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Article

Prolonged Detection of Bovine Viral Diarrhoea Virus Infection in the Semen of Bulls

Virology Laboratory, Elizabeth Macarthur Agriculture Institute, Woodbridge Road, Menangle, New South, Wales 2568, Australia
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Author to whom correspondence should be addressed.
Received: 28 May 2020 / Revised: 15 June 2020 / Accepted: 16 June 2020 / Published: 22 June 2020
(This article belongs to the Special Issue Bovine Viral Diarrhea Virus and Related Pestiviruses)
Infection of bulls with bovine viral diarrhoea virus (BVDV) can result in the development of virus persistence, confined to the reproductive tract. These bulls develop a normal immune response with high neutralizing antibody titres. However, BVDV can be excreted in the semen for a prolonged period. Although relatively rare, in this study we describe six separate cases in bulls being prepared for admission to artificial breeding centres. Semen samples were tested in a pan-Pestivirus-reactive real-time PCR assay and viral RNA was detected in semen from five of the bulls for three to eight months after infection. In one bull, virus was detected at low levels for more than five years. This bull was found to have one small testis. When slaughtered, virus was only detected in the abnormal testis. The low levels of BVDV in the semen of these bulls were only intermittently detected by virus isolation in cell culture. This virus-contaminated semen presents a biosecurity risk and confirms the need to screen all batches of semen from bulls that have been previously infected with BVDV. The use of real-time PCR is recommended as the preferred laboratory assay for this purpose. View Full-Text
Keywords: bovine viral diarrhoea virus; Pestivirus; persistent testicular infection; prolonged testicular infection; bovine; testes; semen bovine viral diarrhoea virus; Pestivirus; persistent testicular infection; prolonged testicular infection; bovine; testes; semen
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MDPI and ACS Style

Read, A.J.; Gestier, S.; Parrish, K.; Finlaison, D.S.; Gu, X.; O’Connor, T.W.; Kirkland, P.D. Prolonged Detection of Bovine Viral Diarrhoea Virus Infection in the Semen of Bulls. Viruses 2020, 12, 674. https://0-doi-org.brum.beds.ac.uk/10.3390/v12060674

AMA Style

Read AJ, Gestier S, Parrish K, Finlaison DS, Gu X, O’Connor TW, Kirkland PD. Prolonged Detection of Bovine Viral Diarrhoea Virus Infection in the Semen of Bulls. Viruses. 2020; 12(6):674. https://0-doi-org.brum.beds.ac.uk/10.3390/v12060674

Chicago/Turabian Style

Read, Andrew J., Sarah Gestier, Kate Parrish, Deborah S. Finlaison, Xingnian Gu, Tiffany W. O’Connor, and Peter D. Kirkland 2020. "Prolonged Detection of Bovine Viral Diarrhoea Virus Infection in the Semen of Bulls" Viruses 12, no. 6: 674. https://0-doi-org.brum.beds.ac.uk/10.3390/v12060674

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