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Article

Development and Evaluation of a Sensitive Bacteriophage-Based MRSA Diagnostic Screen

1
Laboratory Corporation of America Holdings, Burlington, NC 27215, USA
2
Laboratory Corporation of America Holdings, New Brighton, MN 55112, USA
*
Author to whom correspondence should be addressed.
Received: 19 May 2020 / Revised: 8 June 2020 / Accepted: 9 June 2020 / Published: 11 June 2020
(This article belongs to the Special Issue Phage-Based Pathogen Detection)
Engineered luciferase reporter bacteriophages provide specific, sensitive, rapid and low-cost detection of target bacteria and address growing diagnostic needs in multiple industries. Detection of methicillin-resistant Staphylococcus aureus (MRSA) nasal colonization and antibiotic susceptibility play a critical supportive role in preventing hospital-acquired infections and facilitating antibiotic stewardship. We describe the development and evaluation of a novel phage-based MRSA diagnostic screen for nasal swab specimens. The screen utilizes two luciferase reporter phages capable of recognizing genetically-diverse Staphylococcus aureus. The beta-lactam antibiotic cefoxitin is included to differentiate between resistant (MRSA) and susceptible organisms. The screen positively identified 97.7% of 390 clinical MRSA isolates at low bacterial concentrations. At higher inoculums, 93.5% of 123 clinical non-MRSA Staphylococcus aureus yielded appropriate negative results. Although cross-reactivity of the phage cocktail was observed with other staphylococcal and bacillus species, these false positives were absent under selective conditions. MRSA remained detectable in the presence of 38 distinct competing species and was accurately identified in 100% of 40 spiked nasal specimens. Thus, this six-hour screen sensitively detected MRSA both in vitro and in human nasal matrix. View Full-Text
Keywords: phage-based detection; Staphylococcus aureus; diagnostic screen; nasal swab; luciferase reporter phage; MRSA; bacteriophage phage-based detection; Staphylococcus aureus; diagnostic screen; nasal swab; luciferase reporter phage; MRSA; bacteriophage
MDPI and ACS Style

Brown, M.; Hahn, W.; Bailey, B.; Hall, A.; Rodriguez, G.; Zahn, H.; Eisenberg, M.; Erickson, S. Development and Evaluation of a Sensitive Bacteriophage-Based MRSA Diagnostic Screen. Viruses 2020, 12, 631. https://0-doi-org.brum.beds.ac.uk/10.3390/v12060631

AMA Style

Brown M, Hahn W, Bailey B, Hall A, Rodriguez G, Zahn H, Eisenberg M, Erickson S. Development and Evaluation of a Sensitive Bacteriophage-Based MRSA Diagnostic Screen. Viruses. 2020; 12(6):631. https://0-doi-org.brum.beds.ac.uk/10.3390/v12060631

Chicago/Turabian Style

Brown, Matthew; Hahn, Wendy; Bailey, Bryant; Hall, Alex; Rodriguez, Gema; Zahn, Henriett; Eisenberg, Marcia; Erickson, Stephen. 2020. "Development and Evaluation of a Sensitive Bacteriophage-Based MRSA Diagnostic Screen" Viruses 12, no. 6: 631. https://0-doi-org.brum.beds.ac.uk/10.3390/v12060631

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Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

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