Next Article in Journal
PhaLP: A Database for the Study of Phage Lytic Proteins and Their Evolution
Next Article in Special Issue
Resistance Evaluation of Dominant Varieties against Southern Rice Black-Streaked Dwarf Virus in Southern China
Previous Article in Journal
Histopathological Analysis of Adrenal Glands after Simian Varicella Virus Infection
Previous Article in Special Issue
Interplay of Rice Stripe Virus and Rice Black Streaked Dwarf Virus during Their Acquisition and Accumulation in Insect Vector
Article

Integrated Proteomics and Transcriptomics Analyses Reveal the Transcriptional Slippage of a Bymovirus P3N-PIPO Gene Expressed from a PVX Vector in Nicotiana benthamiana

1
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, College of Plant Protection, Hunan Agricultural University, Changsha 410128, China
2
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China
3
Cell and Molecular Sciences, James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK
*
Authors to whom correspondence should be addressed.
Academic Editors: Xifeng Wang, Anders Kvarnheden and Carl Spetz
Received: 2 June 2021 / Revised: 17 June 2021 / Accepted: 23 June 2021 / Published: 26 June 2021
(This article belongs to the Special Issue State-of-the-Art Cereal Virus Diseases in Asia and European Countries)
P3N-PIPO (P3 N-terminal fused with Pretty Interesting Potyviridae ORF), the movement protein of potyviruses, is expressed as a translational fusion with the N-terminus of P3 in potyviruses. As reported in previous studies, P3N-PIPO is expressed via transcriptional slippage at a conserved G2A6 slippery site in the genus Potyvirus. However, it is still unknown whether a similar expression mechanism of P3N-PIPO is used in the other genera of the family Potyviridae. Moreover, due to the extremely low expression level of P3N-PIPO in natural virus-infected plants, the peptides spanning the slippery site which provide direct evidence of the slippage at the protein level, have not been identified yet. In this study, a potato virus X (PVX)-based expression vector was utilized to investigate the expression mechanism of P3N-PIPO. A high expression level of the P3N-PIPO(WT) of turnip mosaic virus (TuMV, genus Potyvirus) was observed based on the PVX expression vector. For the first time, we successfully identified the peptides of P3N-PIPO spanning the slippery site by mass spectrometry. Likewise, the P3N-PIPO(WT) of wheat yellow mosaic virus (WYMV, genus Bymovirus) was also successfully expressed using the PVX expression vector. Integrated proteome and transcriptome analyses revealed that WYMV P3N-PIPO was expressed at the conserved G2A6 site through transcriptional slippage. Moreover, as revealed by mutagenesis analysis, Hexa-adenosine of the G2A6 site was important for the frameshift expression of P3N-PIPO in WYMV. According to our results, the PVX-based expression vector might be used as an excellent tool to study the expression mechanism of P3N-PIPO in Potyviridae. To the best of our knowledge, this is the first experimental evidence dissecting the expression mechanism of a bymovirus P3N-PIPO in the experimental host Nicotiana benthamiana. View Full-Text
Keywords: P3N-PIPO; transcriptional slippage; PVX; Bymovirus P3N-PIPO; transcriptional slippage; PVX; Bymovirus
Show Figures

Figure 1

MDPI and ACS Style

Yu, C.; Miao, R.; Ye, Z.; MacFarlane, S.; Lu, Y.; Li, J.; Yang, J.; Yan, F.; Dai, L.; Chen, J. Integrated Proteomics and Transcriptomics Analyses Reveal the Transcriptional Slippage of a Bymovirus P3N-PIPO Gene Expressed from a PVX Vector in Nicotiana benthamiana. Viruses 2021, 13, 1247. https://0-doi-org.brum.beds.ac.uk/10.3390/v13071247

AMA Style

Yu C, Miao R, Ye Z, MacFarlane S, Lu Y, Li J, Yang J, Yan F, Dai L, Chen J. Integrated Proteomics and Transcriptomics Analyses Reveal the Transcriptional Slippage of a Bymovirus P3N-PIPO Gene Expressed from a PVX Vector in Nicotiana benthamiana. Viruses. 2021; 13(7):1247. https://0-doi-org.brum.beds.ac.uk/10.3390/v13071247

Chicago/Turabian Style

Yu, Chulang, Runpu Miao, Zhuangxin Ye, Stuart MacFarlane, Yuwen Lu, Junmin Li, Jian Yang, Fei Yan, Liangying Dai, and Jianping Chen. 2021. "Integrated Proteomics and Transcriptomics Analyses Reveal the Transcriptional Slippage of a Bymovirus P3N-PIPO Gene Expressed from a PVX Vector in Nicotiana benthamiana" Viruses 13, no. 7: 1247. https://0-doi-org.brum.beds.ac.uk/10.3390/v13071247

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop