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Article

SARS-CoV-2 Nucleocapsid Protein Interacts with RIG-I and Represses RIG-Mediated IFN-β Production

1
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China
2
Guangdong Provincial Key Laboratory of Virology, Institute of Medical Microbiology, Jinan University, Guangzhou 510632, China
3
Foshan Institute of Medical Microbiology, Foshan 528315, China
*
Author to whom correspondence should be addressed.
These authors contribute equally to this work.
Academic Editor: Luis Martinez-Sobrido
Received: 20 November 2020 / Revised: 13 December 2020 / Accepted: 14 December 2020 / Published: 30 December 2020
(This article belongs to the Collection Coronaviruses)
SARS-CoV-2 is highly pathogenic in humans and poses a great threat to public health worldwide. Clinical data shows a disturbed type I interferon (IFN) response during the virus infection. In this study, we discovered that the nucleocapsid (N) protein of SARS-CoV-2 plays an important role in the inhibition of interferon beta (IFN-β) production. N protein repressed IFN-β production induced by poly(I:C) or upon Sendai virus (SeV) infection. We noted that N protein also suppressed IFN-β production, induced by several signaling molecules downstream of the retinoic acid-inducible gene I (RIG-I) pathway, which is the crucial pattern recognition receptor (PRR) responsible for identifying RNA viruses. Moreover, our data demonstrated that N protein interacted with the RIG-I protein through the DExD/H domain, which has ATPase activity and plays an important role in the binding of immunostimulatory RNAs. These results suggested that SARS-CoV-2 N protein suppresses the IFN-β response through targeting the initial step, potentially the cellular PRR–RNA-recognition step in the innate immune pathway. Therefore, we propose that the SARS-CoV-2 N protein represses IFN-β production by interfering with RIG-I. View Full-Text
Keywords: coronavirus disease 2019; COVID-19; severe acute respiratory syndrome coronavirus 2; SARS-CoV-2; nucleocapsid; interferon; IFN; retinoic acid-inducible gene I; RIG-I coronavirus disease 2019; COVID-19; severe acute respiratory syndrome coronavirus 2; SARS-CoV-2; nucleocapsid; interferon; IFN; retinoic acid-inducible gene I; RIG-I
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MDPI and ACS Style

Chen, K.; Xiao, F.; Hu, D.; Ge, W.; Tian, M.; Wang, W.; Pan, P.; Wu, K.; Wu, J. SARS-CoV-2 Nucleocapsid Protein Interacts with RIG-I and Represses RIG-Mediated IFN-β Production. Viruses 2021, 13, 47. https://0-doi-org.brum.beds.ac.uk/10.3390/v13010047

AMA Style

Chen K, Xiao F, Hu D, Ge W, Tian M, Wang W, Pan P, Wu K, Wu J. SARS-CoV-2 Nucleocapsid Protein Interacts with RIG-I and Represses RIG-Mediated IFN-β Production. Viruses. 2021; 13(1):47. https://0-doi-org.brum.beds.ac.uk/10.3390/v13010047

Chicago/Turabian Style

Chen, Keli; Xiao, Feng; Hu, Dingwen; Ge, Weiwei; Tian, Mingfu; Wang, Wenbiao; Pan, Pan; Wu, Kailang; Wu, Jianguo. 2021. "SARS-CoV-2 Nucleocapsid Protein Interacts with RIG-I and Represses RIG-Mediated IFN-β Production" Viruses 13, no. 1: 47. https://0-doi-org.brum.beds.ac.uk/10.3390/v13010047

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