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Article

Heat Inactivation of Different Types of SARS-CoV-2 Samples: What Protocols for Biosafety, Molecular Detection and Serological Diagnostics?

Unité des Virus Émergents (UVE: Aix-Marseille Univ-IRD 190-Inserm 1207-IHU Méditerranée Infection), Aix-Marseille University, 13005 Marseille, France
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 18 May 2020 / Revised: 30 June 2020 / Accepted: 6 July 2020 / Published: 7 July 2020
(This article belongs to the Special Issue Pathogenesis of Human and Animal Coronaviruses)
Standard precautions to minimize the risk of SARS-CoV-2 transmission implies that infected cell cultures and clinical specimens may undergo some sort of inactivation to reduce or abolish infectivity. We evaluated three heat inactivation protocols (56 °C-30 min, 60 °C-60 min and 92 °C-15 min) on SARS-CoV-2 using (i) infected cell culture supernatant, (ii) virus-spiked human sera (iii) and nasopharyngeal samples according to the recommendations of the European norm NF EN 14476-A2. Regardless of the protocol and the type of samples, a 4 Log10 TCID50 reduction was observed. However, samples containing viral loads > 6 Log10 TCID50 were still infectious after 56 °C-30 min and 60 °C-60 min, although infectivity was < 10 TCID50. The protocols 56 °C-30 min and 60 °C-60 min had little influence on the RNA copies detection, whereas 92 °C-15 min drastically reduced the limit of detection, which suggests that this protocol should be avoided for inactivation ahead of molecular diagnostics. Lastly, 56 °C-30 min treatment of serum specimens had a negligible influence on the results of IgG detection using a commercial ELISA test, whereas a drastic decrease in neutralizing titers was observed. View Full-Text
Keywords: SARS-CoV-2; coronavirus; heat inactivation; COVID-19; serology; ELISA; neutralization; virus neutralization test SARS-CoV-2; coronavirus; heat inactivation; COVID-19; serology; ELISA; neutralization; virus neutralization test
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MDPI and ACS Style

Pastorino, B.; Touret, F.; Gilles, M.; de Lamballerie, X.; Charrel, R.N. Heat Inactivation of Different Types of SARS-CoV-2 Samples: What Protocols for Biosafety, Molecular Detection and Serological Diagnostics? Viruses 2020, 12, 735. https://0-doi-org.brum.beds.ac.uk/10.3390/v12070735

AMA Style

Pastorino B, Touret F, Gilles M, de Lamballerie X, Charrel RN. Heat Inactivation of Different Types of SARS-CoV-2 Samples: What Protocols for Biosafety, Molecular Detection and Serological Diagnostics? Viruses. 2020; 12(7):735. https://0-doi-org.brum.beds.ac.uk/10.3390/v12070735

Chicago/Turabian Style

Pastorino, Boris; Touret, Franck; Gilles, Magali; de Lamballerie, Xavier; Charrel, Remi N. 2020. "Heat Inactivation of Different Types of SARS-CoV-2 Samples: What Protocols for Biosafety, Molecular Detection and Serological Diagnostics?" Viruses 12, no. 7: 735. https://0-doi-org.brum.beds.ac.uk/10.3390/v12070735

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