Next Article in Journal
Vimentin as a Cap of Invisibility: Proposed Role of Vimentin in Rabbit Hemorrhagic Disease Virus (RHDV) Infection
Previous Article in Journal
Membrane Insertion of the M13 Minor Coat Protein G3p Is Dependent on YidC and the SecAYEG Translocase
Article

Acetylation, Methylation and Allysine Modification Profile of Viral and Host Proteins during Influenza A Virus Infection

1
Department of Microbiology and Immunology, University of Otago, P.O. Box 56, Dunedin 9054, New Zealand
2
Centre for Protein Research, Research Infrastructure Centre, University of Otago, P.O. Box 56, Dunedin 9054, New Zealand
*
Author to whom correspondence should be addressed.
Academic Editor: Craig McCormick
Received: 16 June 2021 / Revised: 8 July 2021 / Accepted: 18 July 2021 / Published: 20 July 2021
(This article belongs to the Special Issue Protein and Nucleic Acid Modifications in Response to Virus Infection)
Protein modifications dynamically occur and regulate biological processes in all organisms. Towards understanding the significance of protein modifications in influenza virus infection, we performed a global mass spectrometry screen followed by bioinformatics analyses of acetylation, methylation and allysine modification in human lung epithelial cells in response to influenza A virus infection. We discovered 8 out of 10 major viral proteins and 245 out of 2280 host proteins detected to be differentially modified by three modifications in infected cells. Some of the identified proteins were modified on multiple amino acids residues and by more than one modification; the latter occurred either on different or same residues. Most of the modified residues in viral proteins were conserved across >40 subtypes of influenza A virus, and influenza B or C viruses and located on the protein surface. Importantly, many of those residues have already been determined to be critical for the influenza A virus. Similarly, many modified residues in host proteins were conserved across influenza A virus hosts like humans, birds, and pigs. Finally, host proteins undergoing the three modifications clustered in common functional networks of metabolic, cytoskeletal, and RNA processes, all of which are known to be exploited by the influenza A virus. View Full-Text
Keywords: influenza A virus; proteomic; protein modifications; methylation; acetylation; allysine influenza A virus; proteomic; protein modifications; methylation; acetylation; allysine
Show Figures

Figure 1

MDPI and ACS Style

Ahmed, F.; Kleffmann, T.; Husain, M. Acetylation, Methylation and Allysine Modification Profile of Viral and Host Proteins during Influenza A Virus Infection. Viruses 2021, 13, 1415. https://0-doi-org.brum.beds.ac.uk/10.3390/v13071415

AMA Style

Ahmed F, Kleffmann T, Husain M. Acetylation, Methylation and Allysine Modification Profile of Viral and Host Proteins during Influenza A Virus Infection. Viruses. 2021; 13(7):1415. https://0-doi-org.brum.beds.ac.uk/10.3390/v13071415

Chicago/Turabian Style

Ahmed, Farjana, Torsten Kleffmann, and Matloob Husain. 2021. "Acetylation, Methylation and Allysine Modification Profile of Viral and Host Proteins during Influenza A Virus Infection" Viruses 13, no. 7: 1415. https://0-doi-org.brum.beds.ac.uk/10.3390/v13071415

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop