Next Article in Journal
Coxsackievirus A2 Leads to Heart Injury in a Neonatal Mouse Model
Previous Article in Journal
Interaction of Poliovirus Capsid Proteins with the Cellular Autophagy Pathway
Article

Efficient Transendothelial Migration of Latently HIV-1-Infected Cells

by and *
Graduate School of Infection Control Sciences, Kitasato University, Shirokane 5-9-1, Minato-ku, Tokyo 108-8641, Japan
*
Author to whom correspondence should be addressed.
Present address: Hirota, Nagae and Associates, 4th Floor, TS Bldg., Yoyogi 1-24-10, Shibuya-ku, Tokyo 151-0053, Japan.
Academic Editor: Ester Ballana Guix
Received: 7 July 2021 / Revised: 7 August 2021 / Accepted: 9 August 2021 / Published: 11 August 2021
(This article belongs to the Section Human Virology and Viral Diseases)
A small fraction of HIV-1-infected T cells forms populations of latently infected cells when they are a naive T-cell subset or in transit to a resting memory state. Latently HIV-1-infected cells reside in lymphoid tissues and serve as viral reservoirs. However, whether they systemically recirculate in the body and re-enter the lymphoid nodes are unknown. Here, we employed two in-vitro cell coculture systems mimicking the lymphatic endothelium in lymph nodes and investigated the homing potential, specifically the transendothelial migration (TEM), of two latently HIV-1-infected cell lines (J1.1 and ACH-2). In trans-well coculture systems, J1.1 and ACH-2 showed higher TEM efficiencies than their parental uninfected and acutely infected cells. The efficiency of TEM was enhanced by the presence of stromal cells, such as HS-5 and fibroblastic reticular cells. In an in-vitro reconstituted, three-dimensional coculture system in which stromal cells are embedded in collagen matrices, J1.1 showed slightly higher TEM efficiency in the presence of HS-5. In accordance with these phenotypes, latently infected cells adhered to the endothelial cells more efficiently than uninfected cells. Together, our study showed that latently HIV-1-infected cells enhanced cell adhesion and TEM abilities, suggesting their potential for efficient homing to lymph nodes. View Full-Text
Keywords: transmigration; lymphocyte homing; reconstitution; HIV-1; latent infection; stromal cells transmigration; lymphocyte homing; reconstitution; HIV-1; latent infection; stromal cells
Show Figures

Figure 1

MDPI and ACS Style

Tanabe, R.; Morikawa, Y. Efficient Transendothelial Migration of Latently HIV-1-Infected Cells. Viruses 2021, 13, 1589. https://0-doi-org.brum.beds.ac.uk/10.3390/v13081589

AMA Style

Tanabe R, Morikawa Y. Efficient Transendothelial Migration of Latently HIV-1-Infected Cells. Viruses. 2021; 13(8):1589. https://0-doi-org.brum.beds.ac.uk/10.3390/v13081589

Chicago/Turabian Style

Tanabe, Reou, and Yuko Morikawa. 2021. "Efficient Transendothelial Migration of Latently HIV-1-Infected Cells" Viruses 13, no. 8: 1589. https://0-doi-org.brum.beds.ac.uk/10.3390/v13081589

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop