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Article

The C962R ORF of African Swine Fever Strain Georgia Is Non-Essential and Not Required for Virulence in Swine

1
Plum Island Animal Disease Center, Agricultural Research Service (ARS), United States Department of Agriculture (USDA), Greenport, NY 11944, USA
2
Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06269, USA
3
Department of Pathobiology and Population Medicine, Mississippi State University, P.O. Box 6100, Starkville, MS 39762, USA
4
Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN 37830, USA
5
Department of Anatomy and Physiology, Kansas State University, Manhattan, KS 66506, USA
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 29 May 2020 / Revised: 16 June 2020 / Accepted: 18 June 2020 / Published: 23 June 2020
(This article belongs to the Special Issue Endemic and Emerging Swine Viruses)
African swine fever virus (ASFV) is the causative agent of the African swine fever (ASF) epizootic currently affecting pigs throughout Eurasia, causing significant economic losses in the swine industry. The virus genome encodes for more than 160 genes, of which only a few have been studied in detail. Here we describe the previously uncharacterized ASFV open reading frame (ORF) C962R, a gene encoding for a putative NTPase. RNA transcription studies using infected swine macrophages demonstrate that the C962R gene is translated as a late virus protein. A recombinant ASFV lacking the C962R gene (ASFV-G-ΔC962R) demonstrates in vivo that the C962R gene is non-essential, since ASFV-G-ΔC962R has similar replication kinetics in primary swine macrophage cell cultures when compared to parental highly virulent field isolate Georgia2007 (ASFV-G). Experimental infection of domestic pigs with ASFV-G-ΔC962R produced a clinical disease similar to that caused by the parental ASFV-G, confirming that deletion of the C962R gene from the ASFV genome does not impact virulence. View Full-Text
Keywords: ASFV; ASF; African swine fever virus; C962R ASFV; ASF; African swine fever virus; C962R
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MDPI and ACS Style

Ramirez-Medina, E.; Vuono, E.A.; Rai, A.; Pruitt, S.; Silva, E.; Velazquez-Salinas, L.; Zhu, J.; Borca, M.V.; Gladue, D.P. The C962R ORF of African Swine Fever Strain Georgia Is Non-Essential and Not Required for Virulence in Swine. Viruses 2020, 12, 676. https://0-doi-org.brum.beds.ac.uk/10.3390/v12060676

AMA Style

Ramirez-Medina E, Vuono EA, Rai A, Pruitt S, Silva E, Velazquez-Salinas L, Zhu J, Borca MV, Gladue DP. The C962R ORF of African Swine Fever Strain Georgia Is Non-Essential and Not Required for Virulence in Swine. Viruses. 2020; 12(6):676. https://0-doi-org.brum.beds.ac.uk/10.3390/v12060676

Chicago/Turabian Style

Ramirez-Medina, Elizabeth., Elizabeth. A. Vuono, Ayushi. Rai, Sarah. Pruitt, Ediane. Silva, Lauro. Velazquez-Salinas, James. Zhu, Manuel. V. Borca, and Douglas. P. Gladue 2020. "The C962R ORF of African Swine Fever Strain Georgia Is Non-Essential and Not Required for Virulence in Swine" Viruses 12, no. 6: 676. https://0-doi-org.brum.beds.ac.uk/10.3390/v12060676

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