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Article

Comparative Transcriptome Profiling of Human and Pig Intestinal Epithelial Cells after Porcine Deltacoronavirus Infection

1
Department of Veterinary Preventive Medicine, Food Animal Health Research Program (FAHRP), Wooster, OH 44691, USA
2
The Ohio Supercomputer Center (OSC), Columbus, OH 43212, USA
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Author to whom correspondence should be addressed.
Academic Editor: Firstname Lastname
Received: 31 December 2020 / Revised: 5 February 2021 / Accepted: 9 February 2021 / Published: 13 February 2021
(This article belongs to the Collection Coronaviruses)
Porcine deltacoronavirus (PDCoV) is an emerging infectious disease of swine with zoonotic potential. Phylogenetic analysis suggests that PDCoV originated recently from a host-switching event between birds and mammals. Little is known about how PDCoV interacts with its differing hosts. Human-derived cell lines are susceptible to PDCoV infection. Herein, we compare the gene expression profiles of an established host swine cells to potential emerging host human cells after infection with PDCoV. Cell lines derived from intestinal lineages were used to reproduce the primary sites of viral infection in the host. Porcine intestinal epithelial cells (IPEC-J2) and human intestinal epithelial cells (HIEC) were infected with PDCoV. RNA-sequencing was performed on total RNA extracted from infected cells. Human cells exhibited a more pronounced response to PDCoV infection in comparison to porcine cells with more differentially expressed genes (DEGs) in human, 7486, in comparison to pig cells, 1134. On the transcriptional level, the adoptive host human cells exhibited more DEGs in response to PDCoV infection in comparison to the primary pig host cells, where different types of cytokines can control PDCoV replication and virus production. Key immune-associated DEGs and signaling pathways are shared between human and pig cells during PDCoV infection. These included genes related to the NF-kappa-B transcription factor family, the interferon (IFN) family, the protein-kinase family, and signaling pathways such as the apoptosis signaling pathway, JAK-STAT signaling pathway, inflammation/cytokine–cytokine receptor signaling pathway. MAP4K4 was unique in up-regulated DEGs in humans in the apoptosis signaling pathway. While similarities exist between human and pig cells in many pathways, our research suggests that the adaptation of PDCoV to the porcine host required the ability to down-regulate many response pathways including the interferon pathway. Our findings provide an important foundation that contributes to an understanding of the mechanisms of PDCoV infection across different hosts. To our knowledge, this is the first report of transcriptome analysis of human cells infected by PDCoV. View Full-Text
Keywords: porcine deltacoronavirus; PDCoV; RNAseq; zoonosis; coronavirus; cross-species transmission; differentially expressed host genes; cytokines; interferons porcine deltacoronavirus; PDCoV; RNAseq; zoonosis; coronavirus; cross-species transmission; differentially expressed host genes; cytokines; interferons
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MDPI and ACS Style

Cruz-Pulido, D.; Boley, P.A.; Ouma, W.Z.; Alhamo, M.A.; Saif, L.J.; Kenney, S.P. Comparative Transcriptome Profiling of Human and Pig Intestinal Epithelial Cells after Porcine Deltacoronavirus Infection. Viruses 2021, 13, 292. https://0-doi-org.brum.beds.ac.uk/10.3390/v13020292

AMA Style

Cruz-Pulido D, Boley PA, Ouma WZ, Alhamo MA, Saif LJ, Kenney SP. Comparative Transcriptome Profiling of Human and Pig Intestinal Epithelial Cells after Porcine Deltacoronavirus Infection. Viruses. 2021; 13(2):292. https://0-doi-org.brum.beds.ac.uk/10.3390/v13020292

Chicago/Turabian Style

Cruz-Pulido, Diana; Boley, Patricia A.; Ouma, Wilberforce Z.; Alhamo, Moyasar A.; Saif, Linda J.; Kenney, Scott P. 2021. "Comparative Transcriptome Profiling of Human and Pig Intestinal Epithelial Cells after Porcine Deltacoronavirus Infection" Viruses 13, no. 2: 292. https://0-doi-org.brum.beds.ac.uk/10.3390/v13020292

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