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Article

Transmission of the Bean-Associated Cytorhabdovirus by the Whitefly Bemisia tabaci MEAM1

1
Embrapa Recursos Genéticos e Biotecnologia, Brasília DF 70770-017, Brazil
2
Departamento de Fitopatologia, Instituto de Biologia, Universidade de Brasília, Brasília DF 70275-970, Brazil
3
Departamento de Biologia Celular, Instituto de Biologia, Universidade de Brasília, Brasília DF 70275-970, Brazil
4
Departamento de Fitopatologia, Escola Superior de Agricultura Luiz de Queiroz, Piracicaba SP 13418-900, Brazil
5
The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine School of Life Sciences, Arizona State University, Tempe, AZ 85287-5001, USA
6
Embrapa Arroz e Feijão, Goiânia GO 75375-000, Brazil
7
Structural Biology Research Unit, Department of Integrative Biomedical Sciences, University of Cape Town, Observatory, Cape Town 7701, South Africa
*
Authors to whom correspondence should be addressed.
Received: 4 August 2020 / Revised: 4 September 2020 / Accepted: 11 September 2020 / Published: 15 September 2020
(This article belongs to the Special Issue Plant Virus Emergence)
The knowledge of genomic data of new plant viruses is increasing exponentially; however, some aspects of their biology, such as vectors and host range, remain mostly unknown. This information is crucial for the understanding of virus–plant interactions, control strategies, and mechanisms to prevent outbreaks. Typically, rhabdoviruses infect monocot and dicot plants and are vectored in nature by hemipteran sap-sucking insects, including aphids, leafhoppers, and planthoppers. However, several strains of a potentially whitefly-transmitted virus, papaya cytorhabdovirus, were recently described: (i) bean-associated cytorhabdovirus (BaCV) in Brazil, (ii) papaya virus E (PpVE) in Ecuador, and (iii) citrus-associated rhabdovirus (CiaRV) in China. Here, we examine the potential of the Bemisia tabaci Middle East-Asia Minor 1 (MEAM1) to transmit BaCV, its morphological and cytopathological characteristics, and assess the incidence of BaCV across bean producing areas in Brazil. Our results show that BaCV is efficiently transmitted, in experimental conditions, by B. tabaci MEAM1 to bean cultivars, and with lower efficiency to cowpea and soybean. Moreover, we detected BaCV RNA in viruliferous whiteflies but we were unable to visualize viral particles or viroplasm in the whitefly tissues. BaCV could not be singly isolated for pathogenicity tests, identification of the induced symptoms, and the transmission assay. BaCV was detected in five out of the seven states in Brazil included in our study, suggesting that it is widely distributed throughout bean producing areas in the country. This is the first report of a whitefly-transmitted rhabdovirus. View Full-Text
Keywords: common bean; Phaseolus vulgaris; cytorhabdovirus; whitefly; Bemisia tabaci; vector; virus transmission; virus evolution common bean; Phaseolus vulgaris; cytorhabdovirus; whitefly; Bemisia tabaci; vector; virus transmission; virus evolution
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MDPI and ACS Style

Pinheiro-Lima, B.; Pereira-Carvalho, R.C.; Alves-Freitas, D.M.T.; Kitajima, E.W.; Vidal, A.H.; Lacorte, C.; Godinho, M.T.; Fontenele, R.S.; Faria, J.C.; Abreu, E.F.M.; Varsani, A.; Ribeiro, S.G.; Melo, F.L. Transmission of the Bean-Associated Cytorhabdovirus by the Whitefly Bemisia tabaci MEAM1. Viruses 2020, 12, 1028. https://0-doi-org.brum.beds.ac.uk/10.3390/v12091028

AMA Style

Pinheiro-Lima B, Pereira-Carvalho RC, Alves-Freitas DMT, Kitajima EW, Vidal AH, Lacorte C, Godinho MT, Fontenele RS, Faria JC, Abreu EFM, Varsani A, Ribeiro SG, Melo FL. Transmission of the Bean-Associated Cytorhabdovirus by the Whitefly Bemisia tabaci MEAM1. Viruses. 2020; 12(9):1028. https://0-doi-org.brum.beds.ac.uk/10.3390/v12091028

Chicago/Turabian Style

Pinheiro-Lima, Bruna, Rita C. Pereira-Carvalho, Dione M.T. Alves-Freitas, Elliot W. Kitajima, Andreza H. Vidal, Cristiano Lacorte, Marcio T. Godinho, Rafaela S. Fontenele, Josias C. Faria, Emanuel F.M. Abreu, Arvind Varsani, Simone G. Ribeiro, and Fernando L. Melo 2020. "Transmission of the Bean-Associated Cytorhabdovirus by the Whitefly Bemisia tabaci MEAM1" Viruses 12, no. 9: 1028. https://0-doi-org.brum.beds.ac.uk/10.3390/v12091028

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