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Brief Report

Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples

Unité des Virus Émergents (UVE: Aix-Marseille Univ-IRD 190-Inserm 1207-IHU Méditerranée Infection), 13005 Marseille, France
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Author to whom correspondence should be addressed.
Received: 24 April 2020 / Revised: 3 June 2020 / Accepted: 7 June 2020 / Published: 8 June 2020
(This article belongs to the Special Issue Pathogenesis of Human and Animal Coronaviruses)
Clinical samples collected in coronavirus disease 19 (COVID-19), patients are commonly manipulated in biosafety level 2 laboratories for molecular diagnostic purposes. Here, we tested French norm NF-EN-14476+A2 derived from European standard EN-14885 to assess the risk of manipulating infectious viruses prior to RNA extraction. SARS-CoV-2 cell-culture supernatant and nasopharyngeal samples (virus-spiked samples and clinical samples collected in COVID-19 patients) were used to measure the reduction of infectivity after 10 min contact with lysis buffer containing various detergents and chaotropic agents. A total of thirteen protocols were evaluated. Two commercially available formulations showed the ability to reduce infectivity by at least 6 log 10, whereas others proved less effective. View Full-Text
Keywords: SARS-CoV-2; coronavirus; extraction buffer; COVID-19; inactivation; lysis buffer SARS-CoV-2; coronavirus; extraction buffer; COVID-19; inactivation; lysis buffer
MDPI and ACS Style

Pastorino, B.; Touret, F.; Gilles, M.; Luciani, L.; de Lamballerie, X.; Charrel, R.N. Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples. Viruses 2020, 12, 624. https://0-doi-org.brum.beds.ac.uk/10.3390/v12060624

AMA Style

Pastorino B, Touret F, Gilles M, Luciani L, de Lamballerie X, Charrel RN. Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples. Viruses. 2020; 12(6):624. https://0-doi-org.brum.beds.ac.uk/10.3390/v12060624

Chicago/Turabian Style

Pastorino, Boris; Touret, Franck; Gilles, Magali; Luciani, Lea; de Lamballerie, Xavier; Charrel, Remi N. 2020. "Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious Samples" Viruses 12, no. 6: 624. https://0-doi-org.brum.beds.ac.uk/10.3390/v12060624

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