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Article

Structure of the Capsid Size-Determining Scaffold of “Satellite” Bacteriophage P4

Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294, USA
*
Author to whom correspondence should be addressed.
Current address: Office of Research Compliance, The University of Alabama, Tuscaloosa, AL 35487, USA.
Received: 13 August 2020 / Revised: 25 August 2020 / Accepted: 26 August 2020 / Published: 27 August 2020
(This article belongs to the Special Issue In Memory of Michael Rossmann)
P4 is a mobile genetic element (MGE) that can exist as a plasmid or integrated into its Escherichia coli host genome, but becomes packaged into phage particles by a helper bacteriophage, such as P2. P4 is the original example of what we have termed “molecular piracy”, the process by which one MGE usurps the life cycle of another for its own propagation. The P2 helper provides most of the structural gene products for assembly of the P4 virion. However, when P4 is mobilized by P2, the resulting capsids are smaller than those normally formed by P2 alone. The P4-encoded protein responsible for this size change is called Sid, which forms an external scaffolding cage around the P4 procapsids. We have determined the high-resolution structure of P4 procapsids, allowing us to build an atomic model for Sid as well as the gpN capsid protein. Sixty copies of Sid form an intertwined dodecahedral cage around the T = 4 procapsid, making contact with only one out of the four symmetrically non-equivalent copies of gpN. Our structure provides a basis for understanding the sir mutants in gpN that prevent small capsid formation, as well as the nms “super-sid” mutations that counteract the effect of the sir mutations, and suggests a model for capsid size redirection by Sid. View Full-Text
Keywords: Caudovirales; mobile genetic elements; molecular piracy; bacteriophage P2; capsid assembly; size determination; Psu; Sid; sir mutants Caudovirales; mobile genetic elements; molecular piracy; bacteriophage P2; capsid assembly; size determination; Psu; Sid; sir mutants
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MDPI and ACS Style

Kizziah, J.L.; Rodenburg, C.M.; Dokland, T. Structure of the Capsid Size-Determining Scaffold of “Satellite” Bacteriophage P4. Viruses 2020, 12, 953. https://0-doi-org.brum.beds.ac.uk/10.3390/v12090953

AMA Style

Kizziah JL, Rodenburg CM, Dokland T. Structure of the Capsid Size-Determining Scaffold of “Satellite” Bacteriophage P4. Viruses. 2020; 12(9):953. https://0-doi-org.brum.beds.ac.uk/10.3390/v12090953

Chicago/Turabian Style

Kizziah, James L., Cynthia M. Rodenburg, and Terje Dokland. 2020. "Structure of the Capsid Size-Determining Scaffold of “Satellite” Bacteriophage P4" Viruses 12, no. 9: 953. https://0-doi-org.brum.beds.ac.uk/10.3390/v12090953

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