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Review

Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2

1
Biomedical Research Center, Qatar University, P.O. Box 2713 Doha, Qatar
2
College of Medicine, Member of QU Health, Qatar University, P.O. Box 2713 Doha, Qatar
3
Department of Biomedical Science, College of Health Sciences, Member of QU Health, Qatar University, P.O. Box 2713 Doha, Qatar
4
College of Dental Medicine, Member of QU Health, Qatar University, P.O. Box 2713 Doha, Qatar
*
Author to whom correspondence should be addressed.
Received: 27 April 2020 / Revised: 19 May 2020 / Accepted: 20 May 2020 / Published: 26 May 2020
(This article belongs to the Special Issue Novel Concepts in Virology)
The recent outbreak of the Coronavirus disease 2019 (COVID-19) has quickly spread worldwide since its discovery in Wuhan city, China in December 2019. A comprehensive strategy, including surveillance, diagnostics, research, clinical treatment, and development of vaccines, is urgently needed to win the battle against COVID-19. The past three unprecedented outbreaks of emerging human coronavirus infections at the beginning of the 21st century have highlighted the importance of readily available, accurate, and rapid diagnostic technologies to contain emerging and re-emerging pandemics. Real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) based assays performed on respiratory specimens remain the gold standard for COVID-19 diagnostics. However, point-of-care technologies and serologic immunoassays are rapidly emerging with high sensitivity and specificity as well. Even though excellent techniques are available for the diagnosis of symptomatic patients with COVID-19 in well-equipped laboratories; critical gaps still remain in screening asymptomatic people who are in the incubation phase of the virus, as well as in the accurate determination of live viral shedding during convalescence to inform decisions for ending isolation. This review article aims to discuss the currently available laboratory methods and surveillance technologies available for the detection of COVID-19, their performance characteristics and highlight the gaps in current diagnostic capacity, and finally, propose potential solutions. We also summarize the specifications of the majority of the available commercial kits (PCR, EIA, and POC) for laboratory diagnosis of COVID-19. View Full-Text
Keywords: COVID-19; SARS-CoV-2; viruses; diagnostic challenges; molecular testing; serology COVID-19; SARS-CoV-2; viruses; diagnostic challenges; molecular testing; serology
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MDPI and ACS Style

Younes, N.; Al-Sadeq, D.W.; AL-Jighefee, H.; Younes, S.; Al-Jamal, O.; Daas, H.I.; Yassine, H.M.; Nasrallah, G.K. Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2. Viruses 2020, 12, 582. https://0-doi-org.brum.beds.ac.uk/10.3390/v12060582

AMA Style

Younes N, Al-Sadeq DW, AL-Jighefee H, Younes S, Al-Jamal O, Daas HI, Yassine HM, Nasrallah GK. Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2. Viruses. 2020; 12(6):582. https://0-doi-org.brum.beds.ac.uk/10.3390/v12060582

Chicago/Turabian Style

Younes, Nadin; Al-Sadeq, Duaa W.; AL-Jighefee, Hadeel; Younes, Salma; Al-Jamal, Ola; Daas, Hanin I.; Yassine, Hadi. M.; Nasrallah, Gheyath K. 2020. "Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2" Viruses 12, no. 6: 582. https://0-doi-org.brum.beds.ac.uk/10.3390/v12060582

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