, H.; Ikeuchi, K.; Ogura, T.; Baba, R.; Fujita, K.; Fukui, T.; Ito, F.; Hattori, S.-i.; Yamamoto, K.; Nakamoto, T.; Furusawa, Y.; Yasuhara, A.; Ujie, M.; Yamada, S.; Ito, M.; Mitsuya, H.; Omagari, N.; Yotsuyanagi, H.; Iwatsuki-Horimoto, K.; Imai, M.; Kawaoka, Y. Comparison of Rapid Antigen Tests for COVID-19. Viruses 2020, 12, 1420. https://0-doi-org.brum.beds.ac.uk/10.3390/v12121420

AMA Style

Yamayoshi S, Sakai-Tagawa Y, Koga M, Akasaka O, Nakachi I, Koh H, Maeda K, Adachi E, Saito M, Nagai H, Ikeuchi K, Ogura T, Baba R, Fujita K, Fukui T, Ito F, Hattori S-i, Yamamoto K, Nakamoto T, Furusawa Y, Yasuhara A, Ujie M, Yamada S, Ito M, Mitsuya H, Omagari N, Yotsuyanagi H, Iwatsuki-Horimoto K, Imai M, Kawaoka Y. Comparison of Rapid Antigen Tests for COVID-19. Viruses. 2020; 12(12):1420. https://0-doi-org.brum.beds.ac.uk/10.3390/v12121420

Chicago/Turabian Style

Yamayoshi, Seiya; Sakai-Tagawa, Yuko; Koga, Michiko; Akasaka, Osamu; Nakachi, Ichiro; Koh, Hidefumi; Maeda, Kenji; Adachi, Eisuke; Saito, Makoto; Nagai, Hiroyuki; Ikeuchi, Kazuhiko; Ogura, Takayuki; Baba, Rie; Fujita, Kensuke; Fukui, Takahiro; Ito, Fumimaro; Hattori, Shin-ichiro; Yamamoto, Kei; Nakamoto, Takato; Furusawa, Yuri; Yasuhara, Atsuhiro; Ujie, Michiko; Yamada, Shinya; Ito, Mutsumi; Mitsuya, Hiroaki; Omagari, Norio; Yotsuyanagi, Hiroshi; Iwatsuki-Horimoto, Kiyoko; Imai, Masaki; Kawaoka, Yoshihiro. 2020. "Comparison of Rapid Antigen Tests for COVID-19" Viruses 12, no. 12: 1420. https://0-doi-org.brum.beds.ac.uk/10.3390/v12121420

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